Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 120624
• Name: oriT_pF217-MCR_313k
• Organism: Citrobacter braakii strain F217
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP136723 (122190..122444 [-], 255 nt)
• oriT length: 255 nt
• IRs (inverted repeats): 153..158, 160..165 (AAAAGT..ACTTTT) 122..130, 135..143 (TTAAGGCTT..AAGCCTTAA) 49..55, 60..66 (GAATTTT..AAAATTC)
• Location of nic site: 78..79
• Conserved sequence flanking the nic site: TTTGGTTAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 255 nt

>oriT_pF217-MCR_313k
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobH_R0Q77_RS00685_pF217-MCR_313k

• ID: 12992
• GenBank: WP_012477394
• Name: mobH_R0Q77_RS00685_pF217-MCR_313k
• UniProt ID: A0A2Z2E7B6
• Length: 1050 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1050 a.a.
• Molecular weight: 118067.47 Da
• Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (108-304)

Protein structure

• AlphaFold DB: A0A2Z2E7B6

T4CP

traD_R0Q77_RS00690_pF217-MCR_313k

• ID: 15254
• GenBank: WP_001284073
• Name: traD_R0Q77_RS00690_pF217-MCR_313k
• UniProt ID: _
• Length: 694 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 694 a.a.
• Molecular weight: 78123.91 Da
• Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (406-609)

• AAA-like domain (AAA_10) (199-249)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 199799..209039

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R0Q77_RS01080 (R0Q77_01080)	194942..195658	-	717	WP_000838219	zinc finger-like domain-containing protein	-
R0Q77_RS01085 (R0Q77_01085)	195655..195855	-	201	WP_000594930	hypothetical protein	-
R0Q77_RS01090 (R0Q77_01090)	195875..196927	-	1053	WP_326978241	hypothetical protein	-
R0Q77_RS01095 (R0Q77_01095)	197404..198279	+	876	WP_000594611	RepB family plasmid replication initiator protein	-
R0Q77_RS01100 (R0Q77_01100)	199428..199781	+	354	WP_000423602	hypothetical protein	-
R0Q77_RS01105 (R0Q77_01105)	199799..200149	+	351	WP_001447770	type IV conjugative transfer system protein TraL	traL
R0Q77_RS01110 (R0Q77_01110)	200161..200949	+	789	WP_000783153	TraE/TraK family type IV conjugative transfer system protein	traE
R0Q77_RS01115 (R0Q77_01115)	200949..202220	+	1272	WP_000592092	type-F conjugative transfer system secretin TraK	traK
R0Q77_RS01120 (R0Q77_01120)	202222..202683	+	462	WP_000521242	plasmid transfer protein HtdO	-
R0Q77_RS01125 (R0Q77_01125)	202673..204028	+	1356	WP_000351841	IncHI-type conjugal transfer protein TrhB	traB
R0Q77_RS01130 (R0Q77_01130)	204036..204518	+	483	WP_000377632	hypothetical protein	-
R0Q77_RS01135 (R0Q77_01135)	204637..205389	+	753	WP_182911723	protein-disulfide isomerase HtdT	-
R0Q77_RS01140 (R0Q77_01140)	205399..206349	+	951	WP_001022588	IncHI-type conjugal transfer lipoprotein TrhV	traV
R0Q77_RS01145 (R0Q77_01145)	206358..209039	+	2682	WP_000387412	TraC family protein	virb4
R0Q77_RS01150 (R0Q77_01150)	211588..211749	+	162	WP_001371949	hypothetical protein	-

Host bacterium

• ID: 21053
• GenBank: NZ_CP136723
• Plasmid name: pF217-MCR_313k
• Incompatibility group: IncHI2
• Plasmid size: 313339 bp
• Coordinate of oriT [Strand]: 122190..122444 [-]
• Host baterium: Citrobacter braakii strain F217

Cargo genes

• Drug resistance gene: aph(3'')-Ib, aph(6)-Id, sul1, qacE, aadA2, dfrA12, formA, catA2, mcr-9
• Virulence gene: -
• Metal resistance gene: pcoS, pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH, terW, terZ, terA, terB, terC, terD, terE, merR, merB, merD, merE
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -