Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 120954
• Name: oriT_pSER10C-4
• Organism: Staphylococcus epidermidis strain R10C
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP091010 (4247..4367 [+], 121 nt)
• oriT length: 121 nt
• IRs (inverted repeats): 53..59, 63..69 (TTGGGGA..TCCCCAA) 22..29, 34..41 (ATTTTTTC..GAAAAAAT) 1..8, 14..21 (AGTGGCTA..TAGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 121 nt

>oriT_pSER10C-4
AGTGGCTAGCAATTAGCCACTATTTTTTCGTTAGAAAAAATCCATAAGGGGCTTGGGGACAATCCCCAACAAGCTGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_KB224_RS12465_pSER10C-4

• ID: 13226
• GenBank: WP_235063572
• Name: Relaxase_KB224_RS12465_pSER10C-4
• UniProt ID: _
• Length: 336 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 39018.95 Da
• Isoelectric Point: 7.9179

>WP_235063572.1 relaxase/mobilization nuclease domain-containing protein [Staphylococcus epidermidis]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKATRELYGKTDDNQGHVIIQSFKPDEVTPE
QCNQLGLELAEKMAPNHQVTVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRDLRDFNDEVCREHGL
SVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSQATNMDEFKDHLKQYGITIERVTPKSI
TYRHLAENKKVRGRKLGEDYNKGGIQHGFERQIQQRQQQKYESNTNFQPKRSTSNRHESTERDTDVTQSD
WNQFAQDTNELERRRQAAESARLVDEKARRDREKRVREKQASRPIINNDRDHDLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

No available structure.

Host bacterium

• ID: 21383
• GenBank: NZ_CP091010
• Plasmid name: pSER10C-4
• Incompatibility group: -
• Plasmid size: 7169 bp
• Coordinate of oriT [Strand]: 4247..4367 [+]
• Host baterium: Staphylococcus epidermidis strain R10C

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -