Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120103 |
| Name | oriT_pE512-TC2  |
| Organism | Enterococcus faecalis strain E512-TC2 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP086567 (4475..4524 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 34..35 |
| Conserved sequence flanking the nic site |
GCTTGCAAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pE512-TC2
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12662 | GenBank | WP_029888123 |
| Name | Relaxase_LM512_RS13130_pE512-TC2 |
UniProt ID | _ |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65380.95 Da Isoelectric Point: 6.0136
>WP_029888123.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7145 | GenBank | WP_002393737 |
| Name | WP_002393737_pE512-TC2 |
UniProt ID | A0A8E7CBI6 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 13965.01 Da Isoelectric Point: 9.6545
>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 14865 | GenBank | WP_010717222 |
| Name | t4cp2_LM512_RS13110_pE512-TC2 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70128.19 Da Isoelectric Point: 6.1807
>WP_010717222.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 30884..45354
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LM512_RS13265 (LM512_13265) | 26170..26550 | + | 381 | WP_002367664 | hypothetical protein | - |
| LM512_RS13270 (LM512_13270) | 26601..26822 | + | 222 | WP_002386480 | hypothetical protein | - |
| LM512_RS13275 (LM512_13275) | 27124..27429 | + | 306 | WP_010774969 | hypothetical protein | - |
| LM512_RS13280 (LM512_13280) | 27440..30115 | + | 2676 | WP_174080468 | surface exclusion protein SEA1/PrgA | - |
| LM512_RS13285 (LM512_13285) | 30259..30594 | + | 336 | WP_002360910 | DUF5406 domain-containing protein | - |
| LM512_RS13290 (LM512_13290) | 30884..34801 | + | 3918 | WP_126268646 | LPXTG-anchored aggregation substance | prgB |
| LM512_RS13295 (LM512_13295) | 35141..35281 | + | 141 | WP_113826368 | LPXTG cell wall anchor domain-containing protein | prgC |
| LM512_RS13300 (LM512_13300) | 35344..35700 | + | 357 | WP_010711024 | pheromone response system RNA-binding regulator PrgU | - |
| LM512_RS13305 (LM512_13305) | 35728..36585 | + | 858 | WP_126254887 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| LM512_RS13310 (LM512_13310) | 36627..37526 | + | 900 | WP_172692903 | type III secretion system protein PrgD | - |
| LM512_RS13315 (LM512_13315) | 37546..37980 | + | 435 | WP_002365857 | hypothetical protein | - |
| LM512_RS13320 (LM512_13320) | 38027..38254 | + | 228 | WP_002365858 | hypothetical protein | - |
| LM512_RS13325 (LM512_13325) | 38267..38563 | + | 297 | WP_128701256 | type III secretion system protein PrgG | - |
| LM512_RS13330 (LM512_13330) | 38578..39378 | + | 801 | WP_010774271 | type IV secretion system protein | prgHb |
| LM512_RS13335 (LM512_13335) | 39380..39733 | + | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| LM512_RS13340 (LM512_13340) | 39687..42077 | + | 2391 | WP_172692904 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| LM512_RS13345 (LM512_13345) | 42089..44704 | + | 2616 | WP_128701255 | phage tail tip lysozyme | prgK |
| LM512_RS13350 (LM512_13350) | 44728..45354 | + | 627 | WP_128701254 | hypothetical protein | prgL |
| LM512_RS13355 (LM512_13355) | 45341..45586 | + | 246 | WP_002410289 | hypothetical protein | - |
| LM512_RS13360 (LM512_13360) | 45570..46178 | + | 609 | WP_128701253 | PcfA protein | - |
| LM512_RS13365 (LM512_13365) | 46198..46533 | + | 336 | WP_128701252 | hypothetical protein | - |
| LM512_RS13370 (LM512_13370) | 46603..47358 | + | 756 | WP_016633182 | Fic family protein | - |
| LM512_RS13375 (LM512_13375) | 47375..47833 | + | 459 | WP_116593455 | single-stranded DNA-binding protein | - |
| LM512_RS13380 (LM512_13380) | 47879..48076 | + | 198 | WP_002425087 | hypothetical protein | - |
Host bacterium
| ID | 20533 | GenBank | NZ_CP086567 |
| Plasmid name | pE512-TC2 | Incompatibility group | - |
| Plasmid size | 48589 bp | Coordinate of oriT [Strand] | 4475..4524 [+] |
| Host baterium | Enterococcus faecalis strain E512-TC2 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | prgB/asc10, EF0485 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |