Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120621
Name   oriT_pKAM621_1 in_silico
Organism   Citrobacter braakii strain KAM621
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP026383 (115794..116048 [+], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pKAM621_1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12990 GenBank   WP_012477394
Name   mobH_V0Z23_RS26090_pKAM621_1 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   15253 GenBank   WP_001284073
Name   traD_V0Z23_RS26085_pKAM621_1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2396..11633

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
V0Z23_RS25515 (KAM621c_50050) 1..876 + 876 WP_000594611 RepB family plasmid replication initiator protein -
V0Z23_RS25520 (KAM621c_50070) 2025..2378 + 354 WP_000423602 hypothetical protein -
V0Z23_RS25525 (KAM621c_50080) 2396..2746 + 351 WP_001447770 type IV conjugative transfer system protein TraL traL
V0Z23_RS25530 (KAM621c_50090) 2758..3546 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
V0Z23_RS25535 (KAM621c_50100) 3811..4815 + 1005 WP_326978217 type-F conjugative transfer system secretin TraK traK
V0Z23_RS25540 4817..5278 + 462 WP_000521242 plasmid transfer protein HtdO -
V0Z23_RS25545 (KAM621c_50120) 5268..6623 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
V0Z23_RS25550 (KAM621c_50130) 6690..7112 + 423 WP_000251249 hypothetical protein -
V0Z23_RS25555 (KAM621c_50140) 7231..7983 + 753 WP_182911723 protein-disulfide isomerase HtdT -
V0Z23_RS25560 (KAM621c_50150) 7993..8943 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
V0Z23_RS25565 (KAM621c_50160) 8952..11633 + 2682 WP_000387412 TraC family protein virb4
V0Z23_RS25570 13933..14094 + 162 WP_001371949 hypothetical protein -
V0Z23_RS25575 (KAM621c_50180) 15223..16476 + 1254 WP_001050364 ParA family protein -

Host bacterium

ID   21050 GenBank   NZ_AP026383
Plasmid name   pKAM621_1 Incompatibility group   IncHI2A
Plasmid size   246259 bp Coordinate of oriT [Strand]   115794..116048 [+]
Host baterium   Citrobacter braakii strain KAM621

Cargo genes

Drug resistance gene   tet(B)
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, arsH, arsR, arsB, arsC, rcnR/yohL, ncrC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -