Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120733
Name   oriT_pRHBSTW-00464_2 in_silico
Organism   Klebsiella sp. RHBSTW-00464
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP056484 (181120..181403 [-], 284 nt)
oriT length   284 nt
IRs (inverted repeats)      183..188, 190..195  (AAAAGT..ACTTTT)
 45..50, 55..60  (TTTTAA..TTAAAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 284 nt

>oriT_pRHBSTW-00464_2
GTTATTGCCGCTTAATGCCGATAACGATTCGGGCTTTGCTATTTTTTTAAGCGATTAAAATTTCGTTAGCAAGCGCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTCAAAAAGTGTTACAAGTAAGGCCAATGGATGAATGGTTAGTTTTAAGACTCAAATAGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCATTCAAAAACCAAACTTTAATTTCATGTAGAAGATTAGTACAATTGTATTGACGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13070 GenBank   WP_223203175
Name   mobH_HV204_RS30725_pRHBSTW-00464_2 insolico UniProt ID   _
Length   988 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 988 a.a.        Molecular weight: 110156.87 Da        Isoelectric Point: 4.5476

>WP_223203175.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MAELMMKDVSSLSPFAQIIGDQKYTVPDHPNLEVLKFIEYPTRPAGIQTFNEQSILSLYRDKLHSISMML
AISDGDIREDAFTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLLSHSLEVAMISLKNANHSELRPIG
YQDEEVIRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLNETLTWAPSSQSLLDWARENNVVEYEIHWRKR
IHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDGNDFLSKCVRTSDYYSTGTDLNVL
RDPIMGLRSNDAAARAIGTIKHNFTSININNYKSKPMHIIIVNGEVYLNENAFLDFVLSDFAAHKFNFPQ
GDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENEIASIFRNGIGKLEFYNLLKLRWIGLIFDSYK
IPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVSDAIEDTPQYGLKLVNGPDADSVN
GKSSENTEAITESVEAAGTDKATDIIDPQVTTPTDTAGTGGLDEKERVEEHEEQSQMRLVEQLHEMLLSA
PLPHNAVINIDSVPYLNLDAAIALIPGIDEAAFCDGPFFQLTYRDGSLDGMWIVRDVNNLRLIQLGDNCA
GIQVSTSEPRTTSTLKSLFDTSMYQPLDIPESPSLNEAVSPPQTPLDLPPARLNTPQAVEANSVAEQINA
HSEPETVTASEYEQYGHLLEETLDSDSEAYSDFIVADNSVVEPPQPEAKNSDVAEFHDETDQVVAPGKLN
DPEDTREPPAPNTTGKEVILTSPVSSQDPIKSEPAGEKANHLSPALARLFAVNTQAEKKHENPPVTTPVK
EAFNRSNSATVQAPIAVDQPGAEEKETLEDFTLLSGGEVTELEYVEIATMLHHILTKLSGSFKRKRKNRF
MVLTQNTFFLTQSCVEKYGTQLNAPELFNQLPQYQVTSGAIVNTKCFAFNMPTLVAASDRAKVDIELIIN
TLKDVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(83-278)


  Protein structure



No available structure.




T4CP

ID   15339 GenBank   WP_181486533
Name   traD_HV204_RS30730_pRHBSTW-00464_2 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77876.53 Da        Isoelectric Point: 8.4994

>WP_181486533.1 MULTISPECIES: conjugative transfer system coupling protein TraD [unclassified Klebsiella]
MAKSKRTNLHAQENFYRPILEYRSASVLLICAIIMLFMGFRSDGLNIAPIILYTAAFMLLVCLYRCKTAY
PYLMAHWRVFQRQIMFISLKSLRSINKSNFFSNERKYRQLVQEYKKSNRQVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFALNPIARHFEAMARSMGGSNAIFAVDKRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDADWRQSLMDEAAELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMAILNLTVKVLECCYARL
YGPDVWMEKVKYSSNDTLQVRFKRLVEWFSAHFLNYEGAEPIDWIDTVGRLVDYSLSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNSLTSREIVTSDGIFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNSDDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSENSMRPNTTLLEMFLKPAGKVTLRQNVGLDYLNKHLRKLH

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(505-627)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2799..14366

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV204_RS29730 (HV204_29730) 1..876 + 876 WP_015063098 RepB family plasmid replication initiator protein -
HV204_RS29735 (HV204_29735) 1919..2104 + 186 WP_032610388 hypothetical protein -
HV204_RS29740 (HV204_29740) 2394..2747 + 354 WP_015063100 hypothetical protein -
HV204_RS29745 (HV204_29745) 2799..3116 + 318 WP_022652151 type IV conjugative transfer system protein TraL traL
HV204_RS29750 (HV204_29750) 3128..3913 + 786 WP_022652152 TraE/TraK family type IV conjugative transfer system protein traE
HV204_RS29755 (HV204_29755) 3916..5154 + 1239 WP_015063103 type-F conjugative transfer system secretin TraK traK
HV204_RS29760 (HV204_29760) 5156..5596 + 441 WP_015063104 plasmid transfer protein HtdO -
HV204_RS29765 (HV204_29765) 5586..6944 + 1359 WP_022652153 IncHI-type conjugal transfer protein TrhB traB
HV204_RS29770 (HV204_29770) 6953..7123 + 171 WP_228288644 hypothetical protein -
HV204_RS29775 (HV204_29775) 7169..8149 + 981 WP_000019403 IS5-like element IS5 family transposase -
HV204_RS29780 (HV204_29780) 8320..8658 + 339 WP_228288645 transcriptional regulator -
HV204_RS29785 (HV204_29785) 8764..9516 + 753 WP_185757620 protein-disulfide isomerase HtdT -
HV204_RS29790 (HV204_29790) 9526..10482 + 957 WP_181486535 TraV family lipoprotein traV
HV204_RS29795 (HV204_29795) 10523..11503 + 981 WP_000019450 IS5-like element ISKpn26 family transposase -
HV204_RS33610 11485..11676 + 192 WP_327021807 hypothetical protein -
HV204_RS29800 (HV204_29800) 11685..14366 + 2682 WP_015063109 IncHI-type conjugal transfer ATPase TrhC virb4
HV204_RS29805 (HV204_29805) 15226..15630 + 405 WP_003031976 transposase -
HV204_RS29810 (HV204_29810) 15627..15974 + 348 WP_000612626 IS66 family insertion sequence element accessory protein TnpB -
HV204_RS29815 (HV204_29815) 16023..17561 + 1539 WP_004201219 IS66-like element ISKpn24 family transposase -

Host bacterium

ID   21162 GenBank   NZ_CP056484
Plasmid name   pRHBSTW-00464_2 Incompatibility group   IncHI1A
Plasmid size   262877 bp Coordinate of oriT [Strand]   181120..181403 [-]
Host baterium   Klebsiella sp. RHBSTW-00464

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   arsC, arsB, arsR, arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -