Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120394 |
| Name | oriT_p04AY2016_A_KPC  |
| Organism | Enterobacter hormaechei subsp. steigerwaltii strain 04ACRGNY2016 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OQ821027 (43284..43388 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_p04AY2016_A_KPC
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12834 | GenBank | WP_004206924 |
| Name | traI_SLH58_RS00315_p04AY2016_A_KPC |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 15081 | GenBank | WP_004206885 |
| Name | t4cp2_SLH58_RS00015_p04AY2016_A_KPC |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 46186..65382
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SLH58_RS00295 (EIAKOHGP_00058) | 41270..41581 | + | 312 | WP_004187333 | hypothetical protein | - |
| SLH58_RS00300 (EIAKOHGP_00059) | 41714..42250 | + | 537 | WP_004206920 | hypothetical protein | - |
| SLH58_RS00305 (EIAKOHGP_00061) | 42751..43116 | - | 366 | WP_004206922 | hypothetical protein | - |
| SLH58_RS00310 (EIAKOHGP_00062) | 43392..43709 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| SLH58_RS00315 (EIAKOHGP_00063) | 43696..45675 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| SLH58_RS00320 (EIAKOHGP_00064) | 45689..46189 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| SLH58_RS00325 (EIAKOHGP_00065) | 46186..46965 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| SLH58_RS00330 (EIAKOHGP_00066) | 46976..48139 | + | 1164 | WP_045342066 | plasmid transfer ATPase TraJ | virB11 |
| SLH58_RS00335 (EIAKOHGP_00067) | 48129..48389 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| SLH58_RS00340 (EIAKOHGP_00068) | 48414..51719 | + | 3306 | WP_220035499 | LPD7 domain-containing protein | - |
| SLH58_RS00345 (EIAKOHGP_00069) | 51643..52197 | + | 555 | WP_011154467 | hypothetical protein | traL |
| SLH58_RS00350 (EIAKOHGP_00070) | 52198..52395 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| SLH58_RS00355 (EIAKOHGP_00071) | 52382..52792 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| SLH58_RS00360 (EIAKOHGP_00072) | 52791..53573 | + | 783 | WP_004206929 | DotI/IcmL family type IV secretion protein | traM |
| SLH58_RS00365 (EIAKOHGP_00073) | 53582..54733 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| SLH58_RS00370 (EIAKOHGP_00074) | 54745..56094 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| SLH58_RS00375 (EIAKOHGP_00075) | 56106..56810 | + | 705 | WP_045342064 | conjugal transfer protein TraP | traP |
| SLH58_RS00380 (EIAKOHGP_00076) | 56834..57364 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| SLH58_RS00385 (EIAKOHGP_00077) | 57381..57770 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| SLH58_RS00390 (EIAKOHGP_00078) | 57816..58310 | + | 495 | WP_004187480 | hypothetical protein | - |
| SLH58_RS00395 (EIAKOHGP_00079) | 58307..61357 | + | 3051 | WP_011154474 | conjugative transfer protein | traU |
| SLH58_RS00400 (EIAKOHGP_00080) | 61354..62562 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| SLH58_RS00405 (EIAKOHGP_00081) | 62559..63209 | + | 651 | WP_004187488 | hypothetical protein | - |
| SLH58_RS00410 (EIAKOHGP_00082) | 63202..65382 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| SLH58_RS00415 (EIAKOHGP_00083) | 65385..66038 | + | 654 | WP_004206935 | hypothetical protein | - |
| SLH58_RS00420 (EIAKOHGP_00084) | 66112..66342 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 20823 | GenBank | NZ_OQ821027 |
| Plasmid name | p04AY2016_A_KPC | Incompatibility group | IncL/M |
| Plasmid size | 66638 bp | Coordinate of oriT [Strand] | 43284..43388 [+] |
| Host baterium | Enterobacter hormaechei subsp. steigerwaltii strain 04ACRGNY2016 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |