Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 119904 |
| Name | oriT_pNTT31XS-tetX4  |
| Organism | Klebsiella aerogenes strain NTT31XS |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP077430 (53357..53438 [-], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_pNTT31XS-tetX4
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12567 | GenBank | WP_032440569 |
| Name | traI_KSL85_RS25170_pNTT31XS-tetX4 |
UniProt ID | A0A7G3L4G1 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74384.24 Da Isoelectric Point: 8.3203
>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7G3L4G1 |
T4CP
| ID | 14763 | GenBank | WP_032440593 |
| Name | trbC_KSL85_RS25035_pNTT31XS-tetX4 |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82679.78 Da Isoelectric Point: 6.3201
>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 22315..44248
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KSL85_RS25020 (KSL85_25020) | 18078..18704 | - | 627 | WP_254913868 | RepA family replication protein | - |
| KSL85_RS25025 (KSL85_25025) | 19279..19530 | - | 252 | WP_113394424 | hypothetical protein | - |
| KSL85_RS25030 (KSL85_25030) | 19542..20075 | - | 534 | WP_032440594 | phospholipase D family protein | - |
| KSL85_RS25035 (KSL85_25035) | 20121..22313 | - | 2193 | WP_032440593 | F-type conjugative transfer protein TrbC | - |
| KSL85_RS25040 (KSL85_25040) | 22315..23436 | - | 1122 | WP_052455213 | thioredoxin fold domain-containing protein | trbB |
| KSL85_RS25045 (KSL85_25045) | 23417..24835 | - | 1419 | WP_063666705 | hypothetical protein | trbA |
| KSL85_RS25050 (KSL85_25050) | 24937..25560 | - | 624 | WP_032440592 | plasmid IncI1-type surface exclusion protein ExcA | - |
| KSL85_RS25055 (KSL85_25055) | 25626..27770 | - | 2145 | WP_032440591 | DotA/TraY family protein | traY |
| KSL85_RS25060 (KSL85_25060) | 27812..28393 | - | 582 | WP_050597335 | conjugal transfer protein TraX | - |
| KSL85_RS25065 (KSL85_25065) | 28390..29586 | - | 1197 | WP_032440590 | conjugal transfer protein TraW | traW |
| KSL85_RS25070 (KSL85_25070) | 29596..32643 | - | 3048 | WP_032440589 | conjugal transfer protein | traU |
| KSL85_RS25075 (KSL85_25075) | 32653..32967 | - | 315 | WP_050597334 | hypothetical protein | traT |
| KSL85_RS25080 (KSL85_25080) | 33260..33661 | - | 402 | WP_032440588 | DUF6750 family protein | traR |
| KSL85_RS25085 (KSL85_25085) | 33717..34250 | - | 534 | WP_032440587 | conjugal transfer protein TraQ | traQ |
| KSL85_RS25090 (KSL85_25090) | 34260..34979 | - | 720 | WP_050597333 | conjugal transfer protein TraP | traP |
| KSL85_RS25095 (KSL85_25095) | 34986..36326 | - | 1341 | WP_032440586 | conjugal transfer protein TraO | traO |
| KSL85_RS25100 (KSL85_25100) | 36330..37358 | - | 1029 | WP_204361180 | DotH/IcmK family type IV secretion protein | traN |
| KSL85_RS25105 (KSL85_25105) | 37444..38112 | - | 669 | WP_032440585 | DotI/IcmL/TraM family protein | traM |
| KSL85_RS25110 (KSL85_25110) | 38184..38537 | - | 354 | WP_032440584 | hypothetical protein | traL |
| KSL85_RS25115 (KSL85_25115) | 38571..41984 | - | 3414 | WP_052145516 | LPD7 domain-containing protein | - |
| KSL85_RS25120 (KSL85_25120) | 42002..42268 | - | 267 | WP_032440577 | IcmT/TraK family protein | traK |
| KSL85_RS25125 (KSL85_25125) | 42265..43407 | - | 1143 | WP_032440582 | plasmid transfer ATPase TraJ | virB11 |
| KSL85_RS25130 (KSL85_25130) | 43430..44248 | - | 819 | WP_032440576 | type IV secretory system conjugative DNA transfer family protein | traI |
| KSL85_RS25135 (KSL85_25135) | 44245..44709 | - | 465 | WP_225546581 | DotD/TraH family lipoprotein | - |
| KSL85_RS25140 (KSL85_25140) | 44735..45214 | - | 480 | WP_032440574 | lytic transglycosylase domain-containing protein | - |
| KSL85_RS25145 (KSL85_25145) | 45946..46173 | - | 228 | WP_032440573 | hypothetical protein | - |
| KSL85_RS25150 (KSL85_25150) | 46783..47625 | + | 843 | WP_032440572 | helix-turn-helix domain-containing protein | - |
| KSL85_RS25155 (KSL85_25155) | 47685..48614 | + | 930 | WP_032440581 | glycosyltransferase family 9 protein | - |
| KSL85_RS25160 (KSL85_25160) | 48607..49101 | + | 495 | WP_032440571 | molybdopterin-dependent oxidoreductase | - |
Host bacterium
| ID | 20334 | GenBank | NZ_CP077430 |
| Plasmid name | pNTT31XS-tetX4 | Incompatibility group | IncFII |
| Plasmid size | 78159 bp | Coordinate of oriT [Strand] | 53357..53438 [-] |
| Host baterium | Klebsiella aerogenes strain NTT31XS |
Cargo genes
| Drug resistance gene | tet(X4) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |