Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120458
Name   oriT_pF321-3 in_silico
Organism   Citrobacter portucalensis strain F321
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP137479 (8730..8866 [-], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      28..33, 37..42  (ACATGC..GCATGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_pF321-3
CGAATGGGCAGGCGGGGATTGGCAGGAACATGCGTAGCATGTGTATGACAATCCGCATCCTGTATTGCTCAACCACTATACTATCATATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCATAAAAGGCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12882 GenBank   WP_284302396
Name   traI_R4T15_RS24650_pF321-3 insolico UniProt ID   _
Length   890 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 890 a.a.        Molecular weight: 101944.45 Da        Isoelectric Point: 9.7193

>WP_284302396.1 TraI/MobA(P) family conjugative relaxase [Citrobacter portucalensis]
MIVKKVPNPKKSASKAQRIGQLTSYVRSPESESPQEKCLYAGARGFMMDDPKSQTAEMIALSQEAVRSKD
TINHYVLSWREGEQPSPEQVEEAVSIFMDELGWKDHQAIYGLHSDTDNIHLHIVINRVHPETLKIVEKNR
GFDIELAHKAIARIEHAQGWQREQNGRYQVLENGELGRAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAI
EDGAAIIKQAQTWEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQR
QQVAPREPEPIKPDVPGWKDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDELMRGNWKGKG
EVLNAMRSVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
DRSEPPTPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGS
FTVMGNDEYKAMCGKLAAEHGFKITNPELQESIQQERQRIQQERVQAMKSEQLKQFERYAEAVGAERYRV
TSIKMREDGSKQTFILDKKDSITRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKL
ERLIRDGYQPAAVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
KPKHQREDGSYPEVRLLKAERRECIKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
YQRHYRDVLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSL

  Protein domains


Predicted by InterproScan.

RepB DNA-primase from phage plasmid (RepB_primase)

(548-807)

Relaxase/Mobilisation nuclease domain (Relaxase)

(45-274)

Large polyvalent protein-associated domain 7 (LPD7)

(439-527)


  Protein structure



No available structure.




Host bacterium

ID   20887 GenBank   NZ_CP137479
Plasmid name   pF321-3 Incompatibility group   IncQ2
Plasmid size   12945 bp Coordinate of oriT [Strand]   8730..8866 [-]
Host baterium   Citrobacter portucalensis strain F321

Cargo genes

Drug resistance gene   mph(E), msr(E)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -