Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   120531
Name   oriT_p01A15005_B_KPC in_silico
Organism   Klebsiella oxytoca strain 01A15CRGN005
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OQ821011 (46697..46745 [-], 49 nt)
oriT length   49 nt
IRs (inverted repeats)      6..13, 16..23  (GCAAAATT..AATTTTGC)
Location of nic site      32..33
Conserved sequence flanking the
  nic site  
 
 GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 49 nt

>oriT_p01A15005_B_KPC
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   12931 GenBank   WP_319381996
Name   traI_SLH58_RS00510_p01A15005_B_KPC insolico UniProt ID   _
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190170.84 Da        Isoelectric Point: 6.2774

>WP_319381996.1 conjugative transfer relaxase/helicase TraI [Klebsiella oxytoca]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMEERWQGKGAEALGLDGKAVDKAVFTELLKGKLPDGSDLTRM
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLNDAHNQAVTVAVKQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQLHTHAVVINATQNGDKWQSLGTDTVGKTGFIENVYANQTAFGKLYREALKPLVE
KLGYETKVVGKHGMWEMKDVPVEPFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADAHAAELARAPAAPVKTDGPDIADVVTRAIAGLSDRKVQFTYADVLARTVGQLE
AKPGVFELARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEIQRQNHVSVTPDAGVVREKP
FSDAVSVMAQDRPAMGIVAGQGGAAGQRERVAELTLMAREQGRDVHILAADNRSREFLAGDARLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGKQATADIISEPDKSARYSRLAQEFAASVREGQESVAQISGTREQNVLNNVIRDTLKNEGALGNRD
MTVTALTPVWLDSKSRGVRDYYREGMVMERWDPEARKHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRTDKLPVAEGERLAVLGKIPDTRLKGGESVTVLKAEAGQLTVQRPGQKTTQTLSVGSSPFDGI
KVGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQDSARTTEKLARHTAFSVVSE
QLKVRSGETDLEAAIAQQKAGLHTPAEQAIHLSVPLLESNSLTFTRPQLLATALETGGGKVPMKDIEATI
QAQIKAGSLLNVPVASGHGNDLLISRQTWDAEKSVLTRVLEGKDAVAPLMDRVPGSLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLH
DTQLQQRNGQTPDFSNTLFLLDESSMVGLADTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDVSRALSTIEQVTPEQVPRKDGAWVPENSVTEFTPAQEKAIQ
DALKEGKEIPPGTPVSLHDAIVKDYTGRTPAAQASTLIITHLNADRRALNGLIHDARVQNGEVSKEEVTL
PVLVTSNIRDGELRKLSTWSEHQGAVALLDNTYQRIESVDKENQLITLKDNEGNIRLLSPREASAEGVTL
YRPEHITVSQGDRMRFSKSDPERGYVANSVWEVKAFSGDSVTLSDGKTTRTLNPKADEAQRHIDLAYAIT
AHGAQGASEPFAIALEGVEGRRKALVSFESAYVALSRMKQHAQVYTDNREGWTTAVSRSQEKASAHDVLE
PQNARAVRNAGQLYGRAQPLDETAAGRAALQQSGLARGRSPGKFISPGKKYPQPHVALPAFDKNGKEAGI
WLSPLTDRDGRLEAIGGEGRIMGDETARFVALQSSRNGESLLAGNMGEGVRIARDNPDSGVIVRLAGDDR
PWNPEAITGGRIWADPLPNPPLPDTGTDIPLPPEVLAQRAAEEAQRREMEKQAEQATREVAGEEKKAGEP
GERIKEVIGDVIRGLERDRPGEEKTRLPDDPQTRRQEAAIQQVASESLQRERLQQMERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

(577-629)

(1433-1555)

(635-714)

(10-284)

(971-1158)


  Protein structure



No available structure.




Auxiliary protein


ID   7266 GenBank   WP_032426796
Name   WP_032426796_p01A15005_B_KPC insolico UniProt ID   _
Length   130 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 130 a.a.        Molecular weight: 15036.10 Da        Isoelectric Point: 4.5326

>WP_032426796.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacterales]
MGKVQAYPSDEVCEKINAIVEKRRMEGAKEKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNRAL
LESVVKTQFSVNKVLGIECLSPHVKDDPRWQWKGMVQNIQEDVQEVMRTFFPDEQEEADE

  Protein domains


Predicted by InterproScan.

(1-125)


  Protein structure



No available structure.




T4CP


ID   15187 GenBank   WP_319381995
Name   traD_SLH58_RS00505_p01A15005_B_KPC insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 85519.29 Da        Isoelectric Point: 4.8808

>WP_319381995.1 type IV conjugative transfer system coupling protein TraD [Klebsiella oxytoca]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRTLDTRVDARLSALLEAREAEGSLARALFTPDAPEPEQSDADSKPSPGQPEPESAP
VSPAPVKSASTAETLAAQPSVKASEAPQAKVTTVPLTRPAPAATPAAASATASSAAATAAATGGTEQALA
QQPADTGQEMMPAGMNEDGEIEDMQAYDAWLADEQTQREMQRREEVNINHSHRRDEQDDIEIGGNF

  Protein domains


Predicted by InterproScan.

(32-128)

(172-560)

  Protein structure



No available structure.



ID   15188 GenBank   WP_004152620
Name   traD_SLH58_RS00595_p01A15005_B_KPC insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85871.84 Da        Isoelectric Point: 5.0101

>WP_004152620.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

(32-128)

(172-560)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 49163..72212

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SLH58_RS00340 45202..45378 - 177 WP_319382015 hypothetical protein -
SLH58_RS00345 (KEGJGPDJ_00059) 45565..46107 + 543 WP_319382016 antirestriction protein -
SLH58_RS00350 (KEGJGPDJ_00060) 46130..46552 - 423 WP_239605204 transglycosylase SLT domain-containing protein -
SLH58_RS00355 (KEGJGPDJ_00061) 47058..47450 + 393 WP_032426796 conjugal transfer relaxosome DNA-binding protein TraM -
SLH58_RS00360 (KEGJGPDJ_00062) 47688..48380 + 693 WP_032426795 conjugal transfer protein TrbJ -
SLH58_RS00365 48556..48720 + 165 WP_032426810 TraY domain-containing protein -
SLH58_RS00370 (KEGJGPDJ_00063) 48784..49149 + 366 WP_064381359 type IV conjugative transfer system pilin TraA -
SLH58_RS00375 (KEGJGPDJ_00064) 49163..49468 + 306 WP_020323523 type IV conjugative transfer system protein TraL traL
SLH58_RS00380 (KEGJGPDJ_00065) 49488..50054 + 567 WP_064373744 type IV conjugative transfer system protein TraE traE
SLH58_RS00385 (KEGJGPDJ_00066) 50041..50781 + 741 WP_319382017 type-F conjugative transfer system secretin TraK traK
SLH58_RS00390 (KEGJGPDJ_00067) 50781..52199 + 1419 WP_319382018 F-type conjugal transfer pilus assembly protein TraB traB
SLH58_RS00395 (KEGJGPDJ_00068) 52192..52788 + 597 WP_319382019 conjugal transfer protein TraP -
SLH58_RS00400 52856..52993 + 138 Protein_79 conjugal transfer protein TraP -
SLH58_RS00405 (KEGJGPDJ_00070) 53016..53582 + 567 WP_319382020 type IV conjugative transfer system lipoprotein TraV traV
SLH58_RS00410 (KEGJGPDJ_00071) 53699..54103 + 405 WP_319382021 hypothetical protein -
SLH58_RS00415 (KEGJGPDJ_00072) 54132..54425 + 294 WP_319382022 hypothetical protein -
SLH58_RS00420 (KEGJGPDJ_00073) 54449..54673 + 225 WP_064381372 hypothetical protein -
SLH58_RS00425 (KEGJGPDJ_00074) 54670..55074 + 405 WP_319382023 hypothetical protein -
SLH58_RS00430 (KEGJGPDJ_00075) 55205..57844 + 2640 WP_319382024 type IV secretion system protein TraC virb4
SLH58_RS00435 (KEGJGPDJ_00076) 57877..58278 + 402 WP_319382034 type-F conjugative transfer system protein TrbI -
SLH58_RS00440 (KEGJGPDJ_00077) 58275..58901 + 627 WP_319382025 type-F conjugative transfer system protein TraW traW
SLH58_RS00445 (KEGJGPDJ_00078) 58921..59904 + 984 WP_319382035 conjugal transfer pilus assembly protein TraU traU
SLH58_RS00450 (KEGJGPDJ_00079) 59917..60534 + 618 WP_319382026 type-F conjugative transfer system pilin assembly protein TrbC trbC
SLH58_RS00455 (KEGJGPDJ_00080) 60531..62486 + 1956 WP_319382027 type-F conjugative transfer system mating-pair stabilization protein TraN traN
SLH58_RS00460 (KEGJGPDJ_00081) 62524..62805 + 282 WP_319382028 hypothetical protein -
SLH58_RS00465 (KEGJGPDJ_00082) 62795..63022 + 228 WP_064174417 conjugal transfer protein TrbE -
SLH58_RS00470 (KEGJGPDJ_00083) 63033..63359 + 327 WP_135800727 hypothetical protein -
SLH58_RS00475 (KEGJGPDJ_00084) 63378..64130 + 753 WP_319382029 type-F conjugative transfer system pilin assembly protein TraF traF
SLH58_RS00480 (KEGJGPDJ_00085) 64141..64380 + 240 WP_319382030 type-F conjugative transfer system pilin chaperone TraQ -
SLH58_RS00485 (KEGJGPDJ_00086) 64352..64927 + 576 WP_319382031 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
SLH58_RS00490 (KEGJGPDJ_00087) 64914..66284 + 1371 WP_319382032 conjugal transfer pilus assembly protein TraH traH
SLH58_RS00495 (KEGJGPDJ_00088) 66284..69127 + 2844 WP_319382033 conjugal transfer mating-pair stabilization protein TraG traG
SLH58_RS00500 (KEGJGPDJ_00089) 69138..69674 + 537 WP_301718420 hypothetical protein -
SLH58_RS00505 (KEGJGPDJ_00090) 69912..72212 + 2301 WP_319381995 type IV conjugative transfer system coupling protein TraD virb4


Host bacterium


ID   20960 GenBank   NZ_OQ821011
Plasmid name   p01A15005_B_KPC Incompatibility group   IncR
Plasmid size   134488 bp Coordinate of oriT [Strand]   46697..46745 [-]
Host baterium   Klebsiella oxytoca strain 01A15CRGN005

Cargo genes


Drug resistance gene   blaKPC-2, blaOXA-9, blaTEM-1A, aph(6)-Id, aph(3'')-Ib
Virulence gene   -
Metal resistance gene   merE, merD, merA
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -