Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   120537
Name   oriT_p11A17009_A_KPC in_silico
Organism   Klebsiella oxytoca strain 11A17CRGN009
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OQ821087 (86423..86528 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_p11A17009_A_KPC
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   12937 GenBank   WP_004187323
Name   traI_SLH58_RS00555_p11A17009_A_KPC insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP


ID   15194 GenBank   WP_004187443
Name   t4cp2_SLH58_RS00020_p11A17009_A_KPC insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79519.87 Da        Isoelectric Point: 4.8309

>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 89324..108517

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SLH58_RS00530 (CBKDIOPN_00106) 84408..84719 + 312 WP_004187333 hypothetical protein -
SLH58_RS00535 (CBKDIOPN_00107) 84891..85388 + 498 WP_260466953 hypothetical protein -
SLH58_RS00540 (CBKDIOPN_00108) 85458..85598 + 141 WP_015062823 hypothetical protein -
SLH58_RS00545 (CBKDIOPN_00109) 85890..86285 - 396 WP_019725163 hypothetical protein -
SLH58_RS00550 (CBKDIOPN_00110) 86284..86847 + 564 WP_020277892 plasmid mobilization protein MobA -
SLH58_RS00555 (CBKDIOPN_00111) 86834..88813 + 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
SLH58_RS00560 (CBKDIOPN_00112) 88827..89327 + 501 WP_004187320 DotD/TraH family lipoprotein -
SLH58_RS00565 (CBKDIOPN_00113) 89324..90103 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
SLH58_RS00570 (CBKDIOPN_00114) 90114..91277 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
SLH58_RS00575 (CBKDIOPN_00115) 91267..91527 + 261 WP_004187310 IcmT/TraK family protein traK
SLH58_RS00580 (CBKDIOPN_00116) 91552..94854 + 3303 WP_319382161 LPD7 domain-containing protein -
SLH58_RS00585 (CBKDIOPN_00117) 94820..95332 + 513 WP_011091071 hypothetical protein traL
SLH58_RS00590 (CBKDIOPN_00118) 95333..95545 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
SLH58_RS00595 (CBKDIOPN_00119) 95517..95927 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
SLH58_RS00600 (CBKDIOPN_00120) 95926..96708 + 783 WP_011091073 DotI/IcmL family type IV secretion protein traM
SLH58_RS00605 (CBKDIOPN_00121) 96717..97868 + 1152 WP_011091074 DotH/IcmK family type IV secretion protein traN
SLH58_RS00610 (CBKDIOPN_00122) 97880..99229 + 1350 WP_011091075 conjugal transfer protein TraO traO
SLH58_RS00615 (CBKDIOPN_00123) 99241..99945 + 705 WP_031942491 conjugal transfer protein TraP traP
SLH58_RS00620 (CBKDIOPN_00124) 99969..100499 + 531 WP_011091077 conjugal transfer protein TraQ traQ
SLH58_RS00625 (CBKDIOPN_00125) 100516..100905 + 390 WP_011091078 DUF6750 family protein traR
SLH58_RS00630 (CBKDIOPN_00126) 100951..101445 + 495 WP_011091080 hypothetical protein -
SLH58_RS00635 (CBKDIOPN_00127) 101442..104492 + 3051 WP_319382162 conjugal transfer protein traU
SLH58_RS00640 (CBKDIOPN_00128) 104489..105697 + 1209 WP_011091082 conjugal transfer protein TraW traW
SLH58_RS00645 (CBKDIOPN_00129) 105694..106344 + 651 WP_004187488 hypothetical protein -
SLH58_RS00650 106337..108517 + 2181 WP_004187492 DotA/TraY family protein traY
SLH58_RS00655 (CBKDIOPN_00132) 108520..109173 + 654 WP_004206935 hypothetical protein -
SLH58_RS00660 (CBKDIOPN_00133) 109247..109477 + 231 WP_004187496 IncL/M type plasmid replication protein RepC -


Host bacterium


ID   20966 GenBank   NZ_OQ821087
Plasmid name   p11A17009_A_KPC Incompatibility group   IncL/M
Plasmid size   109773 bp Coordinate of oriT [Strand]   86423..86528 [+]
Host baterium   Klebsiella oxytoca strain 11A17CRGN009

Cargo genes


Drug resistance gene   sul1, qnrB2, blaKPC-3, tet(A), ant(2'')-Ia, blaSHV-30
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -