Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 120800
• Name: oriT_pCAV1151-296
• Organism: Phytobacter ursingii strain CAV1151
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP011601 (3384..3638 [-], 255 nt)
• oriT length: 255 nt
• IRs (inverted repeats): 153..158, 160..165 (AAAAGT..ACTTTT) 122..130, 135..143 (TTAAGGCTT..AAGCCTTAA) 49..55, 60..66 (GAATTTT..AAAATTC)
• Location of nic site: 78..79
• Conserved sequence flanking the nic site: TTTGGTTAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 255 nt

>oriT_pCAV1151-296
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobH_AB182_RS01920_pCAV1151-296

• ID: 13119
• GenBank: WP_012477394
• Name: mobH_AB182_RS01920_pCAV1151-296
• UniProt ID: A0A2Z2E7B6
• Length: 1050 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1050 a.a.
• Molecular weight: 118067.47 Da
• Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (108-304)

Protein structure

• AlphaFold DB: A0A2Z2E7B6

T4CP

traD_AB182_RS01925_pCAV1151-296

• ID: 15398
• GenBank: WP_001284073
• Name: traD_AB182_RS01925_pCAV1151-296
• UniProt ID: _
• Length: 694 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 694 a.a.
• Molecular weight: 78123.91 Da
• Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (406-609)

• AAA-like domain (AAA_10) (199-249)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 111866..121106

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB182_RS32500	109156..109317	-	162	WP_001371949	hypothetical protein	-
AB182_RS02450 (AB182_02450)	111866..114547	-	2682	WP_000387412	TraC family protein	virb4
AB182_RS02455 (AB182_02455)	114556..115506	-	951	WP_001022588	IncHI-type conjugal transfer lipoprotein TrhV	traV
AB182_RS02460 (AB182_02460)	115516..116268	-	753	WP_182911723	protein-disulfide isomerase HtdT	-
AB182_RS02465 (AB182_02465)	116387..116869	-	483	WP_000377632	hypothetical protein	-
AB182_RS02470 (AB182_02470)	116877..118232	-	1356	WP_000351841	IncHI-type conjugal transfer protein TrhB	traB
AB182_RS02475 (AB182_02475)	118222..118683	-	462	WP_000521242	plasmid transfer protein HtdO	-
AB182_RS02480 (AB182_02480)	118685..119956	-	1272	WP_000592092	type-F conjugative transfer system secretin TraK	traK
AB182_RS02485 (AB182_02485)	119956..120744	-	789	WP_000783153	TraE/TraK family type IV conjugative transfer system protein	traE
AB182_RS02490 (AB182_02490)	120756..121106	-	351	WP_001447770	type IV conjugative transfer system protein TraL	traL
AB182_RS02495 (AB182_02495)	121124..121477	-	354	WP_000423602	hypothetical protein	-
AB182_RS02505 (AB182_02505)	122626..123501	-	876	WP_000594611	RepB family plasmid replication initiator protein	-
AB182_RS02510 (AB182_02510)	123978..125030	+	1053	WP_001065778	hypothetical protein	-
AB182_RS02515 (AB182_02515)	125050..125250	+	201	WP_000594930	hypothetical protein	-
AB182_RS02520 (AB182_02520)	125247..125963	+	717	WP_000838219	zinc finger-like domain-containing protein	-

Host bacterium

• ID: 21229
• GenBank: NZ_CP011601
• Plasmid name: pCAV1151-296
• Incompatibility group: IncHI2A
• Plasmid size: 295619 bp
• Coordinate of oriT [Strand]: 3384..3638 [-]
• Host baterium: Phytobacter ursingii strain CAV1151

Cargo genes

• Drug resistance gene: catA2, aac(6')-IIc, ere(A), qacE, sul1, sul2, blaTEM-1B, aadA2, dfrA19, aph(3'')-Ib, aph(6)-Id, mcr-9, aph(3')-Ia
• Virulence gene: -
• Metal resistance gene: terE, terD, terC, terB, terA, terZ, terW, pbrA, merE, merD, merA, merC, merP, merT, merR, pcoS, pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -