Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120169 |
| Name | oriT_pKPC_CAV1492  |
| Organism | Serratia marcescens strain CAV1492 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP011639 (64685..65279 [+], 595 nt) |
| oriT length | 595 nt |
| IRs (inverted repeats) | 569..574, 579..584 (ATGCCC..GGGCAT) 497..503, 508..514 (CCTCCCG..CGGGAGG) 433..438, 442..447 (GTTCGC..GCGAAC) 75..80, 86..91 (TGCTTT..AAAGCA) 70..75, 86..91 (TGCTTT..AAAGCA) |
| Location of nic site | 294..295 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 595 nt
>oriT_pKPC_CAV1492
TGTTAAATACATTGTTGTTTTGATAAAATCATTAGTATCTGGCATAACGTCATCATTATCATCTTCTGTTGCTTTGCTTTTAAGTAAAGCACTGGCAAGCAACTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACTTTCAGACATTCAAGTAGATTATCGTGTTGTTTTTCTTCCAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGTGTTTGCCCTATCCTGCATCGCATTAAACTAGCACTACTCTTGAATTGTGCTTCAGCGTCGAAGAAAAAGCAAAACTGAAGGGCTGTTACGGCTGATAGTTTCGCTTGTCTACGACCGGAAACATCTCAATCGCTGTAGGTTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCGCCTCCCGCAAACGGGAGGGCTTCGGCGATTAATGACGTGTGAATCTATTCAGGGTCTGGTGTGTCGCCTCCCATGCCCTGACGGGCATCACACAACCAG
TGTTAAATACATTGTTGTTTTGATAAAATCATTAGTATCTGGCATAACGTCATCATTATCATCTTCTGTTGCTTTGCTTTTAAGTAAAGCACTGGCAAGCAACTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACTTTCAGACATTCAAGTAGATTATCGTGTTGTTTTTCTTCCAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGTGTTTGCCCTATCCTGCATCGCATTAAACTAGCACTACTCTTGAATTGTGCTTCAGCGTCGAAGAAAAAGCAAAACTGAAGGGCTGTTACGGCTGATAGTTTCGCTTGTCTACGACCGGAAACATCTCAATCGCTGTAGGTTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCGCCTCCCGCAAACGGGAGGGCTTCGGCGATTAATGACGTGTGAATCTATTCAGGGTCTGGTGTGTCGCCTCCCATGCCCTGACGGGCATCACACAACCAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12690 | GenBank | WP_015256869 |
| Name | mobP1_AB188_RS00360_pKPC_CAV1492 |
UniProt ID | L0AS04 |
| Length | 386 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 386 a.a. Molecular weight: 44397.89 Da Isoelectric Point: 10.6293
>WP_015256869.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVSVDKEYRVKRKSSESGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQHVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKEHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWKLDNLGVKGIDRKPSSSKELVLN
SAAMLQKQQLQMKQFTQIKQAMLQNEQKMKIGIKLG
MGVSVDKEYRVKRKSSESGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQHVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKEHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWKLDNLGVKGIDRKPSSSKELVLN
SAAMLQKQQLQMKQFTQIKQAMLQNEQKMKIGIKLG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | L0AS04 |
T4CP
| ID | 14896 | GenBank | WP_015256868 |
| Name | t4cp2_AB188_RS00305_pKPC_CAV1492 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69211.90 Da Isoelectric Point: 6.9118
>WP_015256868.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MSLKLPDKGQWLFIGFVMCLFTYYAGSVAVYFLNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAIPA
LLSGLAASVIVPFFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVLLQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPVFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPSLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGVEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYITAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAQKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTKNIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWLFIGFVMCLFTYYAGSVAVYFLNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAIPA
LLSGLAASVIVPFFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVLLQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPVFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPSLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGVEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYITAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAQKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTKNIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 52179..62115
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AB188_RS29225 | 47294..47689 | - | 396 | WP_047365801 | hypothetical protein | - |
| AB188_RS00280 (AB188_00285) | 47700..48290 | - | 591 | WP_015256847 | DUF6012 family protein | - |
| AB188_RS00285 (AB188_00290) | 48303..48695 | - | 393 | WP_227538334 | hypothetical protein | - |
| AB188_RS00290 (AB188_00295) | 48706..49167 | - | 462 | WP_047365817 | thermonuclease family protein | - |
| AB188_RS00295 (AB188_00300) | 49170..49514 | - | 345 | WP_015256850 | hypothetical protein | - |
| AB188_RS29855 | 49606..49944 | - | 339 | WP_227538333 | hypothetical protein | - |
| AB188_RS27670 | 49949..50344 | - | 396 | WP_015256849 | cag pathogenicity island Cag12 family protein | - |
| AB188_RS00305 (AB188_00310) | 50341..52176 | - | 1836 | WP_015256868 | type IV secretory system conjugative DNA transfer family protein | - |
| AB188_RS00310 (AB188_00315) | 52179..53210 | - | 1032 | WP_047727539 | P-type DNA transfer ATPase VirB11 | virB11 |
| AB188_RS00315 (AB188_00320) | 53234..54445 | - | 1212 | WP_015256858 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| AB188_RS00320 (AB188_00325) | 54442..55371 | - | 930 | WP_015256866 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| AB188_RS00325 (AB188_00330) | 55376..56104 | - | 729 | WP_015256865 | virB8 family protein | virB8 |
| AB188_RS00330 (AB188_00335) | 56344..57399 | - | 1056 | WP_015256863 | type IV secretion system protein | virB6 |
| AB188_RS00335 (AB188_00340) | 57411..57665 | - | 255 | WP_015256844 | EexN family lipoprotein | - |
| AB188_RS00340 (AB188_00345) | 57674..58405 | - | 732 | WP_047365797 | type IV secretion system protein | - |
| AB188_RS00345 (AB188_00350) | 58416..61175 | - | 2760 | WP_015256861 | VirB3 family type IV secretion system protein | virb4 |
| AB188_RS00350 (AB188_00355) | 61197..61487 | - | 291 | WP_044715156 | TrbC/VirB2 family protein | virB2 |
| AB188_RS27675 | 61471..62115 | - | 645 | WP_015256857 | lytic transglycosylase domain-containing protein | virB1 |
| AB188_RS00355 (AB188_00360) | 62339..62830 | - | 492 | WP_015256838 | transcription termination/antitermination NusG family protein | - |
| AB188_RS00360 (AB188_00365) | 63186..64346 | - | 1161 | WP_015256869 | MobP1 family relaxase | - |
| AB188_RS00365 (AB188_00370) | 64349..64897 | - | 549 | WP_015256867 | DNA distortion polypeptide 1 | - |
| AB188_RS27690 | 65225..65482 | - | 258 | WP_015256854 | hypothetical protein | - |
| AB188_RS29690 | 65498..65647 | - | 150 | WP_162852120 | hypothetical protein | - |
| AB188_RS29005 | 65725..66066 | - | 342 | WP_015256851 | hypothetical protein | - |
| AB188_RS27695 | 66163..66402 | - | 240 | WP_015256871 | hypothetical protein | - |
| AB188_RS00375 (AB188_00380) | 66395..66661 | - | 267 | WP_015256853 | hypothetical protein | - |
Host bacterium
| ID | 20599 | GenBank | NZ_CP011639 |
| Plasmid name | pKPC_CAV1492 | Incompatibility group | IncX5 |
| Plasmid size | 69158 bp | Coordinate of oriT [Strand] | 64685..65279 [+] |
| Host baterium | Serratia marcescens strain CAV1492 |
Cargo genes
| Drug resistance gene | blaTEM-1A, blaOXA-9, ant(3'')-Ia, aac(6')-Ib, msr(E), mph(E), blaKPC-2, sul1, qacE, dfrA5 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |