Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119871
Name   oriT_pmk1 in_silico
Organism   Klebsiella sp. PL-2018
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP077674 (102804..102883 [+], 80 nt)
oriT length   80 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 80 nt

>oriT_pmk1
GGGGTGTCGGGGCAAAGCCCTGACCAGAGGGTAATTTAATGCTGTCGCGTGCGACGGTATTACAATTACACATCCTGTCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12549 GenBank   WP_217076845
Name   Relaxase_MKleb_RS28065_pmk1 insolico UniProt ID   _
Length   898 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 898 a.a.        Molecular weight: 102632.84 Da        Isoelectric Point: 8.6275

>WP_217076845.1 relaxase/mobilization nuclease domain-containing protein [Klebsiella sp. PL-2018]
MIPVIPPKRQDGKSSFEDLVSYVSVRDDVVDDDLKAVVEKGRNAPHRNRFSRLVDYATRLREGAFVELVD
TMKDGCEWINFYGVTCFHNCSSLETAAQDMAYTADKAHFGGDDSDPVFHYILSWQSHESPHPEQIYDSVR
HTLKALGLSSHQYMSAVHTDTDNLHVHVAVNRVHPETGYYNCLSWSKEKLNRACRELELKHGFAPDNGCY
VIAPDKRIVRRTGAERNRQSGWTRGKPQTFQAYIADTAIMGLREAPVTDWASLHQRFAREGLYLAMTEGR
LEVKDGWVQTRAGVDLSGFGPSWSLDRLTQRLGAWTPPAQDIFQQTPDVGRYDLTRVPPITRPEVMQVNQ
TLAEYATEHLRSSLAWIDAEPSRRRIGMIHGLLAQSGLFLKEQNDGLVICDGFSPGRTPVRAEYVWPVLT
KPLLSTYEGGWRPVPSDIFQQIKPARQYTGGELVPAPATDDRWLKMRTGTGPAGALKREIFSDKESLWGY
AASHCRLDLDRLISAGQFTWQGCHELLARHGLLLRRQHQGLAVVDAYHPRSTGVKASHVHPDLTLARAEP
HAGRFEEVAADIFERVQVTHRYDPEQAVSDRDIPGMKRDPELRRQRREARAAARQDLRARYELWRESWRK
PDLRYGERSGEIHQTCRMKKAHARVQFRDPLVRKLHYHIAEVQRMQALIALKQSVREERLTLVEAGKWYP
PTFRQWVAGEAMKGDKAAISQLRGWDYRSRRNPAASRTTDTRCVVICEPGGEPVYNHFPVSGLHATLKHN
GRVLFTKKKTGEYVCTDYGDRVVFRNHEDYETFKNVLVKTSPILFSRPEDVAMAPEGENEQFNTAFAKMV
ACRNVTTGDGLEYRVGRPELDRQRLEQEERYRDMVNPRTNYAGPVEDERPRWTPPSPR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(49-312)


  Protein structure



No available structure.




Auxiliary protein

ID   7103 GenBank   WP_217076847
Name   WP_217076847_pmk1 insolico UniProt ID   _
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12673.78 Da        Isoelectric Point: 10.7053

>WP_217076847.1 plasmid mobilization protein MobA [Klebsiella sp. PL-2018]
MSDVSKKKKSETRQKTVVRTLRLSPSEDNDIRKKAEKSGMPVSTYLRKAALEKQVNARIDDTFLMELMRL
GRMQKHLFVEGKRTGDKEYASVLVAITELTTTLRNQLMEK

  Protein domains



No domain identified.



  Protein structure



No available structure.




T4CP

ID   14744 GenBank   WP_217076841
Name   trbC_MKleb_RS28050_pmk1 insolico UniProt ID   _
Length   754 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 754 a.a.        Molecular weight: 85298.27 Da        Isoelectric Point: 6.5861

>WP_217076841.1 F-type conjugative transfer protein TrbC [Klebsiella sp. PL-2018]
MNRPAVNQALIHREAWANPALEILQSVPGHALAILLSLLAGIIWPLALPLCLLFTLVAVMLFSLRRWRCP
LRVPATLDVIDPSRDRLVRKSLLPFWPTLFQYEAVQPSRADGIFYLGYRRLADIGRELWLSMDDLTRHVM
FFASTGGGKTETIYAWTINPLCWARGYTVVDGKAQNDTTRTLWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGDKARPQSNTWNPFCHSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTTFWCTRNGKAMSL
QQLRDNMPLEKLAGLYCTALDEQWPEEALTPLRNYLQDVPGFDMALVRTPSEWTEEPRKQHAYLSGQFSE
TLSTFTETFGDIFAADAGDIDLRDSIHSDRILLVMIPAMDTSSHTTSALGRMFVTQQSMILARDLGHRLE
GTDSETLEVRKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLEFALIMMAQDQERIEEQTTANNTATLM
QNAGTKIAGRVVSDEKTAKTLKSAAGEVARAQMDNLQRQDGLLGETWTDGRQVSIRMESKIRIEELIRLQ
QGEHFTLFQGDAVPSASFYIPDEEKSTPLQPLVINRYITVDAPPLTHLARLVDRTARRRLPVPENVSAVT
GVLTEKPSRRRRKTQDIRAVIDTFQSALANRQAAWTLLARFDTSPEAREEDIWKTARQILQHTSREDRRI
RYITLNAAATNPELEVAGVKQVTLPEPKSPEVRKEKASSPDPDQCEQPVVEVRF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(438-536)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 60836..96247

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKleb_RS27845 (MKleb_5462) 56040..56984 + 945 WP_217076945 toxin co-regulated pilus biosynthesis Q family protein -
MKleb_RS27850 (MKleb_5463) 56984..57424 + 441 WP_217076946 type IV pilus biogenesis protein PilM -
MKleb_RS27855 (MKleb_5464) 57436..59112 + 1677 WP_217076947 PilN family type IVB pilus formation outer membrane protein -
MKleb_RS27860 (MKleb_5465) 59105..60361 + 1257 WP_217076948 type 4b pilus protein PilO2 -
MKleb_RS27865 (MKleb_5466) 60348..60791 + 444 WP_217076949 type IV pilus biogenesis protein PilP -
MKleb_RS27870 (MKleb_5467) 60836..62359 + 1524 WP_217076962 GspE/PulE family protein virB11
MKleb_RS27875 (MKleb_5468) 62356..63450 + 1095 WP_217076951 type II secretion system F family protein -
MKleb_RS27880 (MKleb_5469) 63496..64077 + 582 WP_217076953 type 4 pilus major pilin -
MKleb_RS27885 (MKleb_5470) 64088..64594 + 507 WP_228740818 lytic transglycosylase domain-containing protein virB1
MKleb_RS27890 (MKleb_5471) 64594..65247 + 654 WP_217076956 prepilin peptidase -
MKleb_RS27895 (MKleb_5472) 65244..66593 + 1350 WP_217076958 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MKleb_RS30245 (MKleb_5473) 66611..66832 + 222 Protein_77 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MKleb_RS27900 (MKleb_5474) 67132..68283 + 1152 WP_217076959 site-specific integrase -
MKleb_RS27905 (MKleb_5475) 68304..68951 + 648 WP_217076806 hypothetical protein -
MKleb_RS27910 (MKleb_5477) 69144..69434 + 291 WP_217076808 hypothetical protein -
MKleb_RS27915 (MKleb_5478) 69640..70071 + 432 WP_217076809 DotD/TraH family lipoprotein -
MKleb_RS27920 (MKleb_5479) 70068..70898 + 831 WP_217076810 type IV secretory system conjugative DNA transfer family protein traI
MKleb_RS27925 (MKleb_5480) 70962..72110 + 1149 WP_217076811 plasmid transfer ATPase TraJ virB11
MKleb_RS27930 (MKleb_5481) 72100..72387 + 288 WP_217076812 conjugal transfer protein traK
MKleb_RS27935 (MKleb_5482) 72398..72796 + 399 WP_217076813 phospholipase D-like domain-containing protein -
MKleb_RS27940 (MKleb_5484) 72839..74004 + 1166 WP_217076029 IS3 family transposase -
MKleb_RS27945 (MKleb_5485) 74013..74198 + 186 Protein_87 phospholipase D-like domain-containing protein -
MKleb_RS27950 (MKleb_5486) 74211..77858 + 3648 WP_217076815 LPD7 domain-containing protein -
MKleb_RS27955 (MKleb_5487) 77876..78232 + 357 WP_217076816 conjugal transfer protein traL
MKleb_RS27960 (MKleb_5488) 78343..78912 + 570 WP_217076964 DotI/IcmL family type IV secretion protein traM
MKleb_RS27965 (MKleb_5489) 78962..79945 + 984 WP_217076817 DotH/IcmK family type IV secretion protein traN
MKleb_RS27970 (MKleb_5490) 79942..81219 + 1278 WP_217076818 conjugal transfer protein TraO traO
MKleb_RS27975 (MKleb_5491) 81222..81938 + 717 WP_217076819 conjugal transfer protein TraP traP
MKleb_RS27980 (MKleb_5492) 81938..82465 + 528 WP_217076820 conjugal transfer protein TraQ traQ
MKleb_RS27985 (MKleb_5493) 82534..82938 + 405 WP_217076821 DUF6750 family protein traR
MKleb_RS27990 (MKleb_5494) 82986..83780 + 795 WP_217076822 conjugal transfer protein TraT traT
MKleb_RS27995 (MKleb_5495) 84056..87100 + 3045 WP_217076823 ATP-binding protein traU
MKleb_RS28000 (MKleb_5496) 87097..87684 + 588 WP_217076825 conjugal transfer protein TraV traV
MKleb_RS28005 (MKleb_5497) 87710..88897 + 1188 WP_228740821 conjugal transfer protein TraW traW
MKleb_RS28010 (MKleb_5498) 88894..89478 + 585 WP_217076828 conjugal transfer protein TraX -
MKleb_RS28015 (MKleb_5499) 89495..89680 - 186 WP_217076832 hypothetical protein -
MKleb_RS28020 (MKleb_5500) 89691..90605 - 915 WP_217076834 Rpn family recombination-promoting nuclease/putative transposase -
MKleb_RS28025 (MKleb_5501) 90704..92875 + 2172 WP_324110093 DotA/TraY family protein traY
MKleb_RS28030 (MKleb_5502) 92942..93571 + 630 WP_217076836 plasmid IncI1-type surface exclusion protein ExcA -
MKleb_RS28035 (MKleb_5503) 93648..93872 + 225 WP_217076838 hypothetical protein -
MKleb_RS28040 (MKleb_5504) 93947..95161 + 1215 WP_217076839 conjugal transfer protein TrbA trbA
MKleb_RS28045 (MKleb_5505) 95192..96247 + 1056 WP_217076840 DsbC family protein trbB
MKleb_RS28050 (MKleb_5506) 96228..98492 + 2265 WP_217076841 F-type conjugative transfer protein TrbC -
MKleb_RS28055 (MKleb_5507) 98591..98923 + 333 WP_217076842 hypothetical protein -
MKleb_RS28060 (MKleb_5508) 99078..99674 + 597 WP_217076843 hypothetical protein -

Host bacterium

ID   20301 GenBank   NZ_CP077674
Plasmid name   pmk1 Incompatibility group   Col440I
Plasmid size   136402 bp Coordinate of oriT [Strand]   102804..102883 [+]
Host baterium   Klebsiella sp. PL-2018

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -