Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120165
Name   oriT_pGX1Z-1L in_silico
Organism   Enterobacter cloacae strain GX1Z-1L
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP071862 (60586..60870 [+], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_pGX1Z-1L
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12687 GenBank   WP_039026431
Name   mobH_J4G41_RS23530_pGX1Z-1L insolico UniProt ID   A0A8E6NZY6
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 112997.99 Da        Isoelectric Point: 4.5108

>WP_039026431.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVLRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYSATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure



No available structure.




T4CP

ID   14892 GenBank   WP_000167418
Name   traD_J4G41_RS23525_pGX1Z-1L insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77969.74 Da        Isoelectric Point: 8.7275

>WP_000167418.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 284246..293414

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4G41_RS24695 (J4G41_24665) 279655..280908 - 1254 WP_039026198 ParA family protein -
J4G41_RS24700 (J4G41_24670) 281344..281490 + 147 WP_154166350 hypothetical protein -
J4G41_RS24705 (J4G41_24675) 283151..283573 - 423 WP_144314410 hypothetical protein -
J4G41_RS24710 (J4G41_24680) 284246..286927 - 2682 WP_001255540 IncHI-type conjugal transfer ATPase TrhC virb4
J4G41_RS24715 (J4G41_24685) 286936..287886 - 951 WP_001022266 TraV family lipoprotein traV
J4G41_RS24720 (J4G41_24690) 287896..288648 - 753 WP_010892304 protein-disulfide isomerase HtdT -
J4G41_RS24725 (J4G41_24695) 288754..289266 - 513 WP_000429933 hypothetical protein -
J4G41_RS24730 (J4G41_24700) 289275..290633 - 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
J4G41_RS24735 (J4G41_24705) 290623..291063 - 441 WP_001224094 plasmid transfer protein HtdO -
J4G41_RS24740 (J4G41_24710) 291065..292297 - 1233 WP_000202110 type-F conjugative transfer system secretin TraK traK
J4G41_RS24745 (J4G41_24715) 292300..293085 - 786 WP_000771920 TraE/TraK family type IV conjugative transfer system protein traE
J4G41_RS24750 (J4G41_24720) 293097..293414 - 318 WP_000203871 type IV conjugative transfer system protein TraL traL
J4G41_RS24755 (J4G41_24725) 293466..293819 - 354 WP_000424120 pili assembly chaperone -
J4G41_RS24760 (J4G41_24730) 294382..294726 + 345 WP_153253765 hypothetical protein -
J4G41_RS24765 (J4G41_24735) 295173..296048 - 876 WP_001282731 RepB family plasmid replication initiator protein -

Host bacterium

ID   20595 GenBank   NZ_CP071862
Plasmid name   pGX1Z-1L Incompatibility group   IncHI1B
Plasmid size   296468 bp Coordinate of oriT [Strand]   60586..60870 [+]
Host baterium   Enterobacter cloacae strain GX1Z-1L

Cargo genes

Drug resistance gene   lnu(G), ant(3'')-Ia, blaTEM-1B, qnrS1, tet(A), aph(6)-Id, aph(3'')-Ib, sul2, dfrA14, floR, tet(X4)
Virulence gene   mrkA, mrkB, mrkC, mrkD, mrkF
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -