Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120154 |
| Name | oriT_BB1501_pRMTE4  |
| Organism | Klebsiella quasipneumoniae isolate BB1501 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OV753637 (55062..55167 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_BB1501_pRMTE4
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12683 | GenBank | WP_004187323 |
| Name | traI_OZE11_RS28325_BB1501_pRMTE4 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 14887 | GenBank | WP_004187443 |
| Name | t4cp2_OZE11_RS28060_BB1501_pRMTE4 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79519.87 Da Isoelectric Point: 4.8309
>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 57964..77278
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OZE11_RS28305 (VKR_05643) | 53048..53359 | + | 312 | WP_011154461 | hypothetical protein | - |
| OZE11_RS28310 (VKR_05644) | 53492..54028 | + | 537 | WP_040119830 | hypothetical protein | - |
| OZE11_RS28315 (VKR_05646) | 54529..54924 | - | 396 | WP_170934351 | MobC | - |
| OZE11_RS28320 (VKR_05647) | 54959..55486 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| OZE11_RS28325 (VKR_05648) | 55473..57452 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| OZE11_RS28700 | 57466..57967 | + | 502 | Protein_74 | DotD/TraH family lipoprotein | - |
| OZE11_RS28340 (VKR_05650) | 57964..58743 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| OZE11_RS28345 (VKR_05651) | 58754..59917 | + | 1164 | WP_101976005 | plasmid transfer ATPase TraJ | virB11 |
| OZE11_RS28350 (VKR_05652) | 59907..60167 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| OZE11_RS28355 (VKR_05653) | 60192..63674 | + | 3483 | WP_267668038 | LPD7 domain-containing protein | - |
| OZE11_RS28360 (VKR_05654) | 63640..64152 | + | 513 | WP_011091071 | hypothetical protein | traL |
| OZE11_RS28705 | 64153..64365 | - | 213 | WP_306458688 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| OZE11_RS28370 (VKR_05655) | 64337..64747 | - | 411 | WP_040027863 | H-NS family nucleoid-associated regulatory protein | - |
| OZE11_RS28375 (VKR_05656) | 64815..65528 | + | 714 | WP_223430951 | DotI/IcmL family type IV secretion protein | traM |
| OZE11_RS28380 (VKR_05657) | 65537..66667 | + | 1131 | WP_077255002 | DotH/IcmK family type IV secretion protein | traN |
| OZE11_RS28385 (VKR_05658) | 66679..68028 | + | 1350 | WP_040119748 | conjugal transfer protein TraO | traO |
| OZE11_RS28390 (VKR_05659) | 68040..68744 | + | 705 | WP_040119747 | conjugal transfer protein TraP | traP |
| OZE11_RS28395 (VKR_05660) | 68768..69299 | + | 532 | Protein_86 | conjugal transfer protein TraQ | - |
| OZE11_RS28400 (VKR_05661) | 69316..69705 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| OZE11_RS28405 (VKR_05662) | 69751..70245 | + | 495 | WP_011091080 | hypothetical protein | - |
| OZE11_RS28410 (VKR_05663) | 70242..73292 | + | 3051 | WP_040119746 | conjugal transfer protein | traU |
| OZE11_RS28415 (VKR_05664) | 73289..74497 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| OZE11_RS28420 (VKR_05665) | 74494..75090 | + | 597 | WP_015060003 | hypothetical protein | - |
| OZE11_RS28425 (VKR_05666) | 75083..77278 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| OZE11_RS28430 (VKR_05667) | 77280..77933 | + | 654 | WP_040119745 | hypothetical protein | - |
| OZE11_RS28435 (VKR_05668) | 77997..78245 | + | 249 | WP_015062836 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 20584 | GenBank | NZ_OV753637 |
| Plasmid name | BB1501_pRMTE4 | Incompatibility group | IncL/M |
| Plasmid size | 78541 bp | Coordinate of oriT [Strand] | 55062..55167 [+] |
| Host baterium | Klebsiella quasipneumoniae isolate BB1501 |
Cargo genes
| Drug resistance gene | aac(6')-Ib-cr, blaOXA-1, blaCTX-M-15, blaTEM-1B, rmtE |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |