Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120462
Name   oriT_pEFT30-2 in_silico
Organism   Enterococcus faecalis strain T30
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP113830 (5088..5123 [-], 36 nt)
oriT length   36 nt
IRs (inverted repeats)      1..8, 13..20  (GTAACATG..CATGTTAC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 36 nt

>oriT_pEFT30-2
GTAACATGCTATCATGTTACTATGCTCGCGAAAGGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12886 GenBank   WP_104670895
Name   Relaxase_OZE10_RS00395_pEFT30-2 insolico UniProt ID   _
Length   571 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 571 a.a.        Molecular weight: 66176.96 Da        Isoelectric Point: 6.1074

>WP_104670895.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFTIHTMEHLNQSKEYVQKASKTTVDKSSSGNTHYEQLFPYIMNADKTLSKQLVSGHGITDVTNA
ANEFLYTKERVAKQKGTYLMFDPETETMVFDKAAIEKGNGRGQAVLGHHLIQSFSPEDDLTPEEIHEIGR
QTVLEFTGGEHEFVIATHVDRKHIHNHIIINSTNLFTGKALPWKVVTFENGETKDYTKELFEKVSDKISA
KYGAKIIEKSPKNSHKKYTMWQTESIYKSKIKSRLDFLLDHSSSIEDFKCKAEALELHVDFSGKWATYRL
LDEPQIKNTRSRSLNKKNPEKYNLKQIEERLKENTASFSIEEVVDRYEEKINVVKNDFDYQLIVEPWQIS
HKTPKGYYLNVDFGIENHGQIFLGAYKVDELEDGNYSLFVKKNDFFYFMNEKNGQRNRYMTGYTLAKQLS
LYNGTVPLKKEPVMSTIDEIVDAINFLAEHDVIEGNQMERLEKQLVEVFKTAEESLETLDEKILQLNQVG
KLLLANELENNKENLPILKQLEEIEELTYEDVKNELTSLKLSRKLLQEKMETTVEKINELHGVQTVVERK
TKEENLGKIEK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(37-310)


  Protein structure



No available structure.




T4CP

ID   15135 GenBank   WP_104670887
Name   t4cp2_OZE10_RS00435_pEFT30-2 insolico UniProt ID   _
Length   603 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 603 a.a.        Molecular weight: 69893.94 Da        Isoelectric Point: 6.5211

>WP_104670887.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MQVYKKEFKPYLMLAICLFFVGYALGNFFWLLPGYDYFSKMNYLFTKDLLSYANENFFLFFFTPSFLAFG
TGFLGFLIGLLAYVRDNDRGIYRNGEEYGSARFATDEEMAKFSDKDPKNNMIISKKVQIGLFNFRLPIKI
QKNKNVMVVGDSGSAKTLAYILTNILQLNASFVVTDPDGGIVHKVGKLLKKFGYKIKILDLNQLVNSDTF
NVFQYIRTELDIDRVLEAITEGTKKTEQQGEDFWIQAEALLIRSFIAYLWFDGQDNDYMPNLSMIADMLR
HVERKDKKVPSPVEQWFEEQNQLRPNNYAYKQWTLYNDSYKAETRASVLAIASARYSVFDHEQVADMIRK
DTMEIDKWNEEKTAVFIAIPETNKSYNFIAAIFMATVMETLRHKSDQVRLGKLQLPKEKKLLHVRFLIDE
FANIGRIPNFEEALATFRKREMSFSIILQSLAQLQKMYKYGWEGIVNNCSTLLFLGGDEEKTTKYLSHRA
DKQTISLRKYSKSHGRNGGGSESRDRTGRDLLTPGEIGHLDGDECLVYISKEYVYKDKKYYAFDHPLGSM
MGDEPGDENWYTYKRYLTEEEEILDKVRPENIIDHGVIGDEVA

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(117-555)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 13803..29415

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OZE10_RS00420 (OZE10_00420) 10543..10743 - 201 WP_104670890 hypothetical protein -
OZE10_RS00425 (OZE10_00425) 10833..11306 - 474 WP_104670889 single-stranded DNA-binding protein -
OZE10_RS00430 (OZE10_00430) 11344..11907 - 564 WP_104670888 hypothetical protein -
OZE10_RS00435 (OZE10_00435) 11992..13803 - 1812 WP_104670887 VirD4-like conjugal transfer protein, CD1115 family -
OZE10_RS00440 (OZE10_00440) 13803..14288 - 486 WP_161975848 DUF3801 domain-containing protein gbs1365
OZE10_RS00445 (OZE10_00445) 14330..14809 - 480 WP_104670885 hypothetical protein -
OZE10_RS00450 (OZE10_00450) 14832..15062 - 231 WP_010711013 hypothetical protein -
OZE10_RS00455 (OZE10_00455) 15055..15669 - 615 WP_104670884 hypothetical protein prgL
OZE10_RS00460 (OZE10_00460) 15709..18324 - 2616 WP_104670883 phage tail tip lysozyme prgK
OZE10_RS00465 (OZE10_00465) 18336..20723 - 2388 WP_161975846 VirB4-like conjugal transfer ATPase, CD1110 family virb4
OZE10_RS00470 (OZE10_00470) 20677..21030 - 354 WP_104670882 PrgI family mobile element protein prgIc
OZE10_RS00475 (OZE10_00475) 21032..21823 - 792 WP_104670881 type III secretion system protein PrgH prgHb
OZE10_RS00480 (OZE10_00480) 21836..22063 - 228 WP_104670880 type III secretion system protein PrgF -
OZE10_RS00485 (OZE10_00485) 22088..22522 - 435 WP_104670879 type III secretion system protein PrgE -
OZE10_RS00490 (OZE10_00490) 22541..23437 - 897 WP_104670878 type III secretion system protein PrgD -
OZE10_RS00495 (OZE10_00495) 23755..24609 - 855 WP_104670877 pheromone response LPXTG-anchored adhesin PrgC prgC
OZE10_RS00500 (OZE10_00500) 24637..24993 - 357 WP_010711024 pheromone response system RNA-binding regulator PrgU -
OZE10_RS00505 (OZE10_00505) 25057..25197 - 141 WP_104670876 LPXTG cell wall anchor domain-containing protein prgC
OZE10_RS00510 (OZE10_00510) 25537..29415 - 3879 WP_104670964 LPXTG-anchored aggregation substance prgB
OZE10_RS00515 (OZE10_00515) 29731..30054 - 324 WP_002383600 DUF5406 family protein -
OZE10_RS00525 (OZE10_00525) 30513..30791 - 279 WP_104670904 hypothetical protein -
OZE10_RS00530 (OZE10_00530) 30811..33483 - 2673 WP_104670903 surface exclusion protein SEA1/PrgA -
OZE10_RS00535 (OZE10_00535) 33494..33799 - 306 WP_002367666 hypothetical protein -
OZE10_RS15290 34101..34322 - 222 WP_002394860 hypothetical protein -

Host bacterium

ID   20891 GenBank   NZ_CP113830
Plasmid name   pEFT30-2 Incompatibility group   -
Plasmid size   49275 bp Coordinate of oriT [Strand]   5088..5123 [-]
Host baterium   Enterococcus faecalis strain T30

Cargo genes

Drug resistance gene   -
Virulence gene   asa1, prgB/asc10
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -