Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   120447
Name   oriT_MGH103|unnamed2 in_silico
Organism   Citrobacter sp. MGH103
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP073045 (108696..108819 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      92..99, 113..120  (ATAATGTA..TACATTAT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_MGH103|unnamed2
GGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTGCTACTTGAATCATTAACTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATAAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   12876 GenBank   WP_001617867
Name   traI_SK33_RS25890_MGH103|unnamed2 insolico UniProt ID   A0A2Z5WSA2
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191679.38 Da        Isoelectric Point: 5.8498

>WP_001617867.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFPPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTEAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDEPRTAEKLARHPSFTVVSDQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGTSETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGRGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTELAVRDIAGQERDRTASSERETALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(10-283)

(968-1156)

(633-712)

(1434-1557)

(575-626)


  Protein structure



No available structure.




Auxiliary protein


ID   7256 GenBank   WP_001254388
Name   WP_001254388_MGH103|unnamed2 insolico UniProt ID   W9A0Z2
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9072.20 Da        Isoelectric Point: 10.2071

>WP_001254388.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Enterobacterales]
MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVIEENES
TFKEL

  Protein domains


Predicted by InterproScan.

(14-61)


  Protein structure


Source ID Structure
AlphaFold DB W9A0Z2

ID   7257 GenBank   WP_001151566
Name   WP_001151566_MGH103|unnamed2 insolico UniProt ID   A0A142CMX1
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14476.46 Da        Isoelectric Point: 4.8498

>WP_001151566.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacterales]
MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLELGLRVYETQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPENDEG

  Protein domains


Predicted by InterproScan.

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A142CMX1


T4CP


ID   15124 GenBank   WP_021547900
Name   traD_SK33_RS25895_MGH103|unnamed2 insolico UniProt ID   _
Length   735 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 735 a.a.        Molecular weight: 83494.17 Da        Isoelectric Point: 5.1657

>WP_021547900.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLILWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(32-128)

(173-560)

  Protein structure



No available structure.



ID   15125 GenBank   WP_001064258
Name   traC_SK33_RS26000_MGH103|unnamed2 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99195.72 Da        Isoelectric Point: 5.8689

>WP_001064258.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLKSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQYT
ANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNYKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFQPLQRDMIGKFGAARDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(289-446)

(38-276)

(467-763)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 80557..109391

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SK33_RS25895 80557..82764 - 2208 WP_021547900 type IV conjugative transfer system coupling protein TraD virb4
SK33_RS25900 82761..83540 - 780 WP_000199914 hypothetical protein -
SK33_RS25905 83743..84474 - 732 WP_000850429 conjugal transfer complement resistance protein TraT -
SK33_RS25910 84506..85003 - 498 WP_000605862 entry exclusion protein -
SK33_RS25915 85022..87841 - 2820 WP_001007062 conjugal transfer mating-pair stabilization protein TraG traG
SK33_RS25920 87838..89211 - 1374 WP_000944328 conjugal transfer pilus assembly protein TraH traH
SK33_RS25925 89198..89590 - 393 WP_000659962 F-type conjugal transfer protein TrbF -
SK33_RS25930 89571..89918 - 348 WP_071571857 P-type conjugative transfer protein TrbJ -
SK33_RS25935 89848..90393 - 546 WP_000059829 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
SK33_RS25940 90380..90664 - 285 WP_001617873 type-F conjugative transfer system pilin chaperone TraQ -
SK33_RS25945 90791..91111 - 321 WP_001348757 conjugal transfer protein TrbA -
SK33_RS25955 92011..92991 - 981 WP_000019450 IS5-like element ISKpn26 family transposase -
SK33_RS25960 93121..93864 - 744 WP_001030401 type-F conjugative transfer system pilin assembly protein TraF traF
SK33_RS25965 93857..94114 - 258 WP_000864347 conjugal transfer protein TrbE -
SK33_RS25970 94141..95991 - 1851 WP_000821866 type-F conjugative transfer system mating-pair stabilization protein TraN traN
SK33_RS25975 95988..96626 - 639 WP_001080259 type-F conjugative transfer system pilin assembly protein TrbC trbC
SK33_RS25980 96635..96940 - 306 WP_000224413 hypothetical protein -
SK33_RS25985 96970..97962 - 993 WP_000830190 conjugal transfer pilus assembly protein TraU traU
SK33_RS25990 97959..98591 - 633 WP_001203727 type-F conjugative transfer system protein TraW traW
SK33_RS25995 98588..98974 - 387 WP_000214084 type-F conjugative transfer system protein TrbI -
SK33_RS26000 98971..101598 - 2628 WP_001064258 type IV secretion system protein TraC virb4
SK33_RS26005 101758..101979 - 222 WP_001278694 conjugal transfer protein TraR -
SK33_RS26010 102114..102629 - 516 WP_000809886 type IV conjugative transfer system lipoprotein TraV traV
SK33_RS26015 102629..102940 - 312 WP_001057272 conjugal transfer protein TrbD -
SK33_RS26020 102927..103493 - 567 WP_001617877 conjugal transfer pilus-stabilizing protein TraP -
SK33_RS26025 103483..104934 - 1452 WP_000146687 F-type conjugal transfer pilus assembly protein TraB traB
SK33_RS26030 104934..105662 - 729 WP_001230808 type-F conjugative transfer system secretin TraK traK
SK33_RS26035 105649..106215 - 567 WP_000399791 type IV conjugative transfer system protein TraE traE
SK33_RS26040 106237..106548 - 312 WP_000012107 type IV conjugative transfer system protein TraL traL
SK33_RS26045 106553..106915 - 363 WP_000338606 type IV conjugative transfer system pilin TraA -
SK33_RS26050 106949..107176 - 228 WP_001254388 conjugal transfer relaxosome protein TraY -
SK33_RS26055 107264..107941 - 678 WP_001348626 PAS domain-containing protein -
SK33_RS26060 108075..108458 - 384 WP_001151566 conjugal transfer relaxosome DNA-binding protein TraM -
SK33_RS26065 108801..109391 + 591 WP_001376243 transglycosylase SLT domain-containing protein virB1
SK33_RS26070 109688..110509 - 822 WP_001234469 DUF932 domain-containing protein -
SK33_RS26075 110628..110915 - 288 WP_000107535 hypothetical protein -
SK33_RS26080 110940..111146 - 207 WP_000275859 hypothetical protein -
SK33_RS26580 111216..111389 + 174 Protein_138 hypothetical protein -
SK33_RS26585 111387..111617 - 231 WP_001426396 hypothetical protein -
SK33_RS26085 111837..111962 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
SK33_RS26590 111904..112053 - 150 Protein_141 plasmid maintenance protein Mok -
SK33_RS26090 112275..112994 - 720 WP_001276232 plasmid SOS inhibition protein A -
SK33_RS26095 112991..113425 - 435 WP_000845940 conjugation system SOS inhibitor PsiB -


Host bacterium


ID   20876 GenBank   NZ_CP073045
Plasmid name   MGH103|unnamed2 Incompatibility group   IncFIB
Plasmid size   145482 bp Coordinate of oriT [Strand]   108696..108819 [-]
Host baterium   Citrobacter sp. MGH103

Cargo genes


Drug resistance gene   tet(B), dfrA14, mph(A), sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -