Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 120356
• Name: oriT_pRinCIP108029b
• Organism: Rhizobium indigoferae strain CIP 108029
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP140636 (289518..289546 [-], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pRinCIP108029b
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_U5G49_RS25170_pRinCIP108029b

• ID: 12808
• GenBank: WP_322790943
• Name: traA_U5G49_RS25170_pRinCIP108029b
• UniProt ID: _
• Length: 1545 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1545 a.a.
• Molecular weight: 170679.92 Da
• Isoelectric Point: 8.5166

>WP_322790943.1 Ti-type conjugative transfer relaxase TraA [Rhizobium indigoferae]
MAIMFVRAQVIGRGAGRSVVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELALPDQIPAWLRSAISG
QSVAKASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVLDNLTAKGMVADWVYHDKDG
NPHIHLMTTLRPLAEEGFGPKKVPVRGEDGKPLRVVTPDRPNGKVVYKLWGCDKETLQAWKIAWAETASR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRDAEMYFAPADLARRQKMADRLLADPALLLKQ
LSNERSTFDERDIAKALHRTVDDPADFANIRARLMASDDLIMLKPQQIDAETGKVSEPAVFTTREMLRIE
YDMGRSARVLAKRRGFAVSDRVVAAAIRQVETQDPENSFRLDPEQVDAIHHITRDNAIAAVVGLAGAGKS
TLLAAARVAWESGGRRVIGAALAGKAAEGLEDSSGIRSRTLAAWELAWASGRELLERGNVLVIDEAGMVS
SQQMARLLDIAEQAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQPWAREASRLFARGE
VEQGLDAYARHGHLIEAGTRDDVIARIVGDWTEARRQAIRNSTTAGNDGRLRGDELLVLAHTNEDVKRLN
EALRSVMTGEGALSENRAFRTERGARDFAIGDRIIFLENARFVEPRASRLGPQHVKNGMLGTVVSTGDKR
GDTLLSVRLDSGRDVVISESSYRNVDHGYAATIHKSQGATVERSFVLATGMMDQHLTYVSMTRHRDRVDL
YAAKDEFEAKPEWGRKPRVDHAAGVTGALVETGQAKFRPNDEDADDSPYADVRTDDGTLHRLWGVSLPKA
LEDGGVSEGDTVTLRKDGVERVTIKVAIVDEQTGQKRFEEREVDRNVWTAKRIETAAERQQRVERESHRP
DVFKQLVERLSRSGAKTTTLDFESEAGYRAHAQDFARRRGIDSLSQAAAGIEESLSRRWAGIAERREQLA
KLWERASVALGFTIERERRIAYNEERAEPQTALEPDVARGRYLIPPTTGSARSAEEDARLAQLSSPAWKE
REAILRPLLETIYRDPDAALVALNALSSDTSVDPRRLADDLAKAPERLGRLRGSDLIVDGRAARDERNAA
ITALTDLLPLVRAHATQFRRNAERFESHEQQRRAQMALSIPALSEKAMARLTEIEAVRRQGGDDAYKAAF
ALATEDRTIVQEVKAVSEALTARFGWSAFTSKADAVGERNMVERMPEDLASIRGEKLTRLFEAVKRFADE
QHLAERRDRSKSVAGASADQGMETDKENAAVLPMLAAVTEFKTPIDEEARSRALATPLYRHQRAALAAVA
IAIWRDPVGAVGKIEDLLQKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLAAGRERKQAVQAVPEAAA
RLRSLGSAYVNVLDAERQAIAEERRRMAVAIPALSTAAEDVLLRLTAEAKNNDRKLTASAAPLDPSIRRE
FAAVSRALDERFGRNAIIRGEKDLINRVPPAQRNAFEAMQDRLKVLQRTVRMESSELIISERRQRAVSRG
RGIEL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1315-1406)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1127)

• AAA domain (AAA_30) (391-576)

Protein structure

No available structure.

Host bacterium

• ID: 20785
• GenBank: NZ_CP140636
• Plasmid name: pRinCIP108029b
• Incompatibility group: -
• Plasmid size: 576572 bp
• Coordinate of oriT [Strand]: 289518..289546 [-]
• Host baterium: Rhizobium indigoferae strain CIP 108029

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -