Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120415 |
| Name | oriT_p31B19029_B_KPC  |
| Organism | Enterobacter hormaechei subsp. hormaechei strain 31B19CPO029 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OQ821193 (62182..62287 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_p31B19029_B_KPC
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12854 | GenBank | WP_004187323 |
| Name | traI_SLH58_RS00445_p31B19029_B_KPC |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 15100 | GenBank | WP_038874906 |
| Name | t4cp2_SLH58_RS00170_p31B19029_B_KPC |
UniProt ID | _ |
| Length | 701 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 701 a.a. Molecular weight: 80068.49 Da Isoelectric Point: 4.8705
>WP_038874906.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAESNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIGVVSEFG
K
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAESNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIGVVSEFG
K
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 65083..84411
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SLH58_RS00425 (BKDMAFAF_00079) | 60169..60480 | + | 312 | WP_004187333 | hypothetical protein | - |
| SLH58_RS00430 (BKDMAFAF_00080) | 60612..61148 | + | 537 | WP_011091066 | hypothetical protein | - |
| SLH58_RS00435 (BKDMAFAF_00082) | 61649..62044 | - | 396 | WP_020805633 | hypothetical protein | - |
| SLH58_RS00440 | 62043..62606 | + | 564 | WP_319382137 | plasmid mobilization protein MobA | - |
| SLH58_RS00445 (BKDMAFAF_00084) | 62593..64572 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| SLH58_RS00450 (BKDMAFAF_00085) | 64586..65086 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| SLH58_RS00455 (BKDMAFAF_00086) | 65083..65862 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| SLH58_RS00460 (BKDMAFAF_00087) | 65873..67036 | + | 1164 | WP_020805653 | plasmid transfer ATPase TraJ | virB11 |
| SLH58_RS00465 (BKDMAFAF_00088) | 67026..67286 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| SLH58_RS00470 (BKDMAFAF_00089) | 67311..70748 | + | 3438 | WP_319382138 | LPD7 domain-containing protein | - |
| SLH58_RS00475 (BKDMAFAF_00090) | 70672..71226 | + | 555 | WP_011154467 | hypothetical protein | traL |
| SLH58_RS00480 (BKDMAFAF_00091) | 71227..71424 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| SLH58_RS00485 (BKDMAFAF_00092) | 71411..71821 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| SLH58_RS00490 (BKDMAFAF_00093) | 71820..72602 | + | 783 | WP_004206929 | DotI/IcmL family type IV secretion protein | traM |
| SLH58_RS00495 (BKDMAFAF_00094) | 72638..73762 | + | 1125 | WP_319382139 | DotH/IcmK family type IV secretion protein | traN |
| SLH58_RS00500 (BKDMAFAF_00095) | 73774..75123 | + | 1350 | WP_020805691 | conjugal transfer protein TraO | traO |
| SLH58_RS00505 (BKDMAFAF_00096) | 75135..75839 | + | 705 | WP_020805696 | conjugal transfer protein TraP | traP |
| SLH58_RS00510 (BKDMAFAF_00097) | 75863..76393 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| SLH58_RS00515 (BKDMAFAF_00098) | 76410..76799 | + | 390 | WP_020805690 | DUF6750 family protein | traR |
| SLH58_RS00520 (BKDMAFAF_00099) | 76845..77339 | + | 495 | WP_223294957 | hypothetical protein | - |
| SLH58_RS00525 (BKDMAFAF_00100) | 77336..80386 | + | 3051 | WP_020805687 | ATPase AAA | traU |
| SLH58_RS00530 (BKDMAFAF_00101) | 80416..81591 | + | 1176 | WP_167577398 | conjugal transfer protein TraW | traW |
| SLH58_RS00535 (BKDMAFAF_00102) | 81588..82238 | + | 651 | WP_032426814 | hypothetical protein | - |
| SLH58_RS00540 (BKDMAFAF_00103) | 82231..84411 | + | 2181 | WP_032426815 | DotA/TraY family protein | traY |
| SLH58_RS00545 (BKDMAFAF_00104) | 84414..85067 | + | 654 | WP_020805693 | hypothetical protein | - |
| SLH58_RS00550 (BKDMAFAF_00105) | 85129..85371 | + | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 20844 | GenBank | NZ_OQ821193 |
| Plasmid name | p31B19029_B_KPC | Incompatibility group | IncL/M |
| Plasmid size | 85667 bp | Coordinate of oriT [Strand] | 62182..62287 [+] |
| Host baterium | Enterobacter hormaechei subsp. hormaechei strain 31B19CPO029 |
Cargo genes
| Drug resistance gene | blaKPC-3, blaTEM-1B, ant(2'')-Ia, aadA5, blaOXA-2, qacE, sul1 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |