Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 120543
• Name: oriT_pIMPIncH12_334kb
• Organism: Klebsiella aerogenes strain KA_P10_L5_03.19
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP044215 (205408..205662 [-], 255 nt)
• oriT length: 255 nt
• IRs (inverted repeats): 153..158, 160..165 (AAAAGT..ACTTTT) 122..130, 135..143 (TTAAGGCTT..AAGCCTTAA) 49..55, 60..66 (GAATTTT..AAAATTC)
• Location of nic site: 78..79
• Conserved sequence flanking the nic site: TTTGGTTAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 255 nt

>oriT_pIMPIncH12_334kb
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobH_F6O44_RS25645_pIMPIncH12_334kb

• ID: 12942
• GenBank: WP_012477394
• Name: mobH_F6O44_RS25645_pIMPIncH12_334kb
• UniProt ID: A0A2Z2E7B6
• Length: 1050 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1050 a.a.
• Molecular weight: 118067.47 Da
• Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (108-304)

Protein structure

• AlphaFold DB: A0A2Z2E7B6

T4CP

traD_F6O44_RS25650_pIMPIncH12_334kb

• ID: 15198
• GenBank: WP_001284073
• Name: traD_F6O44_RS25650_pIMPIncH12_334kb
• UniProt ID: _
• Length: 694 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 694 a.a.
• Molecular weight: 78123.91 Da
• Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (406-609)

• AAA-like domain (AAA_10) (199-249)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33922..43162

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6O44_RS24690 (F6O44_24695)	29065..29781	-	717	WP_000838219	zinc finger-like domain-containing protein	-
F6O44_RS24695 (F6O44_24700)	29778..29978	-	201	WP_000594930	hypothetical protein	-
F6O44_RS24700 (F6O44_24705)	29998..31050	-	1053	WP_001065778	hypothetical protein	-
F6O44_RS24705 (F6O44_24710)	31527..32402	+	876	WP_000594611	RepB family plasmid replication initiator protein	-
F6O44_RS24715 (F6O44_24720)	33551..33904	+	354	WP_000423602	hypothetical protein	-
F6O44_RS24720 (F6O44_24725)	33922..34272	+	351	WP_001447770	type IV conjugative transfer system protein TraL	traL
F6O44_RS24725 (F6O44_24730)	34284..35072	+	789	WP_000783153	TraE/TraK family type IV conjugative transfer system protein	traE
F6O44_RS24730 (F6O44_24735)	35072..36343	+	1272	WP_000592092	type-F conjugative transfer system secretin TraK	traK
F6O44_RS24735 (F6O44_24740)	36345..36806	+	462	WP_000521242	plasmid transfer protein HtdO	-
F6O44_RS24740 (F6O44_24745)	36796..38151	+	1356	WP_000351841	IncHI-type conjugal transfer protein TrhB	traB
F6O44_RS24745 (F6O44_24750)	38159..38641	+	483	WP_000377632	hypothetical protein	-
F6O44_RS24750 (F6O44_24755)	38760..39512	+	753	WP_182911723	protein-disulfide isomerase HtdT	-
F6O44_RS24755 (F6O44_24760)	39522..40472	+	951	WP_151085590	IncHI-type conjugal transfer lipoprotein TrhV	traV
F6O44_RS24760 (F6O44_24765)	40481..43162	+	2682	WP_151085591	TraC family protein	virb4
F6O44_RS26575	45711..45872	+	162	WP_001371949	hypothetical protein	-

Host bacterium

• ID: 20972
• GenBank: NZ_CP044215
• Plasmid name: pIMPIncH12_334kb
• Incompatibility group: IncHI2
• Plasmid size: 333880 bp
• Coordinate of oriT [Strand]: 205408..205662 [-]
• Host baterium: Klebsiella aerogenes strain KA_P10_L5_03.19

Cargo genes

• Drug resistance gene: blaTEM-1B, catA2, mcr-9, aph(6)-Id, aph(3'')-Ib, dfrA19, sul1, qacE, blaIMP-70, qnrA1, aac(6')-IIc, ere(A), blaSHV-12
• Virulence gene: -
• Metal resistance gene: terW, terZ, terA, terB, terC, terD, terE, pbrA, merE, merD, merA, merC, merP, merT, merR, arsH, arsR, arsB, arsC, rcnR/yohL, ncrC, pcoE, pcoS
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -