Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120941
Name   oriT_pEclC18 in_silico
Organism   Enterobacter cloacae strain C18
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MH713707 (12255..12344 [-], 90 nt)
oriT length   90 nt
IRs (inverted repeats)      34..41, 44..51  (AGTGCGAT..ATCGCACT)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 90 nt

>oriT_pEclC18
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13215 GenBank   WP_024561907
Name   mobF_L1977_RS00110_pEclC18 insolico UniProt ID   H1ZMV6
Length   1072 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1072 a.a.        Molecular weight: 118896.59 Da        Isoelectric Point: 6.4636

>WP_024561907.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(16-288)

AAA domain (AAA_30)

(480-656)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(855-899)


  Protein structure


Source ID Structure
AlphaFold DB H1ZMV6


T4CP

ID   15498 GenBank   WP_063131939
Name   t4cp2_L1977_RS00105_pEclC18 insolico UniProt ID   _
Length   510 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 510 a.a.        Molecular weight: 57872.95 Da        Isoelectric Point: 9.5852

>WP_063131939.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERGHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSMKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-492)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 53358..63646

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1977_RS00265 (pEclC18_058) 48645..48992 - 348 WP_000679427 quaternary ammonium compound efflux SMR transporter QacE delta 1 -
L1977_RS00270 (pEclC18_059) 49196..49669 - 474 WP_032490158 trimethoprim-resistant dihydrofolate reductase DfrA16 -
L1977_RS00275 (pEclC18_060) 49825..50838 + 1014 WP_000845048 class 1 integron integrase IntI1 -
L1977_RS00280 (pEclC18_061) 51122..51697 + 576 WP_063131942 recombinase family protein -
L1977_RS00285 51850..52203 - 354 WP_162478394 hypothetical protein -
L1977_RS00290 (pEclC18_064) 52834..53346 - 513 WP_029697418 phospholipase D family protein -
L1977_RS00295 (pEclC18_065) 53358..54359 - 1002 WP_001076634 ATPase, T2SS/T4P/T4SS family virB11
L1977_RS00300 (pEclC18_066) 54410..55543 - 1134 WP_000101920 type IV secretion system protein VirB10 virB10
L1977_RS00305 (pEclC18_067) 55543..56430 - 888 WP_000758230 TrbG/VirB9 family P-type conjugative transfer protein virB9
L1977_RS00310 (pEclC18_068) 56441..57136 - 696 WP_001208352 type IV secretion system protein virB8
L1977_RS00395 57147..57278 - 132 WP_032493177 hypothetical protein -
L1977_RS00315 (pEclC18_069) 57355..58392 - 1038 WP_014014948 type IV secretion system protein virB6
L1977_RS00320 (pEclC18_070) 58409..58651 - 243 WP_000858958 EexN family lipoprotein -
L1977_RS00325 (pEclC18_071) 58661..59374 - 714 WP_000644929 type IV secretion system protein virB5
L1977_RS00330 (pEclC18_072) 59390..61963 - 2574 WP_032493176 VirB4 family type IV secretion/conjugal transfer ATPase virb4
L1977_RS00335 (pEclC18_073) 61964..62281 - 318 WP_000462629 type IV secretion system protein VirB3 virB3
L1977_RS00340 (pEclC18_074) 62321..62662 - 342 WP_001121032 TrbC/VirB2 family protein virB2
L1977_RS00345 (pEclC18_075) 62864..63646 - 783 WP_024562119 lytic transglycosylase domain-containing protein virB1
L1977_RS00350 (pEclC18_077) 63710..64063 + 354 WP_225988093 H-NS family nucleoid-associated regulatory protein -
L1977_RS00355 (pEclC18_078) 64067..64417 + 351 WP_000528713 hypothetical protein -
L1977_RS00360 64396..64752 + 357 WP_014014954 hypothetical protein -
L1977_RS00365 (pEclC18_080) 64756..65064 + 309 WP_000733764 hypothetical protein -
L1977_RS00370 (pEclC18_081) 65349..65534 + 186 WP_024562121 ribbon-helix-helix domain-containing protein -
L1977_RS00375 (pEclC18_082) 65531..66139 - 609 WP_000275218 recombinase family protein -
L1977_RS00380 (pEclC18_083) 66305..66637 - 333 WP_000140165 hypothetical protein -

Host bacterium

ID   21370 GenBank   NZ_MH713707
Plasmid name   pEclC18 Incompatibility group   IncN2
Plasmid size   66776 bp Coordinate of oriT [Strand]   12255..12344 [-]
Host baterium   Enterobacter cloacae strain C18

Cargo genes

Drug resistance gene   qnrB67, blaTEM-1A, blaOXA-9, ant(3'')-Ia, aac(6')-Ib, blaPER-2, qacE, dfrA16
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -