Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120277
Name   oriT_p3A2 in_silico
Organism   Legionella anisa isolate UMCG_3A
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP029565 (29617..29649 [+], 33 nt)
oriT length   33 nt
IRs (inverted repeats)      3..9, 13..19  (ACGTTGC..GCAACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 33 nt

>oriT_p3A2
GCACGTTGCAAAGCAACGTATAAGCGCGCACTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12755 GenBank   WP_019232995
Name   traA_DLD14_RS19320_p3A2 insolico UniProt ID   _
Length   889 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101374.44 Da        Isoelectric Point: 7.0036

>WP_019232995.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Legionella]
MAIYRFSAQPISRSQGRCVVASSAYRSGEKITDERTGTIYDYTGKNDVVHKEIFLPENAPRWMSERSQLW
NAVELGEHRKDAQLAREVQLALPRELTIEQNTELVREFVQSEFVARGMVADFCFHNPKGDDGLTQPHAHI
LLTLREVNEEGFGLKERAWNDKALLEHWREEWANCQNRHLALHGIDQRVDHRSYKEQGIPLEPQYKIGAK
CAQQHMARLEDHQRIAKENGQLIFERPEIALDALTRNQSTFTHQDLARFINRHTEDAEQFKLVFERVKSC
DELVLLGVDSEGRQRFSTRDMLSIESSMMKQADKLHHQKGHEVDEHALLHAKSNRTLSQEQEVALDYVTS
SGDMKSLLGYAGTGKSYLLGAAREAWEQSGYRVQGAALSGIAALNLSDSSGIESRTIASLFYRLDKGMLT
LNSRDILVVDEAGMLGSRTMERLTREVEKASAKLVLVGDWQQLQAIEAGASFRSIAENYQYAELNQIRRQ
TTPWQVEASFNLAKGDVAHALHAYDIHDHIHRFRTTSEAKHSLIEQWNDVRIAQRCESQIILAYTRKDVK
ELNDMARLIKRRDGELGEDVVFGMERGERAFAVNDRVYFLKREDSLSVINGTLGTIHGIDAKSGVITVAL
DRDDLNKKPQLIQVNTHYYKHMEHGYAATVYKAQGVTVDRTYVLPTKHYDAHSTYVAMTRHRKSCDVFVS
REVFAHDKALVHALNRNRAKDVTLDYTQMASEFARQRGLVSENMPLRSLLKDEHSRKSEPSLSSLMNQVM
GRDVQLEQQKLDAFERQFTKENPSEALHIANSLLSTAAYQAKSLAGLFAPYSKAIARNQLTSVQNEELIR
LIGTLPLDSQVMNAFKKDYPELSTQAELFIKRNEQQLMRIKTIDKELDL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(336-522)

MobA/MobL family (MobA_MobL)

(17-213)


  Protein structure



No available structure.




T4CP

ID   14972 GenBank   WP_019232999
Name   traD_DLD14_RS19300_p3A2 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 70946.29 Da        Isoelectric Point: 7.4043

>WP_019232999.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Legionella]
MSKDPNFKHYTRGGQISFHNLRMWDQITKTLSMICLFLWVVFTVVFAWIFIPLEKITHAVAFYYAQFLSV
IGQKHHFELSFHGKTYKQSVDTILHYAYYQDNANHVINSLGKSAFWAFCLAFILGLGLAYYFIKRGKEQS
DSQYVRGSQLKNSNEVKKKILRDKAHSDIRIDQFPLIRDSEVQHLLVHGTVGTGKSQLIMKIMDCLRKRG
DRVIIYDKGCSFIPHYYQDESDVILNPFDKRCANWDMWLEAPRDSDFENMAESSIPMHGESDPFWVNASR
TVFSCLGSVMRHHSDRSLDKFLKLILTDEFSALEEYLQGTPAATLVSGKIEKTAISIRATLTTYLKSFSA
LAELNQEGKQPFSIRDYILDEQQKGWLFISSNGETHKSLKPLISMWLAQASLALLSLTPDRSRRIWFICD
ELPSLHKLPLLGETIAEVRKFGGCFLLGMQSFSQLTKVYGQAGAKEIFDLLNTRFFFRSPSSDMARLVAA
ELGEEEIEESRENYSYGANSIRDGISLGAQRVTRPIVSYPQIMELKDLNCFVRLPGHYPITQLTLDLGFR
TTKTNGFIERSMPTTFNFSQLTSFDDHLESGTNGHMGSEPLTKNKINATLQCIEELNLEPL

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(35-126)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(171-555)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 7437..25392

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLD14_RS19185 (DLD14_19185) 2803..3336 + 534 WP_019233022 hypothetical protein -
DLD14_RS19190 (DLD14_19190) 3305..4072 - 768 WP_019233021 VUT family protein -
DLD14_RS19195 (DLD14_19195) 4056..4715 - 660 WP_019233020 S24 family peptidase -
DLD14_RS19200 (DLD14_19200) 4914..5156 + 243 WP_019233019 TraY domain-containing protein -
DLD14_RS19205 (DLD14_19205) 5134..6225 + 1092 WP_019233018 hypothetical protein -
DLD14_RS19210 (DLD14_19210) 6270..7013 + 744 WP_019233017 complement resistance protein TraT -
DLD14_RS19215 (DLD14_19215) 7136..7423 + 288 WP_019233016 hypothetical protein -
DLD14_RS19220 (DLD14_19220) 7437..7733 + 297 WP_019233015 type IV conjugative transfer system protein TraL traL
DLD14_RS19225 (DLD14_19225) 7743..8312 + 570 WP_019233014 type IV conjugative transfer system protein TraE traE
DLD14_RS19230 (DLD14_19230) 8305..9024 + 720 WP_019233013 type-F conjugative transfer system secretin TraK traK
DLD14_RS19235 (DLD14_19235) 9017..10384 + 1368 WP_019233012 TrbI/VirB10 family protein traB
DLD14_RS19240 (DLD14_19240) 10381..10671 + 291 WP_019233011 TraV family lipoprotein traV
DLD14_RS19245 (DLD14_19245) 10662..13250 + 2589 WP_019233010 type IV secretion system protein TraC virb4
DLD14_RS19250 (DLD14_19250) 13247..13585 + 339 WP_223499704 type-F conjugative transfer system protein TrbI -
DLD14_RS19255 (DLD14_19255) 13582..14217 + 636 WP_019233008 type-F conjugative transfer system protein TraW traW
DLD14_RS19260 (DLD14_19260) 14207..15190 + 984 WP_019233007 conjugal transfer pilus assembly protein TraU traU
DLD14_RS19265 (DLD14_19265) 15204..15620 + 417 WP_019233006 type-F conjugative transfer system pilin assembly protein TrbC trbC
DLD14_RS19270 (DLD14_19270) 15617..17323 + 1707 WP_019233005 type-F conjugative transfer system mating-pair stabilization protein TraN traN
DLD14_RS19275 (DLD14_19275) 17316..18059 + 744 WP_019233004 type-F conjugative transfer system pilin assembly protein TraF traF
DLD14_RS19280 (DLD14_19280) 18056..18568 + 513 WP_223499702 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
DLD14_RS19285 (DLD14_19285) 18565..19929 + 1365 WP_019233002 conjugal transfer protein TraH traH
DLD14_RS19290 (DLD14_19290) 19941..22772 + 2832 WP_019233001 conjugal transfer mating-pair stabilization protein TraG traG
DLD14_RS19295 (DLD14_19295) 22772..23527 + 756 WP_019233000 hypothetical protein -
DLD14_RS19300 (DLD14_19300) 23527..25392 + 1866 WP_019232999 type IV conjugative transfer system coupling protein TraD virb4
DLD14_RS19305 (DLD14_19305) 25429..25860 - 432 WP_019232998 type II toxin-antitoxin system HigA family antitoxin -
DLD14_RS19310 (DLD14_19310) 25857..26168 - 312 WP_019232997 type II toxin-antitoxin system HigB family toxin -
DLD14_RS19315 (DLD14_19315) 26257..26868 - 612 WP_019232996 hypothetical protein -
DLD14_RS19320 (DLD14_19320) 26879..29548 - 2670 WP_019232995 Ti-type conjugative transfer relaxase TraA -
DLD14_RS19325 (DLD14_19325) 29774..30070 + 297 WP_019232994 hypothetical protein -
DLD14_RS19590 30086..30343 + 258 WP_127908205 hypothetical protein -

Host bacterium

ID   20706 GenBank   NZ_CP029565
Plasmid name   p3A2 Incompatibility group   -
Plasmid size   48353 bp Coordinate of oriT [Strand]   29617..29649 [+]
Host baterium   Legionella anisa isolate UMCG_3A

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1