Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120409
Name   oriT_p24A18024_B_KPC in_silico
Organism   Enterobacter hormaechei subsp. steigerwaltii strain 24A18CPO024
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OQ821143 (48846..48951 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_p24A18024_B_KPC
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12848 GenBank   WP_004187323
Name   traI_SLH58_RS00335_p24A18024_B_KPC insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP

ID   15094 GenBank   WP_038874906
Name   t4cp2_SLH58_RS00060_p24A18024_B_KPC insolico UniProt ID   _
Length   701 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 701 a.a.        Molecular weight: 80068.49 Da        Isoelectric Point: 4.8705

>WP_038874906.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAESNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIGVVSEFG
K

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 51747..77119

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SLH58_RS00315 (JBLGJADJ_00061) 46833..47144 + 312 WP_004187333 hypothetical protein -
SLH58_RS00320 (JBLGJADJ_00062) 47276..47812 + 537 WP_011091066 hypothetical protein -
SLH58_RS00325 (JBLGJADJ_00064) 48313..48708 - 396 WP_020805633 hypothetical protein -
SLH58_RS00330 48707..49270 + 564 WP_319382137 plasmid mobilization protein MobA -
SLH58_RS00335 (JBLGJADJ_00066) 49257..51236 + 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
SLH58_RS00340 (JBLGJADJ_00067) 51250..51750 + 501 WP_004187320 DotD/TraH family lipoprotein -
SLH58_RS00345 (JBLGJADJ_00068) 51747..52526 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
SLH58_RS00350 (JBLGJADJ_00069) 52537..53700 + 1164 WP_020805653 plasmid transfer ATPase TraJ virB11
SLH58_RS00355 (JBLGJADJ_00070) 53690..53950 + 261 WP_004187310 IcmT/TraK family protein traK
SLH58_RS00360 (JBLGJADJ_00071) 53975..57232 + 3258 WP_319382209 LPD7 domain-containing protein -
SLH58_RS00365 (JBLGJADJ_00072) 57156..57710 + 555 WP_011154467 hypothetical protein traL
SLH58_RS00370 (JBLGJADJ_00073) 57711..57908 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
SLH58_RS00375 (JBLGJADJ_00074) 57895..58305 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
SLH58_RS00380 (JBLGJADJ_00075) 58304..59086 + 783 WP_004206929 DotI/IcmL family type IV secretion protein traM
SLH58_RS00385 (JBLGJADJ_00076) 59122..60246 + 1125 WP_319382139 DotH/IcmK family type IV secretion protein traN
SLH58_RS00390 (JBLGJADJ_00077) 60258..61607 + 1350 WP_020805691 conjugal transfer protein TraO traO
SLH58_RS00395 (JBLGJADJ_00078) 61619..62323 + 705 WP_020805696 conjugal transfer protein TraP traP
SLH58_RS00400 (JBLGJADJ_00079) 62347..62877 + 531 WP_011091077 conjugal transfer protein TraQ traQ
SLH58_RS00405 (JBLGJADJ_00080) 62894..63283 + 390 WP_020805690 DUF6750 family protein traR
SLH58_RS00410 (JBLGJADJ_00081) 63329..63823 + 495 WP_223294957 hypothetical protein -
SLH58_RS00415 (JBLGJADJ_00082) 63820..66870 + 3051 WP_020805687 ATPase AAA traU
SLH58_RS00420 (JBLGJADJ_00083) 66900..68075 + 1176 WP_167577398 conjugal transfer protein TraW traW
SLH58_RS00425 (JBLGJADJ_00084) 68072..68722 + 651 WP_032426814 hypothetical protein -
SLH58_RS00430 (JBLGJADJ_00085) 68806..71814 - 3009 WP_047721822 Tn3 family transposase -
SLH58_RS00435 (JBLGJADJ_00086) 71978..72544 + 567 WP_004098835 recombinase family protein -
SLH58_RS00440 (JBLGJADJ_00087) 72626..73279 + 654 WP_205899663 hypothetical protein -
SLH58_RS00445 (JBLGJADJ_00088) 73320..73934 - 615 WP_032640595 TIR domain-containing protein -
SLH58_RS00450 (JBLGJADJ_00089) 74103..74870 + 768 WP_072060054 DUF1883 domain-containing protein -
SLH58_RS00455 (JBLGJADJ_00090) 75005..77119 + 2115 WP_319382210 DotA/TraY family protein traY
SLH58_RS00460 (JBLGJADJ_00091) 77122..77775 + 654 WP_020805693 hypothetical protein -
SLH58_RS00465 (JBLGJADJ_00092) 77837..78079 + 243 WP_023893611 IncL/M type plasmid replication protein RepC -

Host bacterium

ID   20838 GenBank   NZ_OQ821143
Plasmid name   p24A18024_B_KPC Incompatibility group   IncL/M
Plasmid size   78375 bp Coordinate of oriT [Strand]   48846..48951 [+]
Host baterium   Enterobacter hormaechei subsp. steigerwaltii strain 24A18CPO024

Cargo genes

Drug resistance gene   blaKPC-3
Virulence gene   -
Metal resistance gene   merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -