Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120270
Name   oriT_pKPC-8e09 in_silico
Organism   Aeromonas sp. ASNIH3
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP026225 (69948..70098 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pKPC-8e09
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12749 GenBank   WP_001337757
Name   mobH_C2U39_RS23475_pKPC-8e09 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   14966 GenBank   WP_000178856
Name   traD_C2U39_RS23480_pKPC-8e09 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   14967 GenBank   WP_000637386
Name   traC_C2U39_RS23555_pKPC-8e09 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 68032..93223

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U39_RS23465 (C2U39_23470) 64057..64290 - 234 WP_001191892 hypothetical protein -
C2U39_RS23470 (C2U39_23475) 64272..64889 - 618 WP_001249396 hypothetical protein -
C2U39_RS23475 (C2U39_23480) 65057..68035 + 2979 WP_001337757 MobH family relaxase -
C2U39_RS23480 (C2U39_23485) 68032..69897 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
C2U39_RS23485 (C2U39_23490) 69947..70492 + 546 WP_000228720 hypothetical protein -
C2U39_RS23490 (C2U39_23495) 70449..71078 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
C2U39_RS23495 (C2U39_23500) 71088..71534 + 447 WP_000122506 hypothetical protein -
C2U39_RS23500 (C2U39_23505) 71544..71921 + 378 WP_000869261 hypothetical protein -
C2U39_RS23505 (C2U39_23510) 71921..72583 + 663 WP_001231463 hypothetical protein -
C2U39_RS23510 (C2U39_23515) 72919..73284 + 366 WP_001052531 hypothetical protein -
C2U39_RS23515 (C2U39_23520) 73429..73710 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
C2U39_RS23520 (C2U39_23525) 73707..74333 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
C2U39_RS23525 (C2U39_23530) 74317..75234 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
C2U39_RS23530 (C2U39_23535) 75234..76547 + 1314 WP_024131605 TraB/VirB10 family protein traB
C2U39_RS23535 (C2U39_23540) 76544..77122 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
C2U39_RS23540 (C2U39_23545) 77126..77518 + 393 WP_000479535 TraA family conjugative transfer protein -
C2U39_RS23545 (C2U39_23550) 77719..83250 + 5532 WP_000606833 Ig-like domain-containing protein -
C2U39_RS23550 (C2U39_23555) 83399..84106 + 708 WP_001259347 DsbC family protein -
C2U39_RS23555 (C2U39_23560) 84103..86550 + 2448 WP_000637386 type IV secretion system protein TraC virb4
C2U39_RS23560 (C2U39_23565) 86565..86882 + 318 WP_000351984 hypothetical protein -
C2U39_RS23565 (C2U39_23570) 86879..87409 + 531 WP_001010738 S26 family signal peptidase -
C2U39_RS23570 (C2U39_23575) 87372..88637 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
C2U39_RS23575 (C2U39_23580) 88634..89305 + 672 WP_001337754 EAL domain-containing protein -
C2U39_RS23580 (C2U39_23585) 89305..90309 + 1005 WP_043940352 TraU family protein traU
C2U39_RS23585 (C2U39_23590) 90425..93223 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
C2U39_RS23590 (C2U39_23595) 93262..94122 - 861 WP_000709517 hypothetical protein -
C2U39_RS23595 (C2U39_23600) 94245..94886 - 642 WP_000796664 hypothetical protein -
C2U39_RS23600 (C2U39_23605) 95180..95500 + 321 WP_000547566 hypothetical protein -
C2U39_RS23605 (C2U39_23610) 95804..95989 + 186 WP_001186917 hypothetical protein -
C2U39_RS23610 (C2U39_23615) 96209..97177 + 969 WP_000085162 AAA family ATPase -
C2U39_RS23615 (C2U39_23620) 97188..98096 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   20699 GenBank   NZ_CP026225
Plasmid name   pKPC-8e09 Incompatibility group   IncA/C2
Plasmid size   178635 bp Coordinate of oriT [Strand]   69948..70098 [+]
Host baterium   Aeromonas sp. ASNIH3

Cargo genes

Drug resistance gene   blaFOX-5, blaCARB-2, aadA2, cmlA1, qacE, sul1, qnrA1, msr(E), mph(E), blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -