Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   120104
Name   oriT_pE512 in_silico
Organism   Enterococcus faecalis strain E512
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP086559 (7949..7998 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)     _
Location of nic site      34..35
Conserved sequence flanking the
  nic site    		  GCTTGCAAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pE512
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12663 GenBank   WP_002415208
Name   Relaxase_LM505_RS15170_pE512 insolico UniProt ID   Q7X5P3
Length   561 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 561 a.a.        Molecular weight: 65380.01 Da        Isoelectric Point: 6.2230

>WP_002415208.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGKYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(37-296)


  Protein structure


Source ID Structure
AlphaFold DB Q7X5P3


Auxiliary protein

ID   5228 GenBank   WP_002393737
Name   WP_002393737_pE512 insolico UniProt ID   A0A8E7CBI6
Length   118 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 13965.01 Da        Isoelectric Point: 9.6545

>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(64-105)


  Protein structure



No available structure.




T4CP

ID   14866 GenBank   WP_002367691
Name   t4cp2_LM505_RS15135_pE512 insolico UniProt ID   _
Length   609 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 609 a.a.        Molecular weight: 70145.14 Da        Isoelectric Point: 6.1807

>WP_002367691.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-558)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 34196..48666

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM505_RS15305 (LM505_15300) 29527..29907 + 381 WP_002367664 hypothetical protein -
LM505_RS15310 (LM505_15305) 29958..30179 + 222 WP_002386480 hypothetical protein -
LM505_RS15315 (LM505_15310) 30481..30819 + 339 WP_229022143 hypothetical protein -
LM505_RS15320 (LM505_15315) 30749..33427 + 2679 WP_087548818 surface exclusion protein SEA1/PrgA -
LM505_RS15325 (LM505_15320) 33571..33906 + 336 WP_002360910 DUF5406 domain-containing protein -
LM505_RS15330 (LM505_15325) 34196..38113 + 3918 WP_087548819 LPXTG-anchored aggregation substance PrgB/Asc10 prgB
LM505_RS15335 (LM505_15330) 38453..38593 + 141 WP_113826368 LPXTG cell wall anchor domain-containing protein prgC
LM505_RS15340 (LM505_15335) 38656..39012 + 357 WP_010711024 pheromone response system RNA-binding regulator PrgU -
LM505_RS15345 (LM505_15340) 39040..39897 + 858 WP_126254887 pheromone response LPXTG-anchored adhesin PrgC prgC
LM505_RS15350 (LM505_15345) 39939..40838 + 900 WP_172692903 type III secretion system protein PrgD -
LM505_RS15355 (LM505_15350) 40858..41292 + 435 WP_002365857 hypothetical protein -
LM505_RS15360 (LM505_15355) 41339..41566 + 228 WP_002365858 hypothetical protein -
LM505_RS15365 (LM505_15360) 41579..41875 + 297 WP_128701256 type III secretion system protein PrgG -
LM505_RS15370 (LM505_15365) 41890..42690 + 801 WP_010774271 type IV secretion system protein prgHb
LM505_RS15375 (LM505_15370) 42692..43045 + 354 WP_002367919 PrgI family mobile element protein prgIc
LM505_RS15380 (LM505_15375) 42999..45389 + 2391 WP_172692904 VirB4-like conjugal transfer ATPase, CD1110 family virb4
LM505_RS15385 (LM505_15380) 45401..48016 + 2616 WP_128701255 phage tail tip lysozyme prgK
LM505_RS15390 (LM505_15385) 48040..48666 + 627 WP_128701254 hypothetical protein prgL

Host bacterium

ID   20534 GenBank   NZ_CP086559
Plasmid name   pE512 Incompatibility group   -
Plasmid size   48881 bp Coordinate of oriT [Strand]   7949..7998 [+]
Host baterium   Enterococcus faecalis strain E512

Cargo genes

Drug resistance gene   -
Virulence gene   prgB/asc10, EF0485
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -