Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119997
Name   oriT_pWW14A-IncFII/IncR in_silico
Organism   Klebsiella quasipneumoniae strain WW-14A
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP080100 (16817..16866 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  GGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pWW14A-IncFII/IncR
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure



Relaxase

ID   12604 GenBank   WP_130973550
Name   traI_KZX49_RS27360_pWW14A-IncFII/IncR insolico UniProt ID   _
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190778.77 Da        Isoelectric Point: 6.1566

>WP_130973550.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-284)

DNA helicase TraI (TraI_C)

(1433-1555)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

AAA domain (AAA_30)

(971-1157)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)


  Protein structure



No available structure.




T4CP

ID   14803 GenBank   WP_048235023
Name   traD_KZX49_RS27355_pWW14A-IncFII/IncR insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85941.02 Da        Isoelectric Point: 5.1149

>WP_048235023.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRSTEPSALRLTTVPLIKPKAAAAAAAAATVSSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDNVEIGGNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 16259..44876

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX49_RS27160 (KZX49_27140) 11483..12715 + 1233 WP_032249456 hypothetical protein -
KZX49_RS27165 (KZX49_27145) 12849..13340 + 492 WP_032249453 hypothetical protein -
KZX49_RS27170 (KZX49_27150) 14356..15336 + 981 WP_000082736 IS5-like element ISEc68 family transposase -
KZX49_RS27175 (KZX49_27155) 15484..15870 + 387 Protein_19 DUF932 domain-containing protein -
KZX49_RS27180 (KZX49_27160) 15897..16226 + 330 WP_011977736 DUF5983 family protein -
KZX49_RS27185 (KZX49_27165) 16259..16744 - 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
KZX49_RS27190 (KZX49_27170) 17179..17571 + 393 WP_032436755 conjugal transfer relaxosome DNA-binding protein TraM -
KZX49_RS27195 (KZX49_27175) 17778..18473 + 696 WP_023307551 transcriptional regulator TraJ family protein -
KZX49_RS27200 (KZX49_27180) 18557..18928 + 372 WP_004208838 TraY domain-containing protein -
KZX49_RS27205 (KZX49_27185) 18982..19350 + 369 WP_023307550 type IV conjugative transfer system pilin TraA -
KZX49_RS27210 (KZX49_27190) 19364..19669 + 306 WP_004178059 type IV conjugative transfer system protein TraL traL
KZX49_RS27215 (KZX49_27195) 19689..20255 + 567 WP_004152602 type IV conjugative transfer system protein TraE traE
KZX49_RS27220 (KZX49_27200) 20242..20982 + 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
KZX49_RS27225 (KZX49_27205) 20982..22406 + 1425 WP_023307549 F-type conjugal transfer pilus assembly protein TraB traB
KZX49_RS27230 (KZX49_27210) 22399..22818 + 420 Protein_30 conjugal transfer pilus-stabilizing protein TraP -
KZX49_RS27235 (KZX49_27215) 23030..23599 + 570 WP_023307547 type IV conjugative transfer system lipoprotein TraV traV
KZX49_RS27240 (KZX49_27220) 23731..24141 + 411 WP_023307546 hypothetical protein -
KZX49_RS27245 (KZX49_27225) 24146..24436 + 291 WP_032423381 hypothetical protein -
KZX49_RS27250 (KZX49_27230) 24460..24678 + 219 WP_004171484 hypothetical protein -
KZX49_RS27255 (KZX49_27235) 24679..24996 + 318 WP_023307544 hypothetical protein -
KZX49_RS27260 (KZX49_27240) 25063..25467 + 405 WP_023307543 hypothetical protein -
KZX49_RS27265 (KZX49_27245) 25464..25754 + 291 WP_023307542 hypothetical protein -
KZX49_RS27270 (KZX49_27250) 25762..26160 + 399 WP_072158599 hypothetical protein -
KZX49_RS27275 (KZX49_27255) 26232..28871 + 2640 WP_016528865 type IV secretion system protein TraC virb4
KZX49_RS27280 (KZX49_27260) 28871..29260 + 390 WP_032436749 type-F conjugative transfer system protein TrbI -
KZX49_RS27285 (KZX49_27265) 29260..29886 + 627 WP_023307538 type-F conjugative transfer system protein TraW traW
KZX49_RS27290 (KZX49_27270) 29930..30889 + 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
KZX49_RS27295 (KZX49_27275) 30902..31540 + 639 WP_015065635 type-F conjugative transfer system pilin assembly protein TrbC trbC
KZX49_RS27300 (KZX49_27280) 31599..33554 + 1956 WP_023307537 type-F conjugative transfer system mating-pair stabilization protein TraN traN
KZX49_RS27305 (KZX49_27285) 33586..33822 + 237 WP_032436746 conjugal transfer protein TrbE -
KZX49_RS30285 33819..34004 + 186 WP_032436745 hypothetical protein -
KZX49_RS27310 (KZX49_27290) 34050..34376 + 327 WP_032436744 hypothetical protein -
KZX49_RS27315 (KZX49_27295) 34397..35149 + 753 WP_015065637 type-F conjugative transfer system pilin assembly protein TraF traF
KZX49_RS27320 (KZX49_27300) 35160..35399 + 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
KZX49_RS27325 (KZX49_27305) 35371..35928 + 558 WP_004152678 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
KZX49_RS27330 (KZX49_27310) 35973..36416 + 444 WP_032436741 F-type conjugal transfer protein TrbF -
KZX49_RS27335 (KZX49_27315) 36394..37773 + 1380 WP_072158600 conjugal transfer pilus assembly protein TraH traH
KZX49_RS27340 (KZX49_27320) 37773..40595 + 2823 WP_023320108 conjugal transfer mating-pair stabilization protein TraG traG
KZX49_RS27345 (KZX49_27325) 40619..41221 + 603 WP_023280878 hypothetical protein -
KZX49_RS27350 (KZX49_27330) 41473..42204 + 732 WP_032415736 conjugal transfer complement resistance protein TraT -
KZX49_RS30010 42395..42508 + 114 Protein_56 hypothetical protein -
KZX49_RS27355 (KZX49_27335) 42564..44876 + 2313 WP_048235023 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   20427 GenBank   NZ_CP080100
Plasmid name   pWW14A-IncFII/IncR Incompatibility group   IncFII
Plasmid size   228495 bp Coordinate of oriT [Strand]   16817..16866 [-]
Host baterium   Klebsiella quasipneumoniae strain WW-14A

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9