Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120807
Name   oriT_AO-1|unnamed in_silico
Organism   Pseudomonas sp. AO-1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP079948 (41372..41798 [+], 427 nt)
oriT length   427 nt
IRs (inverted repeats)      164..170, 174..180  (GCCGCGC..GCGCGGC)
 123..128, 142..147  (GCAATT..AATTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 427 nt

>oriT_AO-1|unnamed
GGTGGTCCTCTCTGAGCCGAAATTGACGCATTTTCACAGGTACAATATCGGTCCGAACCGCGTATAGCAAATGTAATACAAAAAAAGCTTGATTTTGCTGGTCTAGATGGTACTTTTGTATAGCAATTGTATAGCGTAATAAATTGCATGGATTTCGCTGTAGGCCGCGCTCTGCGCGGCTTGCGCCTCCCGCTAGGGGGGTGCGTGATGTGTATTGAGATTTTGACCCTGTTAAGGGGAAAAATCGAAGCTCTTGTTAAGGGCGTATCCACTGTTTAGGCCGAAGGCCGTAGGGTGGTATGCAGAAAGGGTGCAGGAAGGGTGCAGGAAGGGTGCTACAAGAGTGCAAGAGCAGTGCAACTTGACTACAGGTAGGGGGCAGGTAAGATGCACATAGAGTGCAACAGGGGTGCAGGTAGGGTGCAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13121 GenBank   WP_222019483
Name   mobF_KVQ82_RS30820_AO-1|unnamed insolico UniProt ID   _
Length   981 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 981 a.a.        Molecular weight: 109279.15 Da        Isoelectric Point: 9.9333

>WP_222019483.1 MobF family relaxase [Pseudomonas sp. AO-1]
MFNVTSIKGSNQYAAAGYYTAADDYYAKESPGEWQGIGAELLGLEGPIDQQELAKLFDGKLPNGEMMPKP
VNKETGEIVTRRMGLDLTFSAPKSVSMQALIAGDKDVVAAHDRAVTKAMEHVEKLAQTRRKEHGKSMLER
TGNLIIGKFRHELSRAKDPQLHTHAVVMNATRRADGKWRAIHNDDIFKIQPQIDAMYKGELAKELRELGY
EIRVLDKDGNFELAHISRDQIEAFSSRAKVIEDALAKDGKTRSNATALEKQIIAMATRPRKDERDRHLVK
EYWVTKARELGIEFGGRSQLDNREYGRRSESIHAEHNLPEGISAGQAVVQYAINHLTEREQVVGESDLRT
AALRRAVGLASPTEVDDEIKRLVKQGSLIESPPAYRMATAPDGAALSPAGWRALLKEQKGWSEKEAQQYV
KKAIDRGSLVEVEKRYTTQRALKREKAILAIERTGRGQVTPLLTKEQVAKALEGSTLSAGQYQAVEVIVS
TSNRFVGIQGDAGTGKTYSVDRAVKLIESVNNAMTERSTTTDATFRVVALAPYGNQVTALKNEGLDAHTL
ASFFHTKDKKLDERTIVVLDEAGVVGARQMEQLMRIIEQSGARLVQLGDTKQTEAIEAGKPFAQLQQNGM
ETARIKEIQRQKNQELKIAVQHAAEGNPGKSLAHVNHVEEFRDPALRHEAIVRDYISLTPEERKEVLIVA
GTNKDRKQINTMTREALGFVGKGLELPTLNRVDTTQAERRYAPSYKKGMIVQPEKDYIRAGLTRGELYTV
DQALPGNVLVVKDKNGNRVEFNPRKLTKLSVYNLEKPEFSVGDLVRITRNDQKLDLTNGDRMRVVGNVNG
VIELASVKERDGKPERVVTLPTNRPLHLEHAYSATVHSAQGLTNDRVMISINTKSRTTSQNLWYVAISRA
RYEARIYTDKIAHLPAAIANRYDKTTALSLQQERERQRKEPIKPRTVLDGKAMEKKQRTALDGASAGKSG
V

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(476-666)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(872-918)

TrwC relaxase (TrwC)

(15-289)


  Protein structure



No available structure.




T4CP

ID   15400 GenBank   WP_017124988
Name   t4cp2_KVQ82_RS30825_AO-1|unnamed insolico UniProt ID   _
Length   518 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 518 a.a.        Molecular weight: 58205.54 Da        Isoelectric Point: 8.9125

>WP_017124988.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Pseudomonas]
MHKREVDSATMTAGFGLPIAGWGAGVYLAQMPLTPFPAAFKETLHNGWHNPIVLGATIATSVAAASLVYY
LQEYCDDGFRGEQYQEFLRGSQIKNWHTVKAKVNRRNAQTNRERKKKGLAKSDPIMIGKLPMPLHLEDRN
TMICASIGAGKSVTMEGMIASALKRGDRMAVVDPNGTFYSKFSFKGDYILNPFDVRSAGWTIFNEIRGIH
DFNRMAKSIIPPQVDPSDEQWCAYARDVLSDTMRKLKETNNPNQDTLVNLLVREDGDTIRAFLANTDSEG
YFRDNAEKAIASIQFMMNKYIRPLRYMSKGEFSIYQWVNDPNAGNLFITWREDMRDTMKPLVATWIDTIC
ATILSSEPMTGKRLWLFLDELQSLGKLESFVPAATKGRKHGLRMVGSLQDWSQLNASYGRDDAETLLSCF
RNYVILAAANAKNAGMASEILGSHDVKRWRKSHTAGKLTRTEEVTRDEPVVQASEISNLPDLVAYVKFGE
DFPITKVKTPYIDYKERAQAIMITGQAA

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(125-499)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 62528..72821

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KVQ82_RS30970 (KVQ82_30960) 58227..58856 - 630 WP_172693492 recombinase family protein -
KVQ82_RS30975 (KVQ82_30965) 59122..59538 - 417 WP_172693491 hypothetical protein -
KVQ82_RS30980 (KVQ82_30970) 59535..59873 - 339 WP_172693490 TrfB-related DNA-binding protein -
KVQ82_RS30985 (KVQ82_30975) 59882..60805 - 924 WP_172693489 ParB/RepB/Spo0J family partition protein -
KVQ82_RS30990 (KVQ82_30980) 60816..61589 - 774 WP_254170386 ParA family protein -
KVQ82_RS30995 (KVQ82_30985) 62528..62989 + 462 WP_172693488 TrbC/VirB2 family protein virB2
KVQ82_RS31000 (KVQ82_30990) 62993..63286 + 294 WP_222019490 VirB3 family type IV secretion system protein virB3
KVQ82_RS31005 (KVQ82_30995) 63273..65957 + 2685 WP_260688138 type VI secretion protein virb4
KVQ82_RS31010 (KVQ82_31000) 65957..66640 + 684 WP_042934861 type IV secretion system protein virB5
KVQ82_RS31015 (KVQ82_31005) 66668..66880 + 213 WP_017124976 hypothetical protein -
KVQ82_RS31020 (KVQ82_31010) 66873..67277 + 405 WP_222019491 hypothetical protein -
KVQ82_RS31025 (KVQ82_31015) 67265..68128 + 864 WP_017124978 type IV secretion system protein virB6
KVQ82_RS31030 (KVQ82_31020) 68128..68793 + 666 WP_017124979 type IV secretion system protein virB8
KVQ82_RS31035 (KVQ82_31025) 68820..69611 + 792 WP_172693485 TrbG/VirB9 family P-type conjugative transfer protein virB9
KVQ82_RS31040 (KVQ82_31030) 69614..70897 + 1284 WP_017124981 TrbI/VirB10 family protein virB10
KVQ82_RS31045 (KVQ82_31035) 70907..71935 + 1029 WP_017124982 P-type DNA transfer ATPase VirB11 virB11
KVQ82_RS31050 (KVQ82_31040) 71919..72821 + 903 WP_222019492 lytic transglycosylase domain-containing protein virB1
KVQ82_RS31055 (KVQ82_31045) 72851..73180 + 330 WP_017124984 TrbM/KikA/MpfK family conjugal transfer protein -
KVQ82_RS31060 (KVQ82_31050) 73170..73775 + 606 WP_017124985 phospholipase D family protein -
KVQ82_RS31065 (KVQ82_31055) 73790..74272 + 483 WP_172693483 hypothetical protein -
KVQ82_RS31070 (KVQ82_31060) 74318..74497 + 180 WP_222019493 hypothetical protein -
KVQ82_RS31075 (KVQ82_31065) 74598..75099 - 502 Protein_94 site-specific integrase -

Host bacterium

ID   21236 GenBank   NZ_CP079948
Plasmid name   AO-1|unnamed Incompatibility group   -
Plasmid size   75099 bp Coordinate of oriT [Strand]   41372..41798 [+]
Host baterium   Pseudomonas sp. AO-1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   merR1, merT, merP, merC, merA, merE, merD, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -