Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 119872 |
| Name | oriT_pmk2  |
| Organism | Klebsiella sp. PL-2018 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP077675 (94996..95075 [-], 80 nt) |
| oriT length | 80 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 80 nt
>oriT_pmk2
GGGGTGTCGGGGCAAAGCCCTGACCAGAGGGTAATTTAATGCTGTTGCGTGCGACGGTATTACAATTACACATCCTGTCC
GGGGTGTCGGGGCAAAGCCCTGACCAGAGGGTAATTTAATGCTGTTGCGTGCGACGGTATTACAATTACACATCCTGTCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12550 | GenBank | WP_217077015 |
| Name | Relaxase_MKleb_RS28890_pmk2 |
UniProt ID | _ |
| Length | 897 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 897 a.a. Molecular weight: 102217.14 Da Isoelectric Point: 7.4985
>WP_217077015.1 relaxase/mobilization nuclease domain-containing protein [Klebsiella sp. PL-2018]
MIPVIPPKRQDGKSSFEDLVSYVAVRDDVVDDDLKAVVEKGRNAPHRNRFSRLVDYATRLREGAFVELVD
TMKDGCEWINFYGVTCFHNCSSLETAAQDMAYTADKAHFGGDDSDPVFHYILSWQSHESPHSEQIYDSVR
HTLKALGLSSHQYMSAVHTDTDNLHVHVAVNRVHPETGYYNCLSWSKEKLNRACRELELKHGFAPDNGCY
VIAPDKRIVRRTGAERNRQSGWTRGKPQTFQAYIADTAIMGLREAPVTDWTSLHQRFAREGLYLAMTEGR
LEVKDGWVQTRAGVDLSGFGPSWSLDRLTQRLGAWTPPAQDIFQKTPDVGRYDLTRVPPITRPEVMQVNQ
TLAEYATEHLRSSLAWIDAEPSRRRIGMIHGLLAQSGLFLKEQNDGLVICDGFSPGRTPVRAEYVWPVLT
KPLLSTYEGGWRPVPSDIFQQIKPARQYTGGELVPAPATDERWLKMRTGTGPAGALKREIFSDKESLWGY
AASHCRLDLDNLIIDGQFTWQGCHELLARQGLLLRRQHQGLAVVDAYHPRLTGVKASHVHPDLTLARAEP
YAGPFEEVAADIFERVQITHRYDPERAVSDRDIPSMKRDPELRRQRREARAAARQDLRARYELWREGWRK
PDLRYGERSGEIHQACRMKKAHARVQFRDPLVRKLHYHIAEVQRMQALIALKQSVREERLTLVDAGKWYP
PTFRQWVAGEALKGDKAAISQLRGWDYRSRRNPAASRTTDSQCVVICEPGGEPAYNNFPDSGLHATLKHN
GCVQFTNEQAGEFICSDYGDRVVFRNHEDYETLKNVLVKTSPVLFSRNDSVEMSPEGEDEPFNTAFAKMV
AWRNVTAGDGLEYRVARPTLDRLRIEQEERYQDMLNPRTYHAGPVEDERPGWTPPSP
MIPVIPPKRQDGKSSFEDLVSYVAVRDDVVDDDLKAVVEKGRNAPHRNRFSRLVDYATRLREGAFVELVD
TMKDGCEWINFYGVTCFHNCSSLETAAQDMAYTADKAHFGGDDSDPVFHYILSWQSHESPHSEQIYDSVR
HTLKALGLSSHQYMSAVHTDTDNLHVHVAVNRVHPETGYYNCLSWSKEKLNRACRELELKHGFAPDNGCY
VIAPDKRIVRRTGAERNRQSGWTRGKPQTFQAYIADTAIMGLREAPVTDWTSLHQRFAREGLYLAMTEGR
LEVKDGWVQTRAGVDLSGFGPSWSLDRLTQRLGAWTPPAQDIFQKTPDVGRYDLTRVPPITRPEVMQVNQ
TLAEYATEHLRSSLAWIDAEPSRRRIGMIHGLLAQSGLFLKEQNDGLVICDGFSPGRTPVRAEYVWPVLT
KPLLSTYEGGWRPVPSDIFQQIKPARQYTGGELVPAPATDERWLKMRTGTGPAGALKREIFSDKESLWGY
AASHCRLDLDNLIIDGQFTWQGCHELLARQGLLLRRQHQGLAVVDAYHPRLTGVKASHVHPDLTLARAEP
YAGPFEEVAADIFERVQITHRYDPERAVSDRDIPSMKRDPELRRQRREARAAARQDLRARYELWREGWRK
PDLRYGERSGEIHQACRMKKAHARVQFRDPLVRKLHYHIAEVQRMQALIALKQSVREERLTLVDAGKWYP
PTFRQWVAGEALKGDKAAISQLRGWDYRSRRNPAASRTTDSQCVVICEPGGEPAYNNFPDSGLHATLKHN
GCVQFTNEQAGEFICSDYGDRVVFRNHEDYETLKNVLVKTSPVLFSRNDSVEMSPEGEDEPFNTAFAKMV
AWRNVTAGDGLEYRVARPTLDRLRIEQEERYQDMLNPRTYHAGPVEDERPGWTPPSP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7104 | GenBank | WP_217077014 |
| Name | WP_217077014_pmk2 |
UniProt ID | _ |
| Length | 110 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 110 a.a. Molecular weight: 12663.70 Da Isoelectric Point: 10.6395
>WP_217077014.1 plasmid mobilization protein MobA [Klebsiella sp. PL-2018]
MSDVSKKKKSETRQKTVVRTLRLSSSEDNDIRQKAEKSGMPVSTYLRKAALEKQVNARIDDTFLMELMRL
GRMQKHLFVEGKRTGDKEYASVLVAITELTTTLRNQLMEK
MSDVSKKKKSETRQKTVVRTLRLSSSEDNDIRQKAEKSGMPVSTYLRKAALEKQVNARIDDTFLMELMRL
GRMQKHLFVEGKRTGDKEYASVLVAITELTTTLRNQLMEK
Protein domains
No domain identified.
Protein structure
No available structure.
Host bacterium
| ID | 20302 | GenBank | NZ_CP077675 |
| Plasmid name | pmk2 | Incompatibility group | - |
| Plasmid size | 122124 bp | Coordinate of oriT [Strand] | 94996..95075 [-] |
| Host baterium | Klebsiella sp. PL-2018 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | wzi |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |