Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120056 |
| Name | oriT_pKqs_09A323_2  |
| Organism | Klebsiella quasipneumoniae subsp. similipneumoniae strain 09A323 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP084785 (54773..54878 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pKqs_09A323_2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12634 | GenBank | WP_059509498 |
| Name | traI_LGM26_RS26445_pKqs_09A323_2 |
UniProt ID | A0A0E3MRA0 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75193.92 Da Isoelectric Point: 9.9948
>WP_059509498.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQIFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQIFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E3MRA0 |
T4CP
| ID | 14834 | GenBank | WP_004187443 |
| Name | t4cp2_LGM26_RS26060_pKqs_09A323_2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79519.87 Da Isoelectric Point: 4.8309
>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 57674..76867
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LGM26_RS26425 (LGM26_26425) | 52759..53070 | + | 312 | WP_004187333 | hypothetical protein | - |
| LGM26_RS26430 (LGM26_26430) | 53203..53739 | + | 537 | WP_011091066 | hypothetical protein | - |
| LGM26_RS26435 (LGM26_26435) | 54240..54635 | - | 396 | WP_031942494 | hypothetical protein | - |
| LGM26_RS26440 (LGM26_26440) | 54670..55197 | + | 528 | WP_224253863 | plasmid mobilization protein MobA | - |
| LGM26_RS26445 (LGM26_26445) | 55184..57163 | + | 1980 | WP_059509498 | TraI/MobA(P) family conjugative relaxase | - |
| LGM26_RS26450 (LGM26_26450) | 57177..57677 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LGM26_RS26455 (LGM26_26455) | 57674..58453 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LGM26_RS26460 (LGM26_26460) | 58464..59627 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LGM26_RS26465 (LGM26_26465) | 59617..59877 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LGM26_RS26470 (LGM26_26470) | 59902..63204 | + | 3303 | WP_031942492 | LPD7 domain-containing protein | - |
| LGM26_RS26475 (LGM26_26475) | 63170..63682 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LGM26_RS26480 (LGM26_26480) | 63683..63895 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LGM26_RS26485 (LGM26_26485) | 63867..64277 | - | 411 | WP_136070593 | H-NS family nucleoid-associated regulatory protein | - |
| LGM26_RS26490 (LGM26_26490) | 64345..65058 | + | 714 | WP_109914255 | DotI/IcmL family type IV secretion protein | traM |
| LGM26_RS26495 (LGM26_26495) | 65067..66218 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| LGM26_RS26500 (LGM26_26500) | 66230..67579 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| LGM26_RS26505 (LGM26_26505) | 67591..68295 | + | 705 | WP_031942491 | conjugal transfer protein TraP | traP |
| LGM26_RS26510 (LGM26_26510) | 68319..68849 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| LGM26_RS26515 (LGM26_26515) | 68866..69255 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| LGM26_RS26520 (LGM26_26520) | 69301..69795 | + | 495 | WP_011091080 | hypothetical protein | - |
| LGM26_RS26525 (LGM26_26525) | 69792..72842 | + | 3051 | WP_031942490 | plasmid transfer protein | traU |
| LGM26_RS26530 (LGM26_26530) | 72839..74047 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| LGM26_RS26535 (LGM26_26535) | 74044..74694 | + | 651 | WP_004187488 | hypothetical protein | - |
| LGM26_RS26540 (LGM26_26540) | 74687..76867 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| LGM26_RS26545 (LGM26_26545) | 76870..77523 | + | 654 | WP_004206935 | hypothetical protein | - |
| LGM26_RS26550 (LGM26_26550) | 77597..77827 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 20486 | GenBank | NZ_CP084785 |
| Plasmid name | pKqs_09A323_2 | Incompatibility group | IncL/M |
| Plasmid size | 78123 bp | Coordinate of oriT [Strand] | 54773..54878 [+] |
| Host baterium | Klebsiella quasipneumoniae subsp. similipneumoniae strain 09A323 |
Cargo genes
| Drug resistance gene | blaSHV-5, ant(3'')-Ia, aac(3)-Ia |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |