Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120429
Name   oriT_p41A20009_B_KPC_KPC in_silico
Organism   Citrobacter freundii strain 41A20CPO009
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OQ821210 (12933..13033 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_p41A20009_B_KPC_KPC
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12865 GenBank   WP_012561166
Name   mobF_SLH58_RS00045_p41A20009_B_KPC_KPC insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   7254 GenBank   WP_001749975
Name   WP_001749975_p41A20009_B_KPC_KPC insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   15113 GenBank   WP_000342688
Name   t4cp2_SLH58_RS00050_p41A20009_B_KPC_KPC insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 47139..57464

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SLH58_RS00250 (CMEMAFDF_00051) 42984..44417 - 1434 WP_001288432 DNA cytosine methyltransferase -
SLH58_RS00255 (CMEMAFDF_00052) 44516..44788 + 273 Protein_50 IS1 family transposase -
SLH58_RS00260 (CMEMAFDF_00053) 44799..45005 - 207 WP_001749967 hypothetical protein -
SLH58_RS00265 (CMEMAFDF_00054) 45010..45657 - 648 WP_015344958 restriction endonuclease -
SLH58_RS00270 (CMEMAFDF_00055) 45707..45940 - 234 WP_001191790 hypothetical protein -
SLH58_RS00275 (CMEMAFDF_00056) 46054..46368 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
SLH58_RS00280 (CMEMAFDF_00057) 46365..46709 - 345 WP_001749964 hypothetical protein -
SLH58_RS00285 (CMEMAFDF_00058) 46725..47096 - 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
SLH58_RS00290 (CMEMAFDF_00059) 47139..47873 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
SLH58_RS00295 (CMEMAFDF_00060) 47882..48163 + 282 WP_000440698 transcriptional repressor KorA -
SLH58_RS00300 (CMEMAFDF_00061) 48173..48466 + 294 WP_001749962 hypothetical protein virB2
SLH58_RS00305 (CMEMAFDF_00062) 48516..48833 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
SLH58_RS00310 (CMEMAFDF_00063) 48833..51433 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
SLH58_RS00315 (CMEMAFDF_00064) 51451..52164 + 714 WP_001749960 type IV secretion system protein virB5
SLH58_RS00320 (CMEMAFDF_00065) 52172..52399 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
SLH58_RS00325 (CMEMAFDF_00066) 52415..53455 + 1041 WP_001749958 type IV secretion system protein virB6
SLH58_RS00330 53526..53684 + 159 WP_012561180 hypothetical protein -
SLH58_RS00335 (CMEMAFDF_00067) 53674..54372 + 699 WP_000646594 virB8 family protein virB8
SLH58_RS00340 (CMEMAFDF_00068) 54383..55267 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
SLH58_RS00345 (CMEMAFDF_00069) 55267..56427 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
SLH58_RS00350 (CMEMAFDF_00070) 56469..57464 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
SLH58_RS00355 (CMEMAFDF_00071) 57464..57997 + 534 WP_000792636 phospholipase D family protein -
SLH58_RS00360 58171..58299 - 129 WP_002210551 DUF6710 family protein -
SLH58_RS00365 (CMEMAFDF_00072) 58394..61222 - 2829 Protein_72 Tn3 family transposase -

Host bacterium

ID   20858 GenBank   NZ_OQ821210
Plasmid name   p41A20009_B_KPC_KPC Incompatibility group   IncN
Plasmid size   84804 bp Coordinate of oriT [Strand]   12933..13033 [+]
Host baterium   Citrobacter freundii strain 41A20CPO009

Cargo genes

Drug resistance gene   blaKPC-3, aac(6')-Ib-cr, ARR-3, dfrA27, aadA16, qacE, sul1, qnrB6
Virulence gene   -
Metal resistance gene   arsR, arsD, arsA, arsB, arsC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -