Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120764
Name   oriT_pKPC-79f0 in_silico
Organism   Leclercia sp. LSNIH1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP026169 (22104..22204 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pKPC-79f0
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13092 GenBank   WP_012561166
Name   mobF_C2U54_RS24160_pKPC-79f0 insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   7307 GenBank   WP_001749975
Name   WP_001749975_pKPC-79f0 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   15366 GenBank   WP_000342688
Name   t4cp2_C2U54_RS24165_pKPC-79f0 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 48096..58421

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U54_RS24330 (C2U54_24310) 43941..45374 - 1434 WP_001288432 DNA cytosine methyltransferase -
C2U54_RS24335 (C2U54_24315) 45473..45745 + 273 Protein_47 IS1 family transposase -
C2U54_RS24340 (C2U54_24320) 45756..45962 - 207 WP_001749967 hypothetical protein -
C2U54_RS24345 (C2U54_24325) 45967..46614 - 648 WP_012561184 restriction endonuclease -
C2U54_RS24350 (C2U54_24330) 46664..46975 - 312 WP_001452736 hypothetical protein -
C2U54_RS24355 (C2U54_24335) 47011..47325 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
C2U54_RS24360 (C2U54_24340) 47322..47666 - 345 WP_001749964 hypothetical protein -
C2U54_RS24365 (C2U54_24345) 47682..47987 - 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
C2U54_RS24370 (C2U54_24350) 48096..48830 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
C2U54_RS24375 (C2U54_24355) 48839..49120 + 282 WP_000440698 transcriptional repressor KorA -
C2U54_RS24380 (C2U54_24360) 49130..49423 + 294 WP_001749962 hypothetical protein virB2
C2U54_RS24385 (C2U54_24365) 49473..49790 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
C2U54_RS24390 (C2U54_24370) 49790..52390 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
C2U54_RS24395 (C2U54_24375) 52408..53121 + 714 WP_001749960 type IV secretion system protein virB5
C2U54_RS24400 (C2U54_24380) 53129..53356 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
C2U54_RS24405 (C2U54_24385) 53372..54412 + 1041 WP_001749958 type IV secretion system protein virB6
C2U54_RS28015 (C2U54_24390) 54513..54641 + 129 WP_071882546 conjugal transfer protein TraN -
C2U54_RS24415 (C2U54_24395) 54631..55329 + 699 WP_000646594 virB8 family protein virB8
C2U54_RS24420 (C2U54_24400) 55340..56224 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
C2U54_RS24425 (C2U54_24405) 56224..57384 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
C2U54_RS24430 (C2U54_24410) 57426..58421 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11

Host bacterium

ID   21193 GenBank   NZ_CP026169
Plasmid name   pKPC-79f0 Incompatibility group   IncN
Plasmid size   58627 bp Coordinate of oriT [Strand]   22104..22204 [+]
Host baterium   Leclercia sp. LSNIH1

Cargo genes

Drug resistance gene   blaKPC-2, dfrA14
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -