Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121003
Name   oriT_pWP8-S18-CRE-01_2 in_silico
Organism   Aeromonas caviae strain WP8-S18-CRE-01
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP022243 (25974..26111 [-], 138 nt)
oriT length   138 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 138 nt

>oriT_pWP8-S18-CRE-01_2
CGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATCCTGTATTGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13252 GenBank   WP_033996285
Name   traI_H7R78_RS22510_pWP8-S18-CRE-01_2 insolico UniProt ID   A0A0U2DHM7
Length   890 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 890 a.a.        Molecular weight: 101454.99 Da        Isoelectric Point: 9.8485

>WP_033996285.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIVKKVKNPQKAASKAVRVSRLTDYIREPERENSQEKCIHAGARGFITDEPQSQTAEMIALSQEAVRSKD
TINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINN
GFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAI
EDGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQR
QQVAQREPEPIKPDVPGWKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKG
EVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
ASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGS
FTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRV
TSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKL
ERLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
KPKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
YQRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(19-273)

Large polyvalent protein-associated domain 7 (LPD7)

(439-527)

RepB DNA-primase from phage plasmid (RepB_primase)

(549-807)


  Protein structure


Source ID Structure
AlphaFold DB A0A0U2DHM7


Host bacterium

ID   21432 GenBank   NZ_AP022243
Plasmid name   pWP8-S18-CRE-01_2 Incompatibility group   IncP6
Plasmid size   53629 bp Coordinate of oriT [Strand]   25974..26111 [-]
Host baterium   Aeromonas caviae strain WP8-S18-CRE-01

Cargo genes

Drug resistance gene   mph(A), blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -