Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120558
Name   oriT_p2481359-2 in_silico
Organism   Klebsiella grimontii strain 2481359
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP067382 (52493..52574 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_p2481359-2
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12950 GenBank   WP_032440569
Name   traI_JJJ10_RS29590_p2481359-2 insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   15205 GenBank   WP_032440593
Name   trbC_JJJ10_RS29455_p2481359-2 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 21451..43384

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJJ10_RS29440 (JJJ10_29435) 17214..17909 - 696 WP_254910806 RepA family replication protein -
JJJ10_RS29445 (JJJ10_29440) 18415..18666 - 252 WP_113394424 hypothetical protein -
JJJ10_RS29450 (JJJ10_29445) 18678..19211 - 534 WP_032440594 phospholipase D family protein -
JJJ10_RS29455 (JJJ10_29450) 19257..21449 - 2193 WP_032440593 F-type conjugative transfer protein TrbC -
JJJ10_RS29460 (JJJ10_29455) 21451..22572 - 1122 WP_052455213 thioredoxin fold domain-containing protein trbB
JJJ10_RS29465 (JJJ10_29460) 22553..23971 - 1419 WP_063666705 hypothetical protein trbA
JJJ10_RS29470 (JJJ10_29465) 24073..24696 - 624 WP_032440592 plasmid IncI1-type surface exclusion protein ExcA -
JJJ10_RS29475 (JJJ10_29470) 24762..26750 - 1989 WP_326937891 DotA/TraY family protein traY
JJJ10_RS29480 (JJJ10_29475) 26948..27529 - 582 WP_050597335 conjugal transfer protein TraX -
JJJ10_RS29485 (JJJ10_29480) 27526..28722 - 1197 WP_032440590 conjugal transfer protein TraW traW
JJJ10_RS29490 (JJJ10_29485) 28732..31779 - 3048 WP_032440589 conjugal transfer protein traU
JJJ10_RS29495 (JJJ10_29490) 31789..32352 - 564 WP_045325070 hypothetical protein traT
JJJ10_RS29500 (JJJ10_29495) 32396..32797 - 402 WP_032440588 DUF6750 family protein traR
JJJ10_RS29505 (JJJ10_29500) 32853..33386 - 534 WP_032440587 conjugal transfer protein TraQ traQ
JJJ10_RS29510 (JJJ10_29505) 33396..34115 - 720 WP_050597333 conjugal transfer protein TraP traP
JJJ10_RS29515 (JJJ10_29510) 34122..35462 - 1341 WP_032440586 conjugal transfer protein TraO traO
JJJ10_RS29520 (JJJ10_29515) 35466..36569 - 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
JJJ10_RS29525 (JJJ10_29520) 36580..37248 - 669 WP_032440585 DotI/IcmL/TraM family protein traM
JJJ10_RS29530 (JJJ10_29525) 37320..37673 - 354 WP_032440584 hypothetical protein traL
JJJ10_RS29535 (JJJ10_29530) 37707..41120 - 3414 WP_052145516 LPD7 domain-containing protein -
JJJ10_RS29540 (JJJ10_29535) 41138..41404 - 267 WP_032440577 IcmT/TraK family protein traK
JJJ10_RS29545 (JJJ10_29540) 41401..42588 - 1188 WP_077255878 plasmid transfer ATPase TraJ virB11
JJJ10_RS29550 (JJJ10_29545) 42566..43384 - 819 WP_032440576 type IV secretory system conjugative DNA transfer family protein traI
JJJ10_RS29555 (JJJ10_29550) 43381..43845 - 465 WP_225546581 DotD/TraH family lipoprotein -
JJJ10_RS29560 (JJJ10_29555) 43871..44350 - 480 WP_032440574 lytic transglycosylase domain-containing protein -
JJJ10_RS29565 (JJJ10_29560) 45082..45309 - 228 WP_032440573 hypothetical protein -
JJJ10_RS29570 (JJJ10_29565) 45919..46761 + 843 WP_032440572 helix-turn-helix domain-containing protein -
JJJ10_RS29575 (JJJ10_29570) 46821..47750 + 930 WP_032440581 glycosyltransferase family 9 protein -
JJJ10_RS29580 (JJJ10_29575) 47743..48237 + 495 WP_032440571 molybdopterin-dependent oxidoreductase -

Host bacterium

ID   20987 GenBank   NZ_CP067382
Plasmid name   p2481359-2 Incompatibility group   IncFII
Plasmid size   75780 bp Coordinate of oriT [Strand]   52493..52574 [-]
Host baterium   Klebsiella grimontii strain 2481359

Cargo genes

Drug resistance gene   blaLAP-2, ARR-3, cmlA1, blaOXA-10, ant(3'')-Ia, dfrA14
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -