Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120977 |
| Name | oriT_pKqs_07A044T_1  |
| Organism | Klebsiella quasipneumoniae subsp. similipneumoniae strain 07A044T |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP084788 (6515..6564 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKqs_07A044T_1
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13236 | GenBank | WP_032456792 |
| Name | traI_LGM25_RS24605_pKqs_07A044T_1 |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190686.62 Da Isoelectric Point: 6.0898
>WP_032456792.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGAFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHDARRENGETGREEITL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VDRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGAFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHDARRENGETGREEITL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VDRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 15515 | GenBank | WP_032456791 |
| Name | traD_LGM25_RS24600_pKqs_07A044T_1 |
UniProt ID | _ |
| Length | 767 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 767 a.a. Molecular weight: 85712.78 Da Isoelectric Point: 5.1149
>WP_032456791.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPISQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATESPVLRVTTVPLIKPKAAAAATAVATASSAGTPAAGGTEQE
LAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDEVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPISQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATESPVLRVTTVPLIKPKAAAAATAVATASSAGTPAAGGTEQE
LAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDEVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5957..33903
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LGM25_RS24410 (LGM25_24455) | 2002..2382 | + | 381 | WP_032437935 | hypothetical protein | - |
| LGM25_RS24415 (LGM25_24460) | 2449..2796 | + | 348 | WP_032456819 | hypothetical protein | - |
| LGM25_RS24420 (LGM25_24465) | 2891..3037 | + | 147 | WP_032437931 | hypothetical protein | - |
| LGM25_RS24425 (LGM25_24470) | 3088..3921 | + | 834 | WP_032456820 | N-6 DNA methylase | - |
| LGM25_RS24430 (LGM25_24475) | 4741..5562 | + | 822 | WP_004182076 | DUF932 domain-containing protein | - |
| LGM25_RS24435 (LGM25_24480) | 5595..5924 | + | 330 | WP_071996722 | DUF5983 family protein | - |
| LGM25_RS24440 (LGM25_24485) | 5957..6442 | - | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| LGM25_RS24445 (LGM25_24490) | 6877..7269 | + | 393 | WP_004206766 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| LGM25_RS24450 (LGM25_24495) | 7476..8171 | + | 696 | WP_074187711 | transcriptional regulator TraJ family protein | - |
| LGM25_RS24455 (LGM25_24500) | 8255..8626 | + | 372 | WP_004208838 | TraY domain-containing protein | - |
| LGM25_RS24460 (LGM25_24505) | 8680..9048 | + | 369 | WP_023307550 | type IV conjugative transfer system pilin TraA | - |
| LGM25_RS24465 (LGM25_24510) | 9062..9367 | + | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| LGM25_RS24470 (LGM25_24515) | 9387..9953 | + | 567 | WP_004194424 | type IV conjugative transfer system protein TraE | traE |
| LGM25_RS24475 (LGM25_24520) | 9940..10680 | + | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| LGM25_RS24480 (LGM25_24525) | 10680..12104 | + | 1425 | WP_032456781 | F-type conjugal transfer pilus assembly protein TraB | traB |
| LGM25_RS24485 (LGM25_24530) | 12177..12746 | + | 570 | WP_032456782 | type IV conjugative transfer system lipoprotein TraV | traV |
| LGM25_RS24490 (LGM25_24535) | 12901..13332 | + | 432 | WP_224222654 | hypothetical protein | - |
| LGM25_RS24495 (LGM25_24540) | 13356..13574 | + | 219 | WP_032456783 | hypothetical protein | - |
| LGM25_RS24500 (LGM25_24545) | 13575..13886 | + | 312 | WP_032456784 | hypothetical protein | - |
| LGM25_RS24505 (LGM25_24550) | 13953..14357 | + | 405 | WP_032456785 | hypothetical protein | - |
| LGM25_RS24510 (LGM25_24555) | 14400..14789 | + | 390 | WP_071996724 | hypothetical protein | - |
| LGM25_RS24515 (LGM25_24560) | 14797..15195 | + | 399 | WP_169735290 | hypothetical protein | - |
| LGM25_RS24520 (LGM25_24565) | 15267..17906 | + | 2640 | WP_032456787 | type IV secretion system protein TraC | virb4 |
| LGM25_RS24525 (LGM25_24570) | 17906..18295 | + | 390 | WP_004152591 | type-F conjugative transfer system protein TrbI | - |
| LGM25_RS24530 (LGM25_24575) | 18295..18921 | + | 627 | WP_004152590 | type-F conjugative transfer system protein TraW | traW |
| LGM25_RS24535 (LGM25_24580) | 18965..19924 | + | 960 | WP_015065634 | conjugal transfer pilus assembly protein TraU | traU |
| LGM25_RS24540 (LGM25_24585) | 19937..20575 | + | 639 | WP_032456788 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| LGM25_RS24545 (LGM25_24590) | 20634..22589 | + | 1956 | WP_032456789 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| LGM25_RS24550 (LGM25_24595) | 22621..22857 | + | 237 | WP_032456790 | conjugal transfer protein TrbE | - |
| LGM25_RS25180 | 22854..23039 | + | 186 | WP_032437912 | hypothetical protein | - |
| LGM25_RS24555 (LGM25_24600) | 23085..23411 | + | 327 | WP_004152685 | hypothetical protein | - |
| LGM25_RS24560 (LGM25_24605) | 23432..24184 | + | 753 | WP_015065637 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| LGM25_RS24565 (LGM25_24610) | 24195..24434 | + | 240 | WP_004152687 | type-F conjugative transfer system pilin chaperone TraQ | - |
| LGM25_RS24570 (LGM25_24615) | 24406..24963 | + | 558 | WP_032437911 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| LGM25_RS24575 (LGM25_24620) | 25008..25451 | + | 444 | WP_015065638 | F-type conjugal transfer protein TrbF | - |
| LGM25_RS24580 (LGM25_24625) | 25429..26808 | + | 1380 | WP_011977731 | conjugal transfer pilus assembly protein TraH | traH |
| LGM25_RS24585 (LGM25_24630) | 26808..29630 | + | 2823 | WP_023320108 | conjugal transfer mating-pair stabilization protein TraG | traG |
| LGM25_RS24590 (LGM25_24635) | 29654..30256 | + | 603 | WP_023280878 | hypothetical protein | - |
| LGM25_RS24595 (LGM25_24640) | 30507..31238 | + | 732 | WP_032415736 | conjugal transfer complement resistance protein TraT | - |
| LGM25_RS24600 (LGM25_24645) | 31600..33903 | + | 2304 | WP_032456791 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 21406 | GenBank | NZ_CP084788 |
| Plasmid name | pKqs_07A044T_1 | Incompatibility group | IncFIA |
| Plasmid size | 104756 bp | Coordinate of oriT [Strand] | 6515..6564 [-] |
| Host baterium | Klebsiella quasipneumoniae subsp. similipneumoniae strain 07A044T |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |