Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120554
Name   oriT_pRHBSTW-00866_2 in_silico
Organism   Klebsiella grimontii strain RHBSTW-00866
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP055310 (188203..188486 [-], 284 nt)
oriT length   284 nt
IRs (inverted repeats)      183..188, 190..195  (AAAAGT..ACTTTT)
 45..50, 55..60  (TTTTAA..TTAAAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 284 nt

>oriT_pRHBSTW-00866_2
GTTATTGCCGCTTAATGCCGATAACGATTCGGGCTTTGCTATTTTTTTAAGCGATTAAAATTTCGTTAGCAAGCGCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTCAAAAAGTGTTACAAGTAAGGCCAATGGATGAATGGTTAGTTTTAAGACTCAAATAGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCATTCAAAAACCAAACTTTAATTTCATGTAGAAGATTAGTACAATTGTATTGACGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12947 GenBank   WP_223203175
Name   mobH_HV306_RS30700_pRHBSTW-00866_2 insolico UniProt ID   _
Length   988 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 988 a.a.        Molecular weight: 110156.87 Da        Isoelectric Point: 4.5476

>WP_223203175.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MAELMMKDVSSLSPFAQIIGDQKYTVPDHPNLEVLKFIEYPTRPAGIQTFNEQSILSLYRDKLHSISMML
AISDGDIREDAFTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLLSHSLEVAMISLKNANHSELRPIG
YQDEEVIRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLNETLTWAPSSQSLLDWARENNVVEYEIHWRKR
IHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDGNDFLSKCVRTSDYYSTGTDLNVL
RDPIMGLRSNDAAARAIGTIKHNFTSININNYKSKPMHIIIVNGEVYLNENAFLDFVLSDFAAHKFNFPQ
GDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENEIASIFRNGIGKLEFYNLLKLRWIGLIFDSYK
IPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVSDAIEDTPQYGLKLVNGPDADSVN
GKSSENTEAITESVEAAGTDKATDIIDPQVTTPTDTAGTGGLDEKERVEEHEEQSQMRLVEQLHEMLLSA
PLPHNAVINIDSVPYLNLDAAIALIPGIDEAAFCDGPFFQLTYRDGSLDGMWIVRDVNNLRLIQLGDNCA
GIQVSTSEPRTTSTLKSLFDTSMYQPLDIPESPSLNEAVSPPQTPLDLPPARLNTPQAVEANSVAEQINA
HSEPETVTASEYEQYGHLLEETLDSDSEAYSDFIVADNSVVEPPQPEAKNSDVAEFHDETDQVVAPGKLN
DPEDTREPPAPNTTGKEVILTSPVSSQDPIKSEPAGEKANHLSPALARLFAVNTQAEKKHENPPVTTPVK
EAFNRSNSATVQAPIAVDQPGAEEKETLEDFTLLSGGEVTELEYVEIATMLHHILTKLSGSFKRKRKNRF
MVLTQNTFFLTQSCVEKYGTQLNAPELFNQLPQYQVTSGAIVNTKCFAFNMPTLVAASDRAKVDIELIIN
TLKDVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(83-278)


  Protein structure



No available structure.




T4CP

ID   15202 GenBank   WP_015063005
Name   traD_HV306_RS30705_pRHBSTW-00866_2 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77900.52 Da        Isoelectric Point: 8.5005

>WP_015063005.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MAKSKRTNLHAQENFYRPILEYRSASVLLICAIIMLFMGFRSDGLNIAPIILYTAAFMLLVCLYRCKTAY
PYLMAHWRVFQRQIMFISLKSLRSINKSNFFSNERKYRQLVQEYKKSNRQVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFALNPIARHFEAMARSMGGSNAIFAVDKRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDADWRQSLMDEAAELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMAILNLTVKVLECCYARH
YGPDVWMEKVKYSSNDTLQVRFKRLVEWFSAHFLNYEGAEPIDWIDTVGRLVDYSLSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNSLTSREIVTSDGIFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNSDDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSENSMRPNTTLLEMFLKPAGKVTLRQNVGLDYLNKHLRKLH

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(505-627)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2799..11967

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV306_RS29685 (HV306_29700) 1..876 + 876 WP_015063098 RepB family plasmid replication initiator protein -
HV306_RS29690 (HV306_29705) 1919..2104 + 186 WP_032610388 hypothetical protein -
HV306_RS29695 (HV306_29710) 2394..2747 + 354 WP_015063100 hypothetical protein -
HV306_RS29700 (HV306_29715) 2799..3116 + 318 WP_022652151 type IV conjugative transfer system protein TraL traL
HV306_RS29705 (HV306_29720) 3128..3913 + 786 WP_022652152 TraE/TraK family type IV conjugative transfer system protein traE
HV306_RS29710 (HV306_29725) 3916..5154 + 1239 WP_015063103 type-F conjugative transfer system secretin TraK traK
HV306_RS29715 (HV306_29730) 5156..5596 + 441 WP_015063104 plasmid transfer protein HtdO -
HV306_RS29720 (HV306_29735) 5586..6944 + 1359 WP_022652153 IncHI-type conjugal transfer protein TrhB traB
HV306_RS29725 (HV306_29740) 6953..7459 + 507 WP_015063106 hypothetical protein -
HV306_RS29730 (HV306_29745) 7565..8317 + 753 WP_185757620 protein-disulfide isomerase HtdT -
HV306_RS29735 (HV306_29750) 8327..9277 + 951 WP_015063108 TraV family lipoprotein traV
HV306_RS29740 (HV306_29755) 9286..11967 + 2682 WP_015063109 IncHI-type conjugal transfer ATPase TrhC virb4
HV306_RS29745 (HV306_29760) 12827..13231 + 405 WP_003031976 transposase -
HV306_RS29750 (HV306_29765) 13228..13575 + 348 WP_000612626 IS66 family insertion sequence element accessory protein TnpB -
HV306_RS29755 (HV306_29770) 13624..15162 + 1539 WP_004201219 IS66-like element ISKpn24 family transposase -

Host bacterium

ID   20983 GenBank   NZ_CP055310
Plasmid name   pRHBSTW-00866_2 Incompatibility group   IncHI1A
Plasmid size   269564 bp Coordinate of oriT [Strand]   188203..188486 [-]
Host baterium   Klebsiella grimontii strain RHBSTW-00866

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE, terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -