Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   120417
Name   oriT_p31B19014_B_KPC in_silico
Organism   Enterobacter hormaechei subsp. oharae strain 31B19CPO014
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OQ821192 (62182..62287 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_p31B19014_B_KPC
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   12856 GenBank   WP_004187323
Name   traI_SLH58_RS00445_p31B19014_B_KPC insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP


ID   15102 GenBank   WP_038874906
Name   t4cp2_SLH58_RS00170_p31B19014_B_KPC insolico UniProt ID   _
Length   701 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 701 a.a.        Molecular weight: 80068.49 Da        Isoelectric Point: 4.8705

>WP_038874906.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAESNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIGVVSEFG
K

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 65083..84321

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SLH58_RS00425 (LFBPNDPG_00079) 60169..60480 + 312 WP_004187333 hypothetical protein -
SLH58_RS00430 (LFBPNDPG_00080) 60612..61148 + 537 WP_011091066 hypothetical protein -
SLH58_RS00435 (LFBPNDPG_00082) 61649..62044 - 396 WP_020805633 hypothetical protein -
SLH58_RS00440 62043..62606 + 564 WP_319382137 plasmid mobilization protein MobA -
SLH58_RS00445 (LFBPNDPG_00084) 62593..64572 + 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
SLH58_RS00450 (LFBPNDPG_00085) 64586..65086 + 501 WP_004187320 DotD/TraH family lipoprotein -
SLH58_RS00455 (LFBPNDPG_00086) 65083..65862 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
SLH58_RS00460 (LFBPNDPG_00087) 65873..67036 + 1164 WP_020805653 plasmid transfer ATPase TraJ virB11
SLH58_RS00465 (LFBPNDPG_00088) 67026..67286 + 261 WP_004187310 IcmT/TraK family protein traK
SLH58_RS00470 (LFBPNDPG_00089) 67311..70658 + 3348 WP_319382220 LPD7 domain-containing protein -
SLH58_RS00475 (LFBPNDPG_00090) 70582..71136 + 555 WP_011154467 hypothetical protein traL
SLH58_RS00480 (LFBPNDPG_00091) 71137..71334 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
SLH58_RS00485 (LFBPNDPG_00092) 71321..71731 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
SLH58_RS00490 (LFBPNDPG_00093) 71730..72512 + 783 WP_004206929 DotI/IcmL family type IV secretion protein traM
SLH58_RS00495 (LFBPNDPG_00094) 72548..73672 + 1125 WP_319382139 DotH/IcmK family type IV secretion protein traN
SLH58_RS00500 (LFBPNDPG_00095) 73684..75033 + 1350 WP_020805691 conjugal transfer protein TraO traO
SLH58_RS00505 (LFBPNDPG_00096) 75045..75749 + 705 WP_020805696 conjugal transfer protein TraP traP
SLH58_RS00510 (LFBPNDPG_00097) 75773..76303 + 531 WP_011091077 conjugal transfer protein TraQ traQ
SLH58_RS00515 (LFBPNDPG_00098) 76320..76709 + 390 WP_020805690 DUF6750 family protein traR
SLH58_RS00520 (LFBPNDPG_00099) 76755..77249 + 495 WP_223294957 hypothetical protein -
SLH58_RS00525 (LFBPNDPG_00100) 77246..80296 + 3051 WP_020805687 ATPase AAA traU
SLH58_RS00530 (LFBPNDPG_00101) 80326..81501 + 1176 WP_167577398 conjugal transfer protein TraW traW
SLH58_RS00535 (LFBPNDPG_00102) 81498..82148 + 651 WP_032426814 hypothetical protein -
SLH58_RS00540 (LFBPNDPG_00103) 82141..84321 + 2181 WP_032426815 DotA/TraY family protein traY
SLH58_RS00545 (LFBPNDPG_00104) 84324..84977 + 654 WP_020805693 hypothetical protein -
SLH58_RS00550 (LFBPNDPG_00105) 85039..85281 + 243 WP_023893611 IncL/M type plasmid replication protein RepC -


Host bacterium


ID   20846 GenBank   NZ_OQ821192
Plasmid name   p31B19014_B_KPC Incompatibility group   IncL/M
Plasmid size   85577 bp Coordinate of oriT [Strand]   62182..62287 [+]
Host baterium   Enterobacter hormaechei subsp. oharae strain 31B19CPO014

Cargo genes


Drug resistance gene   blaKPC-3, blaTEM-1B, ant(2'')-Ia, aadA5, blaOXA-2, qacE, sul1
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -