Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120290
Name   oriT_pTECL_2-190k-tetX4 in_silico
Organism   Enterobacter cloacae strain TECL_2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MZ773210 (113035..113319 [-], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_pTECL_2-190k-tetX4
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12763 GenBank   WP_039026431
Name   mobH_LZK20_RS00600_pTECL_2-190k-tetX4 insolico UniProt ID   A0A8E6NZY6
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 112997.99 Da        Isoelectric Point: 4.5108

>WP_039026431.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVLRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYSATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure



No available structure.




T4CP

ID   14982 GenBank   WP_000167418
Name   traD_LZK20_RS00605_pTECL_2-190k-tetX4 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77969.74 Da        Isoelectric Point: 8.7275

>WP_000167418.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 176959..186127

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZK20_RS00955 (EHDOMCKP_00204) 172496..172696 - 201 WP_000357614 hypothetical protein -
LZK20_RS00960 (EHDOMCKP_00205) 172714..173790 - 1077 WP_001015069 hypothetical protein -
LZK20_RS00965 (EHDOMCKP_00206) 174325..175200 + 876 WP_001282731 RepB family plasmid replication initiator protein -
LZK20_RS00970 175647..176072 - 426 WP_154166887 hypothetical protein -
LZK20_RS00975 (EHDOMCKP_00208) 176554..176907 + 354 WP_000424120 pili assembly chaperone -
LZK20_RS00980 (EHDOMCKP_00209) 176959..177276 + 318 WP_000203871 type IV conjugative transfer system protein TraL traL
LZK20_RS00985 (EHDOMCKP_00210) 177288..178073 + 786 WP_000771920 TraE/TraK family type IV conjugative transfer system protein traE
LZK20_RS00990 (EHDOMCKP_00211) 178118..179308 + 1191 WP_225374337 type-F conjugative transfer system secretin TraK traK
LZK20_RS00995 (EHDOMCKP_00212) 179310..179750 + 441 WP_001224094 plasmid transfer protein HtdO -
LZK20_RS01000 (EHDOMCKP_00213) 179740..181098 + 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
LZK20_RS01005 (EHDOMCKP_00214) 181107..181619 + 513 WP_000429933 hypothetical protein -
LZK20_RS01010 (EHDOMCKP_00215) 181725..182477 + 753 WP_010892304 protein-disulfide isomerase HtdT -
LZK20_RS01015 (EHDOMCKP_00216) 182487..183437 + 951 WP_001022266 TraV family lipoprotein traV
LZK20_RS01020 (EHDOMCKP_00217) 183446..186127 + 2682 WP_001255540 IncHI-type conjugal transfer ATPase TrhC virb4
LZK20_RS01025 186794..187222 + 429 WP_124061973 hypothetical protein -
LZK20_RS01030 187900..188073 + 174 WP_153801876 hypothetical protein -
LZK20_RS01035 188075..188284 + 210 WP_233340627 hypothetical protein -

Host bacterium

ID   20719 GenBank   NZ_MZ773210
Plasmid name   pTECL_2-190k-tetX4 Incompatibility group   IncFIA
Plasmid size   190185 bp Coordinate of oriT [Strand]   113035..113319 [-]
Host baterium   Enterobacter cloacae strain TECL_2

Cargo genes

Drug resistance gene   tet(X4), floR, qnrS1, blaTEM-1B, ant(3'')-Ia, lnu(G)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -