Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 120948
• Name: oriT_pSE50D-3
• Organism: Staphylococcus epidermidis strain 50D
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP090988 (2701..2821 [+], 121 nt)
• oriT length: 121 nt
• IRs (inverted repeats): 53..60, 62..69 (TTGGGGAT..ATCCCCAA) 22..29, 34..41 (ATTTTTTC..GAAAAAAT) 1..8, 14..21 (AGTGGCTA..TAGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 121 nt

>oriT_pSE50D-3
AGTGGCTAGCAATTAGCCACTATTTTTTCGTTAGAAAAAATCCATAAGGGGTTTGGGGATTATCCCCAACAAGCAGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_KB226_RS12000_pSE50D-3

• ID: 13220
• GenBank: WP_235075629
• Name: Relaxase_KB226_RS12000_pSE50D-3
• UniProt ID: _
• Length: 336 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 38943.03 Da
• Isoelectric Point: 8.8977

>WP_235075629.1 relaxase/mobilization nuclease domain-containing protein [Staphylococcus epidermidis]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKATRELYGKTDGNQGHVIIQSFKPDEVTPE
QCNQLGLELAEKMAPNHQVTVYTHNDTDHVHNHIVINAIDLETGKKFNNNKQTLRDLRDFNDEVCREHGL
SVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSQATNMDEFKDHLKQYGITIERVTPKSI
TYRHLAENKKVRGRKLGEDYNKGGIQHGFERQIQRRQQQKYESNTNFQPKRSTSNRHESTERDTDVTQSD
WNQFAQDTNELERRRQAAESARLVDEKARRDREKRVREKPTSRPIINIDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

No available structure.

Host bacterium

• ID: 21377
• GenBank: NZ_CP090988
• Plasmid name: pSE50D-3
• Incompatibility group: -
• Plasmid size: 5816 bp
• Coordinate of oriT [Strand]: 2701..2821 [+]
• Host baterium: Staphylococcus epidermidis strain 50D

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -