Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120472 |
| Name | oriT_p24A19035_A_KPC  |
| Organism | Enterobacter hormaechei subsp. xiangfangensis strain 24A19CPO035 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OQ821157 (48847..48952 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_p24A19035_A_KPC
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12893 | GenBank | WP_004187323 |
| Name | traI_SLH58_RS00335_p24A19035_A_KPC |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 15142 | GenBank | WP_038874906 |
| Name | t4cp2_SLH58_RS00060_p24A19035_A_KPC |
UniProt ID | _ |
| Length | 701 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 701 a.a. Molecular weight: 80068.49 Da Isoelectric Point: 4.8705
>WP_038874906.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAESNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIGVVSEFG
K
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAESNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIGVVSEFG
K
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 51748..71076
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SLH58_RS00315 (MGPICNIJ_00061) | 46834..47145 | + | 312 | WP_004187333 | hypothetical protein | - |
| SLH58_RS00320 (MGPICNIJ_00062) | 47277..47813 | + | 537 | WP_011091066 | hypothetical protein | - |
| SLH58_RS00325 (MGPICNIJ_00064) | 48314..48709 | - | 396 | WP_020805633 | hypothetical protein | - |
| SLH58_RS00330 | 48708..49271 | + | 564 | WP_319382137 | plasmid mobilization protein MobA | - |
| SLH58_RS00335 (MGPICNIJ_00066) | 49258..51237 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| SLH58_RS00340 (MGPICNIJ_00067) | 51251..51751 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| SLH58_RS00345 (MGPICNIJ_00068) | 51748..52527 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| SLH58_RS00350 (MGPICNIJ_00069) | 52538..53701 | + | 1164 | WP_020805653 | plasmid transfer ATPase TraJ | virB11 |
| SLH58_RS00355 (MGPICNIJ_00070) | 53691..53951 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| SLH58_RS00360 (MGPICNIJ_00071) | 53976..57413 | + | 3438 | WP_319382138 | LPD7 domain-containing protein | - |
| SLH58_RS00365 (MGPICNIJ_00072) | 57337..57891 | + | 555 | WP_011154467 | hypothetical protein | traL |
| SLH58_RS00370 (MGPICNIJ_00073) | 57892..58089 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| SLH58_RS00375 (MGPICNIJ_00074) | 58076..58486 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| SLH58_RS00380 (MGPICNIJ_00075) | 58485..59267 | + | 783 | WP_004206929 | DotI/IcmL family type IV secretion protein | traM |
| SLH58_RS00385 (MGPICNIJ_00076) | 59303..60427 | + | 1125 | WP_319382139 | DotH/IcmK family type IV secretion protein | traN |
| SLH58_RS00390 (MGPICNIJ_00077) | 60439..61788 | + | 1350 | WP_020805691 | conjugal transfer protein TraO | traO |
| SLH58_RS00395 (MGPICNIJ_00078) | 61800..62504 | + | 705 | WP_020805696 | conjugal transfer protein TraP | traP |
| SLH58_RS00400 (MGPICNIJ_00079) | 62528..63058 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| SLH58_RS00405 (MGPICNIJ_00080) | 63075..63464 | + | 390 | WP_020805690 | DUF6750 family protein | traR |
| SLH58_RS00410 (MGPICNIJ_00081) | 63510..64004 | + | 495 | WP_223294957 | hypothetical protein | - |
| SLH58_RS00415 (MGPICNIJ_00082) | 64001..67051 | + | 3051 | WP_020805687 | ATPase AAA | traU |
| SLH58_RS00420 (MGPICNIJ_00083) | 67081..68256 | + | 1176 | WP_167577398 | conjugal transfer protein TraW | traW |
| SLH58_RS00425 | 68532..68903 | + | 372 | WP_319382228 | hypothetical protein | - |
| SLH58_RS00430 (MGPICNIJ_00085) | 68896..71076 | + | 2181 | WP_032426815 | DotA/TraY family protein | traY |
| SLH58_RS00435 (MGPICNIJ_00086) | 71079..71732 | + | 654 | WP_020805693 | hypothetical protein | - |
| SLH58_RS00440 (MGPICNIJ_00087) | 71794..72036 | + | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 20901 | GenBank | NZ_OQ821157 |
| Plasmid name | p24A19035_A_KPC | Incompatibility group | IncL/M |
| Plasmid size | 72332 bp | Coordinate of oriT [Strand] | 48847..48952 [+] |
| Host baterium | Enterobacter hormaechei subsp. xiangfangensis strain 24A19CPO035 |
Cargo genes
| Drug resistance gene | blaKPC-3 |
| Virulence gene | - |
| Metal resistance gene | merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |