Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120547
Name   oriT_pRHBSTW-00555_2 in_silico
Organism   Klebsiella grimontii strain RHBSTW-00555
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP055316 (194123..194406 [-], 284 nt)
oriT length   284 nt
IRs (inverted repeats)      183..188, 190..195  (AAAAGT..ACTTTT)
 45..50, 55..60  (TTTTAA..TTAAAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 284 nt

>oriT_pRHBSTW-00555_2
GTTATTGCCGCTTAATGCCGATAACGATTCGGGCTTTGCTATTTTTTTAAGCGATTAAAATTTCGTTAGCAAGCGCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTCAAAAAGTGTTACAAGTAAGGCCAATGGATGAATGGTTAGTTTTAAGACTCAAATAGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCATTCAAAAACCAAACTTTAATTTCATGTAGAAGATTAGTACAATTGTATTGACGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12944 GenBank   WP_223203175
Name   mobH_HV234_RS29060_pRHBSTW-00555_2 insolico UniProt ID   _
Length   988 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 988 a.a.        Molecular weight: 110156.87 Da        Isoelectric Point: 4.5476

>WP_223203175.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MAELMMKDVSSLSPFAQIIGDQKYTVPDHPNLEVLKFIEYPTRPAGIQTFNEQSILSLYRDKLHSISMML
AISDGDIREDAFTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLLSHSLEVAMISLKNANHSELRPIG
YQDEEVIRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLNETLTWAPSSQSLLDWARENNVVEYEIHWRKR
IHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDGNDFLSKCVRTSDYYSTGTDLNVL
RDPIMGLRSNDAAARAIGTIKHNFTSININNYKSKPMHIIIVNGEVYLNENAFLDFVLSDFAAHKFNFPQ
GDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENEIASIFRNGIGKLEFYNLLKLRWIGLIFDSYK
IPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVSDAIEDTPQYGLKLVNGPDADSVN
GKSSENTEAITESVEAAGTDKATDIIDPQVTTPTDTAGTGGLDEKERVEEHEEQSQMRLVEQLHEMLLSA
PLPHNAVINIDSVPYLNLDAAIALIPGIDEAAFCDGPFFQLTYRDGSLDGMWIVRDVNNLRLIQLGDNCA
GIQVSTSEPRTTSTLKSLFDTSMYQPLDIPESPSLNEAVSPPQTPLDLPPARLNTPQAVEANSVAEQINA
HSEPETVTASEYEQYGHLLEETLDSDSEAYSDFIVADNSVVEPPQPEAKNSDVAEFHDETDQVVAPGKLN
DPEDTREPPAPNTTGKEVILTSPVSSQDPIKSEPAGEKANHLSPALARLFAVNTQAEKKHENPPVTTPVK
EAFNRSNSATVQAPIAVDQPGAEEKETLEDFTLLSGGEVTELEYVEIATMLHHILTKLSGSFKRKRKNRF
MVLTQNTFFLTQSCVEKYGTQLNAPELFNQLPQYQVTSGAIVNTKCFAFNMPTLVAASDRAKVDIELIIN
TLKDVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(83-278)


  Protein structure



No available structure.




T4CP

ID   15199 GenBank   WP_015063005
Name   traD_HV234_RS29065_pRHBSTW-00555_2 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77900.52 Da        Isoelectric Point: 8.5005

>WP_015063005.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MAKSKRTNLHAQENFYRPILEYRSASVLLICAIIMLFMGFRSDGLNIAPIILYTAAFMLLVCLYRCKTAY
PYLMAHWRVFQRQIMFISLKSLRSINKSNFFSNERKYRQLVQEYKKSNRQVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFALNPIARHFEAMARSMGGSNAIFAVDKRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDADWRQSLMDEAAELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMAILNLTVKVLECCYARH
YGPDVWMEKVKYSSNDTLQVRFKRLVEWFSAHFLNYEGAEPIDWIDTVGRLVDYSLSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNSLTSREIVTSDGIFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNSDDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSENSMRPNTTLLEMFLKPAGKVTLRQNVGLDYLNKHLRKLH

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(505-627)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2799..11967

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV234_RS28005 (HV234_28010) 1..876 + 876 WP_015063098 RepB family plasmid replication initiator protein -
HV234_RS28010 (HV234_28015) 1919..2104 + 186 WP_032610388 hypothetical protein -
HV234_RS28015 (HV234_28020) 2394..2747 + 354 WP_015063100 hypothetical protein -
HV234_RS28020 (HV234_28025) 2799..3116 + 318 WP_022652151 type IV conjugative transfer system protein TraL traL
HV234_RS28025 (HV234_28030) 3128..3913 + 786 WP_022652152 TraE/TraK family type IV conjugative transfer system protein traE
HV234_RS28030 (HV234_28035) 3916..5154 + 1239 WP_015063103 type-F conjugative transfer system secretin TraK traK
HV234_RS28035 (HV234_28040) 5156..5596 + 441 WP_015063104 plasmid transfer protein HtdO -
HV234_RS28040 (HV234_28045) 5586..6944 + 1359 WP_022652153 IncHI-type conjugal transfer protein TrhB traB
HV234_RS28045 (HV234_28050) 6953..7459 + 507 WP_015063106 hypothetical protein -
HV234_RS28050 (HV234_28055) 7565..8317 + 753 WP_185757620 protein-disulfide isomerase HtdT -
HV234_RS28055 (HV234_28060) 8327..9277 + 951 WP_015063108 TraV family lipoprotein traV
HV234_RS28060 (HV234_28065) 9286..11967 + 2682 WP_015063109 IncHI-type conjugal transfer ATPase TrhC virb4
HV234_RS28065 (HV234_28070) 12827..13231 + 405 WP_003031976 transposase -
HV234_RS28070 (HV234_28075) 13228..13575 + 348 WP_000612626 IS66 family insertion sequence element accessory protein TnpB -
HV234_RS28075 (HV234_28080) 13624..15162 + 1539 WP_004201219 IS66-like element ISKpn24 family transposase -

Host bacterium

ID   20976 GenBank   NZ_CP055316
Plasmid name   pRHBSTW-00555_2 Incompatibility group   IncHI1A
Plasmid size   273344 bp Coordinate of oriT [Strand]   194123..194406 [-]
Host baterium   Klebsiella grimontii strain RHBSTW-00555

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE, arsC, arsB, arsR, arsH, terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -