Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120468 |
| Name | oriT_p11A14034org1_B_KPC  |
| Organism | Enterobacter hormaechei subsp. steigerwaltii strain 11A14CRGN034org1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OQ821069 (86423..86528 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_p11A14034org1_B_KPC
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12890 | GenBank | WP_004187323 |
| Name | traI_SLH58_RS00555_p11A14034org1_B_KPC |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 15139 | GenBank | WP_004187443 |
| Name | t4cp2_SLH58_RS00020_p11A14034org1_B_KPC |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79519.87 Da Isoelectric Point: 4.8309
>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 89324..108517
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SLH58_RS00530 (FEJLJNBH_00106) | 84408..84719 | + | 312 | WP_004187333 | hypothetical protein | - |
| SLH58_RS00535 (FEJLJNBH_00107) | 84891..85388 | + | 498 | WP_260466953 | hypothetical protein | - |
| SLH58_RS00540 (FEJLJNBH_00108) | 85458..85598 | + | 141 | WP_015062823 | hypothetical protein | - |
| SLH58_RS00545 (FEJLJNBH_00109) | 85890..86285 | - | 396 | WP_019725163 | hypothetical protein | - |
| SLH58_RS00550 (FEJLJNBH_00110) | 86284..86847 | + | 564 | WP_020277892 | plasmid mobilization protein MobA | - |
| SLH58_RS00555 (FEJLJNBH_00111) | 86834..88813 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| SLH58_RS00560 (FEJLJNBH_00112) | 88827..89327 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| SLH58_RS00565 (FEJLJNBH_00113) | 89324..90103 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| SLH58_RS00570 (FEJLJNBH_00114) | 90114..91277 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| SLH58_RS00575 (FEJLJNBH_00115) | 91267..91527 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| SLH58_RS00580 (FEJLJNBH_00116) | 91552..94854 | + | 3303 | WP_319382161 | LPD7 domain-containing protein | - |
| SLH58_RS00585 (FEJLJNBH_00117) | 94820..95332 | + | 513 | WP_011091071 | hypothetical protein | traL |
| SLH58_RS00590 (FEJLJNBH_00118) | 95333..95545 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| SLH58_RS00595 (FEJLJNBH_00119) | 95517..95927 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| SLH58_RS00600 (FEJLJNBH_00120) | 95926..96708 | + | 783 | WP_011091073 | DotI/IcmL family type IV secretion protein | traM |
| SLH58_RS00605 (FEJLJNBH_00121) | 96717..97868 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| SLH58_RS00610 (FEJLJNBH_00122) | 97880..99229 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| SLH58_RS00615 (FEJLJNBH_00123) | 99241..99945 | + | 705 | WP_031942491 | conjugal transfer protein TraP | traP |
| SLH58_RS00620 (FEJLJNBH_00124) | 99969..100499 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| SLH58_RS00625 (FEJLJNBH_00125) | 100516..100905 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| SLH58_RS00630 (FEJLJNBH_00126) | 100951..101445 | + | 495 | WP_011091080 | hypothetical protein | - |
| SLH58_RS00635 (FEJLJNBH_00127) | 101442..104492 | + | 3051 | WP_319382162 | conjugal transfer protein | traU |
| SLH58_RS00640 (FEJLJNBH_00128) | 104489..105697 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| SLH58_RS00645 (FEJLJNBH_00129) | 105694..106344 | + | 651 | WP_004187488 | hypothetical protein | - |
| SLH58_RS00650 | 106337..108517 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| SLH58_RS00655 (FEJLJNBH_00132) | 108520..109173 | + | 654 | WP_004206935 | hypothetical protein | - |
| SLH58_RS00660 (FEJLJNBH_00133) | 109247..109477 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 20897 | GenBank | NZ_OQ821069 |
| Plasmid name | p11A14034org1_B_KPC | Incompatibility group | IncL/M |
| Plasmid size | 109773 bp | Coordinate of oriT [Strand] | 86423..86528 [+] |
| Host baterium | Enterobacter hormaechei subsp. steigerwaltii strain 11A14CRGN034org1 |
Cargo genes
| Drug resistance gene | sul1, qnrB2, blaKPC-3, tet(A), ant(2'')-Ia, blaSHV-30 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |