Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119740
Name   oriT_pwzx_IMP in_silico
Organism   Klebsiella oxytoca strain wzx-2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MW590809 (42904..43004 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pwzx_IMP
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12473 GenBank   WP_228729548
Name   mobF_KUG46_RS00265_pwzx_IMP insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 99842.14 Da        Isoelectric Point: 9.8672

>WP_228729548.1 MobF family relaxase [Klebsiella oxytoca]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTRGRLRKRKIKHAKPVAAAVAA

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure



No available structure.




Auxiliary protein

ID   7083 GenBank   WP_001749975
Name   WP_001749975_pwzx_IMP insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   14654 GenBank   WP_000342688
Name   t4cp2_KUG46_RS00270_pwzx_IMP insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 13127..23452

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUG46_RS00060 8972..10405 - 1434 WP_001288432 DNA cytosine methyltransferase -
KUG46_RS00065 (pB_gene0011) 10504..10776 + 273 Protein_13 IS1 family transposase -
KUG46_RS00070 10787..10993 - 207 WP_001749967 hypothetical protein -
KUG46_RS00075 10998..11645 - 648 WP_015344958 restriction endonuclease -
KUG46_RS00080 11695..11928 - 234 WP_001191790 hypothetical protein -
KUG46_RS00085 12042..12356 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
KUG46_RS00090 12353..12697 - 345 WP_001749964 hypothetical protein -
KUG46_RS00095 12713..13084 - 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
KUG46_RS00100 (pB_gene0012) 13127..13861 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
KUG46_RS00105 13870..14151 + 282 WP_000440698 transcriptional repressor KorA -
KUG46_RS00110 (pB_gene0013) 14161..14454 + 294 WP_001749962 hypothetical protein virB2
KUG46_RS00115 (pB_gene0014) 14504..14821 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
KUG46_RS00120 (pB_gene0015) 14821..17421 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
KUG46_RS00125 (pB_gene0016) 17439..18152 + 714 WP_001749960 type IV secretion system protein virB5
KUG46_RS00130 (pB_gene0017) 18160..18387 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
KUG46_RS00135 (pB_gene0018) 18403..19443 + 1041 WP_001749958 type IV secretion system protein virB6
KUG46_RS00140 19514..19672 + 159 WP_012561180 hypothetical protein -
KUG46_RS00145 (pB_gene0019) 19662..20360 + 699 WP_000646594 virB8 family protein virB8
KUG46_RS00150 (pB_gene0020) 20371..21255 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
KUG46_RS00155 (pB_gene0021) 21255..22415 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
KUG46_RS00160 (pB_gene0022) 22457..23452 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
KUG46_RS00165 (pB_gene0023) 23452..23985 + 534 WP_000792636 phospholipase D family protein -
KUG46_RS00420 24159..24446 - 288 Protein_34 DUF6710 family protein -
KUG46_RS00180 (pB_gene0024) 24769..25473 + 705 WP_001067855 IS6-like element IS26 family transposase -
KUG46_RS00185 25527..26158 - 632 Protein_36 transposase -
KUG46_RS00190 (pB_gene0026) 26553..27209 + 657 WP_001516695 quinolone resistance pentapeptide repeat protein QnrS1 -
KUG46_RS00195 27528..28142 - 615 WP_014839983 recombinase family protein -

Host bacterium

ID   20170 GenBank   NZ_MW590809
Plasmid name   pwzx_IMP Incompatibility group   IncN
Plasmid size   62892 bp Coordinate of oriT [Strand]   42904..43004 [+]
Host baterium   Klebsiella oxytoca strain wzx-2

Cargo genes

Drug resistance gene   sul1, blaIMP-4, qnrS1, aac(6')-IIa
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -