Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120370 |
| Name | oriT_pECC011a  |
| Organism | Enterobacter cloacae strain ST1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JARXZG010000002 (50667..50753 [-], 87 nt) |
| oriT length | 87 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 87 nt
>oriT_pECC011a
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTAATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTAATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12818 | GenBank | WP_306498446 |
| Name | Relaxase_PTQ60_RS24595_pECC011a |
UniProt ID | _ |
| Length | 899 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 102393.59 Da Isoelectric Point: 8.1586
>WP_306498446.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacter]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVKASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKAKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNHIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWPSLHQRFAKEGLFLTKE
SGELKVKDGWERERPGVTLSAFGHAFDTDKLIRKLGEFVPPAQDIFTQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGGLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIEMLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
SEPHAGSFVSVPADIFERVKPVSRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRMRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGDNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVKASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKAKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNHIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWPSLHQRFAKEGLFLTKE
SGELKVKDGWERERPGVTLSAFGHAFDTDKLIRKLGEFVPPAQDIFTQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGGLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIEMLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
SEPHAGSFVSVPADIFERVKPVSRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRMRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGDNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7235 | GenBank | WP_160251419 |
| Name | WP_160251419_pECC011a |
UniProt ID | _ |
| Length | 111 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 111 a.a. Molecular weight: 12663.63 Da Isoelectric Point: 10.3118
>WP_160251419.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacter]
MSDKASRRGSDKRQKTVIKTLRFTPDEIEEIEQKAEAVGQSVSAYIRNAAMNRKMISLIDDSFMTELLRL
GRLQKHLFVEGKRVGDKEYSTVLVAITELSTTLRKKLMSND
MSDKASRRGSDKRQKTVIKTLRFTPDEIEEIEQKAEAVGQSVSAYIRNAAMNRKMISLIDDSFMTELLRL
GRLQKHLFVEGKRVGDKEYSTVLVAITELSTTLRKKLMSND
Protein domains
No domain identified.
Protein structure
No available structure.
T4CP
| ID | 15061 | GenBank | WP_306498448 |
| Name | trbC_PTQ60_RS24610_pECC011a |
UniProt ID | _ |
| Length | 766 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 766 a.a. Molecular weight: 87702.98 Da Isoelectric Point: 6.8660
>WP_306498448.1 F-type conjugative transfer protein TrbC [Enterobacter cloacae]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKIDPSEDRRVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKIDPSEDRRVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 56876..90886
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PTQ60_RS24600 (PTQ60_24585) | 53892..54095 | - | 204 | WP_133147680 | hypothetical protein | - |
| PTQ60_RS24605 (PTQ60_24590) | 54229..54498 | - | 270 | WP_022650007 | hypothetical protein | - |
| PTQ60_RS24610 (PTQ60_24595) | 54572..56872 | - | 2301 | WP_306498448 | F-type conjugative transfer protein TrbC | - |
| PTQ60_RS24615 (PTQ60_24600) | 56876..58060 | - | 1185 | WP_306498450 | thioredoxin fold domain-containing protein | trbB |
| PTQ60_RS24620 (PTQ60_24605) | 58113..59321 | - | 1209 | WP_032650374 | hypothetical protein | trbA |
| PTQ60_RS24625 (PTQ60_24610) | 59642..60274 | - | 633 | WP_155026669 | plasmid IncI1-type surface exclusion protein ExcA | - |
| PTQ60_RS24630 (PTQ60_24615) | 60336..62495 | - | 2160 | WP_047625721 | DotA/TraY family protein | traY |
| PTQ60_RS24635 (PTQ60_24620) | 62553..63095 | - | 543 | WP_306498453 | conjugal transfer protein TraX | - |
| PTQ60_RS24640 (PTQ60_24625) | 63092..64294 | - | 1203 | WP_032650382 | conjugal transfer protein TraW | traW |
| PTQ60_RS24645 (PTQ60_24630) | 64307..64879 | - | 573 | WP_306498455 | conjugal transfer protein TraV | traV |
| PTQ60_RS24650 (PTQ60_24635) | 64876..67929 | - | 3054 | WP_022649998 | hypothetical protein | traU |
| PTQ60_RS24655 (PTQ60_24640) | 68016..68741 | - | 726 | WP_063864836 | hypothetical protein | traT |
| PTQ60_RS24660 (PTQ60_24645) | 68925..69329 | - | 405 | WP_095427107 | DUF6750 family protein | traR |
| PTQ60_RS24665 (PTQ60_24650) | 69363..69887 | - | 525 | WP_047625719 | conjugal transfer protein TraQ | traQ |
| PTQ60_RS24670 (PTQ60_24655) | 69887..70657 | - | 771 | WP_022649994 | conjugal transfer protein TraP | traP |
| PTQ60_RS24675 (PTQ60_24660) | 70641..71948 | - | 1308 | WP_095427106 | conjugal transfer protein TraO | traO |
| PTQ60_RS24680 (PTQ60_24665) | 71948..72928 | - | 981 | WP_160251424 | DotH/IcmK family type IV secretion protein | traN |
| PTQ60_RS24685 (PTQ60_24670) | 72939..73556 | - | 618 | WP_247607314 | DotI/IcmL family type IV secretion protein | traM |
| PTQ60_RS24690 (PTQ60_24675) | 73646..73996 | - | 351 | WP_022649990 | hypothetical protein | traL |
| PTQ60_RS24695 (PTQ60_24680) | 74014..77556 | - | 3543 | WP_306498457 | LPD7 domain-containing protein | - |
| PTQ60_RS24700 (PTQ60_24685) | 77628..77915 | - | 288 | WP_022649988 | hypothetical protein | traK |
| PTQ60_RS24705 (PTQ60_24690) | 77912..79072 | - | 1161 | WP_229564264 | plasmid transfer ATPase TraJ | virB11 |
| PTQ60_RS24710 (PTQ60_24695) | 79085..79891 | - | 807 | WP_022649986 | type IV secretory system conjugative DNA transfer family protein | traI |
| PTQ60_RS24715 (PTQ60_24700) | 79888..80361 | - | 474 | WP_032650400 | DotD/TraH family lipoprotein | - |
| PTQ60_RS24720 (PTQ60_24705) | 80432..80848 | - | 417 | WP_095427100 | hypothetical protein | - |
| PTQ60_RS24725 (PTQ60_24710) | 80957..82162 | - | 1206 | WP_047625714 | conjugal transfer protein TraF | - |
| PTQ60_RS24730 (PTQ60_24715) | 82268..83104 | - | 837 | WP_306498462 | hypothetical protein | traE |
| PTQ60_RS24735 (PTQ60_24720) | 83251..83727 | - | 477 | WP_075205937 | hypothetical protein | - |
| PTQ60_RS24740 (PTQ60_24725) | 83747..84907 | - | 1161 | WP_306498464 | site-specific integrase | - |
| PTQ60_RS24745 (PTQ60_24730) | 85169..86389 | - | 1221 | WP_306498466 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| PTQ60_RS24750 (PTQ60_24735) | 86386..87060 | - | 675 | WP_306498468 | prepilin peptidase | - |
| PTQ60_RS24755 (PTQ60_24740) | 87045..87527 | - | 483 | WP_045261878 | lytic transglycosylase domain-containing protein | virB1 |
| PTQ60_RS24760 (PTQ60_24745) | 87612..88226 | - | 615 | WP_049222123 | type 4 pilus major pilin | - |
| PTQ60_RS24765 (PTQ60_24750) | 88240..89334 | - | 1095 | WP_049222121 | type II secretion system F family protein | - |
| PTQ60_RS24770 (PTQ60_24755) | 89327..90886 | - | 1560 | WP_306498470 | GspE/PulE family protein | virB11 |
| PTQ60_RS24775 (PTQ60_24760) | 90889..91404 | - | 516 | WP_080298886 | type IV pilus biogenesis protein PilP | - |
| PTQ60_RS24780 (PTQ60_24765) | 91388..92692 | - | 1305 | WP_095427092 | type 4b pilus protein PilO2 | - |
| PTQ60_RS24785 (PTQ60_24770) | 92694..94370 | - | 1677 | WP_047625933 | PilN family type IVB pilus formation outer membrane protein | - |
| PTQ60_RS24790 (PTQ60_24775) | 94385..94822 | - | 438 | WP_022649971 | type IV pilus biogenesis protein PilM | - |
Host bacterium
| ID | 20799 | GenBank | NZ_JARXZG010000002 |
| Plasmid name | pECC011a | Incompatibility group | - |
| Plasmid size | 99848 bp | Coordinate of oriT [Strand] | 50667..50753 [-] |
| Host baterium | Enterobacter cloacae strain ST1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA7 |