Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120153
Name   oriT_BB1501_1 in_silico
Organism   Klebsiella quasipneumoniae isolate BB1501
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OV753635 (164316..164364 [+], 49 nt)
oriT length   49 nt
IRs (inverted repeats)      6..13, 16..23  (GCAAAATT..AATTTTGC)
Location of nic site      32..33
Conserved sequence flanking the
  nic site    		  GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 49 nt

>oriT_BB1501_1
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12682 GenBank   WP_267668004
Name   traI_OZE11_RS26830_BB1501_1 insolico UniProt ID   _
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190615.89 Da        Isoelectric Point: 6.5331

>WP_267668004.1 conjugative transfer relaxase/helicase TraI [Klebsiella quasipneumoniae]
MMSIGSVKAAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHKTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGGSVVEFTPKQEKAIQ
KALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1433-1555)

AAA domain (AAA_30)

(971-1157)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

TrwC relaxase (TrwC)

(10-284)


  Protein structure



No available structure.




Auxiliary protein

ID   7151 GenBank   WP_020805752
Name   WP_020805752_BB1501_1 insolico UniProt ID   A0A377TIM3
Length   130 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 130 a.a.        Molecular weight: 14772.81 Da        Isoelectric Point: 4.5715

>WP_020805752.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MAKIQVYVNDNVAEKINAIAVQRRAEGAKEKDISYSSIASMLLELGLRVYEAQMERKESGFNQMAFNRAL
LESMVKTQFTVNKVLGIECLSPHVNGNPKWEWSGLIENIRDDVSAVMEKFFPNESEIEDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-125)


  Protein structure


Source ID Structure
AlphaFold DB A0A377TIM3


T4CP

ID   14886 GenBank   WP_267668005
Name   traD_OZE11_RS26835_BB1501_1 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 86003.08 Da        Isoelectric Point: 5.1202

>WP_267668005.1 type IV conjugative transfer system coupling protein TraD [Klebsiella quasipneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTVWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDEVEIGGNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 136093..164922

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OZE11_RS26835 (VKR_05338) 136093..138405 - 2313 WP_267668005 type IV conjugative transfer system coupling protein TraD virb4
OZE11_RS26840 (VKR_05339) 138532..139221 - 690 WP_013023831 hypothetical protein -
OZE11_RS26845 (VKR_05340) 139414..140143 - 730 Protein_146 conjugal transfer complement resistance protein TraT -
OZE11_RS26850 (VKR_05342) 140329..140862 - 534 WP_014343486 conjugal transfer protein TraS -
OZE11_RS26855 (VKR_05343) 140865..143714 - 2850 WP_013609534 conjugal transfer mating-pair stabilization protein TraG traG
OZE11_RS26860 (VKR_05344) 143714..145093 - 1380 WP_267668006 conjugal transfer pilus assembly protein TraH traH
OZE11_RS26865 (VKR_05345) 145128..145514 - 387 WP_267668007 F-type conjugal transfer protein TrbF -
OZE11_RS26870 (VKR_05346) 145560..146117 - 558 WP_032454975 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
OZE11_RS26875 (VKR_05347) 146089..146328 - 240 WP_009309875 type-F conjugative transfer system pilin chaperone TraQ -
OZE11_RS26880 (VKR_05348) 146339..147091 - 753 WP_009309874 type-F conjugative transfer system pilin assembly protein TraF traF
OZE11_RS26885 (VKR_05349) 147112..147438 - 327 WP_004194451 hypothetical protein -
OZE11_RS26890 (VKR_05350) 147451..147699 - 249 WP_004152675 hypothetical protein -
OZE11_RS26895 (VKR_05351) 147677..147931 - 255 WP_004195500 conjugal transfer protein TrbE -
OZE11_RS26900 (VKR_05352) 147963..149918 - 1956 WP_009309873 type-F conjugative transfer system mating-pair stabilization protein TraN traN
OZE11_RS26905 (VKR_05353) 149977..150615 - 639 WP_004193871 type-F conjugative transfer system pilin assembly protein TrbC trbC
OZE11_RS26910 (VKR_05354) 150628..151617 - 990 WP_009309872 conjugal transfer pilus assembly protein TraU traU
OZE11_RS26915 (VKR_05355) 151614..152003 - 390 WP_004194992 hypothetical protein -
OZE11_RS26920 (VKR_05356) 152045..152671 - 627 WP_004152507 type-F conjugative transfer system protein TraW traW
OZE11_RS26925 (VKR_05357) 152671..153060 - 390 WP_004167468 type-F conjugative transfer system protein TrbI -
OZE11_RS26930 (VKR_05358) 153057..155699 - 2643 WP_032454974 type IV secretion system protein TraC virb4
OZE11_RS26935 (VKR_05359) 155771..156169 - 399 WP_074184101 hypothetical protein -
OZE11_RS26940 (VKR_05360) 156547..156951 - 405 WP_004197817 hypothetical protein -
OZE11_RS26945 (VKR_05361) 157018..157329 - 312 WP_004195240 hypothetical protein -
OZE11_RS26950 (VKR_05362) 157330..157548 - 219 WP_004195235 hypothetical protein -
OZE11_RS26955 (VKR_05363) 157654..158064 - 411 WP_267668008 hypothetical protein -
OZE11_RS26960 (VKR_05364) 158196..158780 - 585 WP_032441881 type IV conjugative transfer system lipoprotein TraV traV
OZE11_RS26965 (VKR_05365) 158894..160318 - 1425 WP_023284634 F-type conjugal transfer pilus assembly protein TraB traB
OZE11_RS26970 (VKR_05366) 160318..161058 - 741 WP_013214019 type-F conjugative transfer system secretin TraK traK
OZE11_RS26975 (VKR_05367) 161045..161611 - 567 WP_004144423 type IV conjugative transfer system protein TraE traE
OZE11_RS26980 (VKR_05368) 161631..161936 - 306 WP_004178059 type IV conjugative transfer system protein TraL traL
OZE11_RS26985 (VKR_05369) 161950..162318 - 369 WP_020316649 type IV conjugative transfer system pilin TraA -
OZE11_RS26990 162387..162587 - 201 WP_050442686 TraY domain-containing protein -
OZE11_RS26995 (VKR_05370) 162671..163375 - 705 WP_050442685 hypothetical protein -
OZE11_RS27000 (VKR_05371) 163613..164005 - 393 WP_020805752 conjugal transfer relaxosome DNA-binding protein TraM -
OZE11_RS27005 (VKR_05372) 164437..164922 + 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
OZE11_RS27010 164955..165284 - 330 WP_011977736 DUF5983 family protein -
OZE11_RS27015 (VKR_05373) 165317..166138 - 822 WP_032454971 DUF932 domain-containing protein -
OZE11_RS27020 (VKR_05374) 166954..167353 - 400 Protein_181 hypothetical protein -

Host bacterium

ID   20583 GenBank   NZ_OV753635
Plasmid name   BB1501_1 Incompatibility group   IncFIB
Plasmid size   207699 bp Coordinate of oriT [Strand]   164316..164364 [+]
Host baterium   Klebsiella quasipneumoniae isolate BB1501

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsC, arsB
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -