Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 120014 |
| Name | oriT_pKqs_12A476_2  |
| Organism | Klebsiella quasipneumoniae subsp. similipneumoniae strain 12A476 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP084781 (87238..87287 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
TGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKqs_12A476_2
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 14814 | GenBank | WP_040215031 |
| Name | traD_LGM27_RS26675_pKqs_12A476_2 |
UniProt ID | _ |
| Length | 760 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 760 a.a. Molecular weight: 85020.03 Da Isoelectric Point: 4.9634
>WP_040215031.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLTMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDERVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSPPA
PAEMTVSPAPVKAPPTTKRPAAEPPVLPVTPIPLLKPKAAAAAATASSAGTPAAAAGGTEQELAQQSAEQ
GQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNI
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLTMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDERVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSPPA
PAEMTVSPAPVKAPPTTKRPAAEPPVLPVTPIPLLKPKAAAAAATASSAGTPAAAAGGTEQELAQQSAEQ
GQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 86680..119428
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LGM27_RS26450 (LGM27_26450) | 81782..82132 | + | 351 | WP_004153414 | hypothetical protein | - |
| LGM27_RS26455 (LGM27_26455) | 82762..83115 | + | 354 | WP_004152748 | hypothetical protein | - |
| LGM27_RS26460 (LGM27_26460) | 83172..83519 | + | 348 | WP_004152749 | hypothetical protein | - |
| LGM27_RS26465 (LGM27_26465) | 83614..83760 | + | 147 | WP_004152750 | hypothetical protein | - |
| LGM27_RS26470 (LGM27_26470) | 83811..84644 | + | 834 | WP_004152751 | N-6 DNA methylase | - |
| LGM27_RS26475 (LGM27_26475) | 85464..86285 | + | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| LGM27_RS26480 (LGM27_26480) | 86318..86647 | + | 330 | WP_011977736 | DUF5983 family protein | - |
| LGM27_RS26485 (LGM27_26485) | 86680..87165 | - | 486 | WP_022631514 | transglycosylase SLT domain-containing protein | virB1 |
| LGM27_RS26490 (LGM27_26490) | 87556..87972 | + | 417 | WP_168429831 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| LGM27_RS26495 (LGM27_26495) | 88172..88891 | + | 720 | WP_162920041 | conjugal transfer protein TrbJ | - |
| LGM27_RS26500 (LGM27_26500) | 89024..89227 | + | 204 | WP_171486139 | TraY domain-containing protein | - |
| LGM27_RS26505 (LGM27_26505) | 89292..89660 | + | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| LGM27_RS26510 (LGM27_26510) | 89674..89979 | + | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| LGM27_RS26515 (LGM27_26515) | 89999..90565 | + | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| LGM27_RS26520 (LGM27_26520) | 90552..91292 | + | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| LGM27_RS26525 (LGM27_26525) | 91292..92716 | + | 1425 | WP_032426793 | F-type conjugal transfer pilus assembly protein TraB | traB |
| LGM27_RS26530 (LGM27_26530) | 92830..93414 | + | 585 | WP_004161368 | type IV conjugative transfer system lipoprotein TraV | traV |
| LGM27_RS26535 (LGM27_26535) | 93546..93956 | + | 411 | WP_004152499 | hypothetical protein | - |
| LGM27_RS26540 (LGM27_26540) | 94062..94280 | + | 219 | WP_122008102 | hypothetical protein | - |
| LGM27_RS26545 (LGM27_26545) | 94281..94592 | + | 312 | WP_004152502 | hypothetical protein | - |
| LGM27_RS26550 (LGM27_26550) | 94659..95063 | + | 405 | WP_004152503 | hypothetical protein | - |
| LGM27_RS26555 (LGM27_26555) | 95106..95495 | + | 390 | WP_004153076 | hypothetical protein | - |
| LGM27_RS26560 (LGM27_26560) | 95503..95901 | + | 399 | WP_011977783 | hypothetical protein | - |
| LGM27_RS26565 (LGM27_26565) | 95973..98612 | + | 2640 | WP_004152505 | type IV secretion system protein TraC | virb4 |
| LGM27_RS26570 (LGM27_26570) | 98612..99001 | + | 390 | WP_004152506 | type-F conjugative transfer system protein TrbI | - |
| LGM27_RS26575 (LGM27_26575) | 99001..99627 | + | 627 | WP_032420974 | type-F conjugative transfer system protein TraW | traW |
| LGM27_RS26580 (LGM27_26580) | 99669..100058 | + | 390 | WP_032445235 | hypothetical protein | - |
| LGM27_RS26585 (LGM27_26585) | 100055..101044 | + | 990 | WP_011977785 | conjugal transfer pilus assembly protein TraU | traU |
| LGM27_RS26590 (LGM27_26590) | 101057..101695 | + | 639 | WP_004193871 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| LGM27_RS26595 (LGM27_26595) | 101754..103709 | + | 1956 | WP_048292285 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| LGM27_RS26600 (LGM27_26600) | 103741..103995 | + | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| LGM27_RS26605 (LGM27_26605) | 103973..104221 | + | 249 | WP_004152675 | hypothetical protein | - |
| LGM27_RS26610 (LGM27_26610) | 104234..104560 | + | 327 | WP_004144402 | hypothetical protein | - |
| LGM27_RS26615 (LGM27_26615) | 104581..105333 | + | 753 | WP_020803149 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| LGM27_RS26620 (LGM27_26620) | 105344..105583 | + | 240 | WP_004152687 | type-F conjugative transfer system pilin chaperone TraQ | - |
| LGM27_RS26625 (LGM27_26625) | 105555..106127 | + | 573 | WP_023292147 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| LGM27_RS26630 (LGM27_26630) | 106120..106548 | + | 429 | WP_015065560 | hypothetical protein | - |
| LGM27_RS26635 (LGM27_26635) | 106535..107905 | + | 1371 | WP_004159274 | conjugal transfer pilus assembly protein TraH | traH |
| LGM27_RS26640 (LGM27_26640) | 107905..110748 | + | 2844 | WP_032420975 | conjugal transfer mating-pair stabilization protein TraG | traG |
| LGM27_RS26645 (LGM27_26645) | 110759..111283 | + | 525 | WP_015065538 | hypothetical protein | - |
| LGM27_RS26650 (LGM27_26650) | 111642..112667 | + | 1026 | WP_064782720 | IS110 family transposase | - |
| LGM27_RS26655 (LGM27_26655) | 112986..113954 | - | 969 | WP_072193976 | IS5-like element IS903B family transposase | - |
| LGM27_RS26660 (LGM27_26660) | 114249..115274 | - | 1026 | WP_064782720 | IS110 family transposase | - |
| LGM27_RS26665 (LGM27_26665) | 115406..116137 | + | 732 | WP_013023830 | conjugal transfer complement resistance protein TraT | - |
| LGM27_RS26670 (LGM27_26670) | 116330..117019 | + | 690 | WP_165457226 | hypothetical protein | - |
| LGM27_RS26675 (LGM27_26675) | 117146..119428 | + | 2283 | WP_040215031 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 20444 | GenBank | NZ_CP084781 |
| Plasmid name | pKqs_12A476_2 | Incompatibility group | IncFIA |
| Plasmid size | 130593 bp | Coordinate of oriT [Strand] | 87238..87287 [-] |
| Host baterium | Klebsiella quasipneumoniae subsp. similipneumoniae strain 12A476 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |