Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 120775
• Name: oriT_pMCR-Z96-1
• Organism: Leclercia adecarboxylata strain Z96-1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP040891 (8580..8621 [+], 42 nt)
• oriT length: 42 nt
• IRs (inverted repeats): 7..13, 25..31 (CGTTTCT..AGAAACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 42 nt

>oriT_pMCR-Z96-1
GCGCACCGTTTCTGAACGAAGTGAAGAAACGTCTAAGTGCGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobA_FHN83_RS26280_pMCR-Z96-1

• ID: 13103
• GenBank: WP_040113349
• Name: mobA_FHN83_RS26280_pMCR-Z96-1
• UniProt ID: A0A734ICP3
• Length: 365 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 365 a.a.
• Molecular weight: 42217.70 Da
• Isoelectric Point: 6.3344

>WP_040113349.1 MULTISPECIES: mobilization protein MobA [Enterobacterales]
MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNMPAWAAHKPTEFWKAADTFERANGCT
YREIEIALPRELKPEQRLELVRDFVRQEIGDRHAYQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDP
QQYFKRANSKAPERGGAKKARFGETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIERE
PERHLGAGQVQRFDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQ
EDTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQRQQEKEQQRLA
EQTRQKPDKGWSFSR

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (42-218)

Protein structure

• AlphaFold DB: A0A734ICP3

Host bacterium

• ID: 21204
• GenBank: NZ_CP040891
• Plasmid name: pMCR-Z96-1
• Incompatibility group: ColE10
• Plasmid size: 9782 bp
• Coordinate of oriT [Strand]: 8580..8621 [+]
• Host baterium: Leclercia adecarboxylata strain Z96-1

Cargo genes

• Drug resistance gene: mcr-4.3
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -