Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120768
Name   oriT_1.2.7|unnamed in_silico
Organism   Leclercia pneumoniae strain 1.2.7
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP134504 (134394..134648 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..128, 137..143  (TTAAGGC..GCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_1.2.7|unnamed
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCATATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13096 GenBank   WP_137516387
Name   mobH_RKT74_RS22410_1.2.7|unnamed insolico UniProt ID   _
Length   1048 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1048 a.a.        Molecular weight: 117841.19 Da        Isoelectric Point: 4.5894

>WP_137516387.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPTGI
QTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGGLL
SHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWTPS
SQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVTKERVYSKMITALNVYTDG
NDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGEVY
LNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKIVTEYRRPVPGRDAITKITDTVE
TAVLKVNDLGRSSASIDVDIHSEKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESEDE
SAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGSLD
GMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLELP
PPRVNAVEEHSGDVEYHGTDSASATGQLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFTQLSISRPEVGSC
ATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAAAT
SVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPASPV
SGYNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTLYL
TQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-302)


  Protein structure



No available structure.




T4CP

ID   15372 GenBank   WP_001284073
Name   traD_RKT74_RS22415_1.2.7|unnamed insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2429..11636

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RKT74_RS21735 (RKT74_21735) 1..876 + 876 WP_263616068 RepB family plasmid replication initiator protein -
RKT74_RS21740 (RKT74_21740) 2025..2378 + 354 WP_000423602 hypothetical protein -
RKT74_RS21745 (RKT74_21745) 2429..2746 + 318 WP_000043359 type IV conjugative transfer system protein TraL traL
RKT74_RS21750 (RKT74_21750) 2758..3546 + 789 WP_185846500 TraE/TraK family type IV conjugative transfer system protein traE
RKT74_RS21755 (RKT74_21755) 3546..4817 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
RKT74_RS21760 (RKT74_21760) 4819..5280 + 462 WP_000521241 plasmid transfer protein HtdO -
RKT74_RS21765 (RKT74_21765) 5270..6625 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
RKT74_RS21770 (RKT74_21770) 6633..7115 + 483 WP_040038370 hypothetical protein -
RKT74_RS21775 (RKT74_21775) 7234..7986 + 753 WP_182911723 protein-disulfide isomerase HtdT -
RKT74_RS21780 (RKT74_21780) 7996..8946 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
RKT74_RS21785 (RKT74_21785) 8955..11636 + 2682 WP_000387412 TraC family protein virb4
RKT74_RS21790 (RKT74_21790) 13937..14098 + 162 WP_001371949 hypothetical protein -
RKT74_RS21795 (RKT74_21795) 15227..16480 + 1254 WP_001050364 ParA family protein -

Host bacterium

ID   21197 GenBank   NZ_CP134504
Plasmid name   1.2.7|unnamed Incompatibility group   IncHI2A
Plasmid size   207184 bp Coordinate of oriT [Strand]   134394..134648 [-]
Host baterium   Leclercia pneumoniae strain 1.2.7

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -