Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 119859
• Name: oriT_pDY18-poxtA
• Organism: Enterococcus faecium strain DY18
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_MW207668 (5674..5711 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_pDY18-poxtA
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_LIX64_RS00110_pDY18-poxtA

• ID: 12543
• GenBank: WP_207055305
• Name: Relaxase_LIX64_RS00110_pDY18-poxtA
• UniProt ID: _
• Length: 295 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 295 a.a.
• Molecular weight: 33888.09 Da
• Isoelectric Point: 9.1275

>WP_207055305.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactobacillales]
MATVKVSTTKNGTRAMNYAEKRATVKDGLNCEINNAKNEMATVRSFYNKTDNIQAHLVIQSFSHEESQQL
GAEKINHLGIELAQKIAPNHQIAVYTHTDKEHFHNHIVMNSVSFSDGSKYHQHHFFDHVKTLNDELAKEH
GLSIVTESALERKTMAEIKLEKKGQIPWKQEIRNAVDSTMGDFSVNSYERFKACLNEKGVDVTIRGKNVT
YELLDSKNKVRGVKLGEDYQKEPILTELSRRSNLGLQQTMSAEANQMRSNELRTAANQERQYQTRKKEFQ
KEIQQERYIDRGFSL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-241)

Protein structure

No available structure.

Host bacterium

• ID: 20289
• GenBank: NZ_MW207668
• Plasmid name: pDY18-poxtA
• Incompatibility group: -
• Plasmid size: 34936 bp
• Coordinate of oriT [Strand]: 5674..5711 [-]
• Host baterium: Enterococcus faecium strain DY18

Cargo genes

• Drug resistance gene: tet(L), tet(M), poxtA, fexB
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -