Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120447 |
| Name | oriT_MGH103|unnamed2 |
| Organism | Citrobacter sp. MGH103 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP073045 (108696..108819 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 92..99, 113..120 (ATAATGTA..TACATTAT) 90..95, 107..112 (AAATAA..TTATTT) 39..46, 49..56 (GCAAAAAC..GTTTTTGC) 3..10, 15..22 (TTGGTGGT..ACCACCAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
GGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTGCTACTTGAATCATTAACTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATAAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12876 | GenBank | WP_001617867 |
| Name | traI_SK33_RS25890_MGH103|unnamed2 |
UniProt ID | A0A2Z5WSA2 |
| Length | 1756 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 191679.38 Da Isoelectric Point: 5.8498
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFPPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTEAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDEPRTAEKLARHPSFTVVSDQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGTSETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGRGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTELAVRDIAGQERDRTASSERETALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7256 | GenBank | WP_001254388 |
| Name | WP_001254388_MGH103|unnamed2 |
UniProt ID | W9A0Z2 |
| Length | 75 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 75 a.a. Molecular weight: 9072.20 Da Isoelectric Point: 10.2071
MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVIEENES
TFKEL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | W9A0Z2 |
| ID | 7257 | GenBank | WP_001151566 |
| Name | WP_001151566_MGH103|unnamed2 |
UniProt ID | A0A142CMX1 |
| Length | 127 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14476.46 Da Isoelectric Point: 4.8498
MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLELGLRVYETQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPENDEG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142CMX1 |
T4CP
| ID | 15124 | GenBank | WP_021547900 |
| Name | traD_SK33_RS25895_MGH103|unnamed2 |
UniProt ID | _ |
| Length | 735 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 735 a.a. Molecular weight: 83494.17 Da Isoelectric Point: 5.1657
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLILWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 15125 | GenBank | WP_001064258 |
| Name | traC_SK33_RS26000_MGH103|unnamed2 |
UniProt ID | _ |
| Length | 875 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 875 a.a. Molecular weight: 99195.72 Da Isoelectric Point: 5.8689
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLKSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQYT
ANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNYKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFQPLQRDMIGKFGAARDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 80557..109391
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SK33_RS25895 | 80557..82764 | - | 2208 | WP_021547900 | type IV conjugative transfer system coupling protein TraD | virb4 |
| SK33_RS25900 | 82761..83540 | - | 780 | WP_000199914 | hypothetical protein | - |
| SK33_RS25905 | 83743..84474 | - | 732 | WP_000850429 | conjugal transfer complement resistance protein TraT | - |
| SK33_RS25910 | 84506..85003 | - | 498 | WP_000605862 | entry exclusion protein | - |
| SK33_RS25915 | 85022..87841 | - | 2820 | WP_001007062 | conjugal transfer mating-pair stabilization protein TraG | traG |
| SK33_RS25920 | 87838..89211 | - | 1374 | WP_000944328 | conjugal transfer pilus assembly protein TraH | traH |
| SK33_RS25925 | 89198..89590 | - | 393 | WP_000659962 | F-type conjugal transfer protein TrbF | - |
| SK33_RS25930 | 89571..89918 | - | 348 | WP_071571857 | P-type conjugative transfer protein TrbJ | - |
| SK33_RS25935 | 89848..90393 | - | 546 | WP_000059829 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| SK33_RS25940 | 90380..90664 | - | 285 | WP_001617873 | type-F conjugative transfer system pilin chaperone TraQ | - |
| SK33_RS25945 | 90791..91111 | - | 321 | WP_001348757 | conjugal transfer protein TrbA | - |
| SK33_RS25955 | 92011..92991 | - | 981 | WP_000019450 | IS5-like element ISKpn26 family transposase | - |
| SK33_RS25960 | 93121..93864 | - | 744 | WP_001030401 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| SK33_RS25965 | 93857..94114 | - | 258 | WP_000864347 | conjugal transfer protein TrbE | - |
| SK33_RS25970 | 94141..95991 | - | 1851 | WP_000821866 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| SK33_RS25975 | 95988..96626 | - | 639 | WP_001080259 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| SK33_RS25980 | 96635..96940 | - | 306 | WP_000224413 | hypothetical protein | - |
| SK33_RS25985 | 96970..97962 | - | 993 | WP_000830190 | conjugal transfer pilus assembly protein TraU | traU |
| SK33_RS25990 | 97959..98591 | - | 633 | WP_001203727 | type-F conjugative transfer system protein TraW | traW |
| SK33_RS25995 | 98588..98974 | - | 387 | WP_000214084 | type-F conjugative transfer system protein TrbI | - |
| SK33_RS26000 | 98971..101598 | - | 2628 | WP_001064258 | type IV secretion system protein TraC | virb4 |
| SK33_RS26005 | 101758..101979 | - | 222 | WP_001278694 | conjugal transfer protein TraR | - |
| SK33_RS26010 | 102114..102629 | - | 516 | WP_000809886 | type IV conjugative transfer system lipoprotein TraV | traV |
| SK33_RS26015 | 102629..102940 | - | 312 | WP_001057272 | conjugal transfer protein TrbD | - |
| SK33_RS26020 | 102927..103493 | - | 567 | WP_001617877 | conjugal transfer pilus-stabilizing protein TraP | - |
| SK33_RS26025 | 103483..104934 | - | 1452 | WP_000146687 | F-type conjugal transfer pilus assembly protein TraB | traB |
| SK33_RS26030 | 104934..105662 | - | 729 | WP_001230808 | type-F conjugative transfer system secretin TraK | traK |
| SK33_RS26035 | 105649..106215 | - | 567 | WP_000399791 | type IV conjugative transfer system protein TraE | traE |
| SK33_RS26040 | 106237..106548 | - | 312 | WP_000012107 | type IV conjugative transfer system protein TraL | traL |
| SK33_RS26045 | 106553..106915 | - | 363 | WP_000338606 | type IV conjugative transfer system pilin TraA | - |
| SK33_RS26050 | 106949..107176 | - | 228 | WP_001254388 | conjugal transfer relaxosome protein TraY | - |
| SK33_RS26055 | 107264..107941 | - | 678 | WP_001348626 | PAS domain-containing protein | - |
| SK33_RS26060 | 108075..108458 | - | 384 | WP_001151566 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| SK33_RS26065 | 108801..109391 | + | 591 | WP_001376243 | transglycosylase SLT domain-containing protein | virB1 |
| SK33_RS26070 | 109688..110509 | - | 822 | WP_001234469 | DUF932 domain-containing protein | - |
| SK33_RS26075 | 110628..110915 | - | 288 | WP_000107535 | hypothetical protein | - |
| SK33_RS26080 | 110940..111146 | - | 207 | WP_000275859 | hypothetical protein | - |
| SK33_RS26580 | 111216..111389 | + | 174 | Protein_138 | hypothetical protein | - |
| SK33_RS26585 | 111387..111617 | - | 231 | WP_001426396 | hypothetical protein | - |
| SK33_RS26085 | 111837..111962 | - | 126 | WP_001372321 | type I toxin-antitoxin system Hok family toxin | - |
| SK33_RS26590 | 111904..112053 | - | 150 | Protein_141 | plasmid maintenance protein Mok | - |
| SK33_RS26090 | 112275..112994 | - | 720 | WP_001276232 | plasmid SOS inhibition protein A | - |
| SK33_RS26095 | 112991..113425 | - | 435 | WP_000845940 | conjugation system SOS inhibitor PsiB | - |
Host bacterium
| ID | 20876 | GenBank | NZ_CP073045 |
| Plasmid name | MGH103|unnamed2 | Incompatibility group | IncFIB |
| Plasmid size | 145482 bp | Coordinate of oriT [Strand] | 108696..108819 [-] |
| Host baterium | Citrobacter sp. MGH103 |
Cargo genes
| Drug resistance gene | tet(B), dfrA14, mph(A), sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |