Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119753
Name   oriT_p2757-138 in_silico
Organism   Klebsiella variicola strain KP2757
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP060808 (79394..79443 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_p2757-138
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12482 GenBank   WP_043907038
Name   traI_IAP99_RS26975_p2757-138 insolico UniProt ID   A0A0S1TKY9
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190458.19 Da        Isoelectric Point: 6.0263

>WP_043907038.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSLSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAPGRGNDLLISRQAWDAEKSILTRVLEGKDAVAPLMDRVPDSLMTELTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

AAA domain (AAA_30)

(969-1156)

TrwC relaxase (TrwC)

(11-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

DNA helicase TraI (TraI_C)

(1432-1554)


  Protein structure



No available structure.




T4CP

ID   14664 GenBank   WP_013214038
Name   traD_IAP99_RS26970_p2757-138 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 86010.07 Da        Isoelectric Point: 5.1787

>WP_013214038.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 78836..107449

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAP99_RS26765 (IAP99_26765) 73990..74307 + 318 WP_014343505 hypothetical protein -
IAP99_RS26770 (IAP99_26770) 75148..75504 + 357 WP_014343501 hypothetical protein -
IAP99_RS26775 (IAP99_26775) 75565..75777 + 213 WP_014343500 hypothetical protein -
IAP99_RS26780 (IAP99_26780) 75788..76012 + 225 WP_014343499 hypothetical protein -
IAP99_RS26785 (IAP99_26785) 76093..76413 + 321 WP_014343498 type II toxin-antitoxin system RelE/ParE family toxin -
IAP99_RS26790 (IAP99_26790) 76403..76681 + 279 WP_004152721 helix-turn-helix transcriptional regulator -
IAP99_RS29455 76685..76789 + 105 Protein_96 transcriptional regulator -
IAP99_RS26795 (IAP99_26795) 77620..78441 + 822 WP_004152492 DUF932 domain-containing protein -
IAP99_RS26800 (IAP99_26800) 78474..78803 + 330 WP_013214015 DUF5983 family protein -
IAP99_RS26805 (IAP99_26805) 78836..79321 - 486 WP_014343495 transglycosylase SLT domain-containing protein virB1
IAP99_RS26810 (IAP99_26810) 79712..80128 + 417 WP_014343494 conjugal transfer relaxosome DNA-binding protein TraM -
IAP99_RS26815 (IAP99_26815) 80328..81065 + 738 WP_013214018 conjugal transfer protein TrbJ -
IAP99_RS26820 (IAP99_26820) 81180..81386 + 207 WP_171773970 TraY domain-containing protein -
IAP99_RS26825 (IAP99_26825) 81448..81816 + 369 WP_004194426 type IV conjugative transfer system pilin TraA -
IAP99_RS26830 (IAP99_26830) 81830..82135 + 306 WP_004144424 type IV conjugative transfer system protein TraL traL
IAP99_RS26835 (IAP99_26835) 82155..82721 + 567 WP_004144423 type IV conjugative transfer system protein TraE traE
IAP99_RS26840 (IAP99_26840) 82708..83448 + 741 WP_013214019 type-F conjugative transfer system secretin TraK traK
IAP99_RS26845 (IAP99_26845) 83448..84872 + 1425 WP_013214020 F-type conjugal transfer pilus assembly protein TraB traB
IAP99_RS26850 (IAP99_26850) 84944..85528 + 585 WP_014343493 type IV conjugative transfer system lipoprotein TraV traV
IAP99_RS26855 (IAP99_26855) 85851..86069 + 219 WP_004195468 hypothetical protein -
IAP99_RS26860 (IAP99_26860) 86070..86381 + 312 WP_013214022 hypothetical protein -
IAP99_RS26865 (IAP99_26865) 86448..86852 + 405 WP_004197817 hypothetical protein -
IAP99_RS26870 (IAP99_26870) 87230..87628 + 399 WP_014343490 hypothetical protein -
IAP99_RS26875 (IAP99_26875) 87700..90339 + 2640 WP_013214024 type IV secretion system protein TraC virb4
IAP99_RS26880 (IAP99_26880) 90339..90728 + 390 WP_013214025 type-F conjugative transfer system protein TrbI -
IAP99_RS26885 (IAP99_26885) 90728..91363 + 636 WP_013214026 type-F conjugative transfer system protein TraW traW
IAP99_RS26890 (IAP99_26890) 91398..91799 + 402 WP_004194979 hypothetical protein -
IAP99_RS26895 (IAP99_26895) 91796..92785 + 990 WP_013214027 conjugal transfer pilus assembly protein TraU traU
IAP99_RS26900 (IAP99_26900) 92798..93436 + 639 WP_004193871 type-F conjugative transfer system pilin assembly protein TrbC trbC
IAP99_RS26905 (IAP99_26905) 93495..95450 + 1956 WP_014343489 type-F conjugative transfer system mating-pair stabilization protein TraN traN
IAP99_RS26910 (IAP99_26910) 95482..95736 + 255 WP_004152674 conjugal transfer protein TrbE -
IAP99_RS26915 (IAP99_26915) 95714..95962 + 249 WP_013214029 hypothetical protein -
IAP99_RS26920 (IAP99_26920) 95975..96301 + 327 WP_013214030 hypothetical protein -
IAP99_RS26925 (IAP99_26925) 96322..97074 + 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
IAP99_RS26930 (IAP99_26930) 97085..97324 + 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
IAP99_RS26935 (IAP99_26935) 97296..97853 + 558 WP_013214031 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
IAP99_RS26940 (IAP99_26940) 97899..98342 + 444 WP_013214032 F-type conjugal transfer protein TrbF -
IAP99_RS26945 (IAP99_26945) 98320..99699 + 1380 WP_074160414 conjugal transfer pilus assembly protein TraH traH
IAP99_RS26950 (IAP99_26950) 99699..102521 + 2823 WP_013214034 conjugal transfer mating-pair stabilization protein TraG traG
IAP99_RS26955 (IAP99_26955) 102544..103146 + 603 WP_013214035 conjugal transfer protein TraS -
IAP99_RS26960 (IAP99_26960) 103397..104128 + 732 WP_013214036 conjugal transfer complement resistance protein TraT -
IAP99_RS26965 (IAP99_26965) 104321..105010 + 690 WP_014343485 hypothetical protein -
IAP99_RS26970 (IAP99_26970) 105137..107449 + 2313 WP_013214038 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   20183 GenBank   NZ_CP060808
Plasmid name   p2757-138 Incompatibility group   IncR
Plasmid size   138721 bp Coordinate of oriT [Strand]   79394..79443 [-]
Host baterium   Klebsiella variicola strain KP2757

Cargo genes

Drug resistance gene   blaCTX-M-14, sul1, blaDHA-1, qnrB4
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9