Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119680
Name   oriT_pBjCNPSo22b in_silico
Organism   Bradyrhizobium japonicum strain CNPSo 22
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP141638 (105983..106020 [+], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      18..24, 29..35  (ACGTCGC..GCGACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT_pBjCNPSo22b
TCCAAGGGCGCAGTAATACGTCGCTGACGCGACGTCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12438 GenBank   WP_232674620
Name   traA_R3F73_RS47470_pBjCNPSo22b insolico UniProt ID   _
Length   1102 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1102 a.a.        Molecular weight: 122893.31 Da        Isoelectric Point: 10.2929

>WP_232674620.1 Ti-type conjugative transfer relaxase TraA [Bradyrhizobium japonicum]
MAITHFTPQLIGRGDGRSAVLAAAYRHCAKMEFEAEGRTVDYSNKRGMIHEEFLVPADAPAWLRTMIADR
SVAGASEAFWNKVEAFEKRADAQFAKEFIIALPVELTSEQNIALMRQFVVEQVLSRGQVADWVYHDEPGN
PHVHMMTTLRPLTEDGFGPKRVAVIGEDGQPLRMKSGKIAYRLWAGDKQEFLEQRNGWLDLQNQHLALNG
HEIRVDGRSYAERGIDVVPTTHIGVAAKAIQRRAEREGRDPNLERLRVFNENRAETLRRIQRRPEIVLDM
ISREKSVFDERDVGKLLHRYVDDAATFQHLMARVLQSPDVLQLEGEQVDFATGARAPAKYTTRELIRLEA
EMVRRAVHLSGSGAFPVKPSVLAAMSARHANLSAEQRAAIAHLASGIRIAAVVGRAGAGKTTMMRAAREV
WEAAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKSGRDGLDDRTVFVLDEAGMVASRQMALFVE
TASKAGAKLVLVGDPEQLQPIEAGAAFRAIVERIGYAELETIYRQRQQWMRDASLDLARGRTAQALSAYR
QQGRVFGSELKAQAIDNLIADWNRDYDPSKSTLILAHLRRDIRALNEMARAKLVEGGLLEQGHAFRTEDG
ERRFAPGDQIVFLKNEGSLGVKNGMIGKVVEAGTARLVAEIGEGECRCRVEVDQRFYRNIDHGYATTVHK
AQGATVDRVKVLATLSLDKHLAYVALTRHREDVALYYGRRSFQKAGGLIPILSQRRAKETTLDYERGALY
RAALRFAANRGLHVVRVARTLVNDRLRWTVRQKQRLAEVARRLRALAEKLGLVDGRKPIISTAHKEAKPM
IAGVTTFPKSLIDTVEDRILADPAIKKQWEDVSIRFRLVFADPERAFRATNFDAMLKDPAAASSTMARLA
SSPESFGALRGKVGMLAGKSDKQVRQRAEANAPALKRDIERYLRLRAEAEHKHEAEERAIRHRVAIDIPA
LSREARRVLERVRDAIDRNDLPSALEFALADRMVKAEIDGFNKAVAERFGDRTFLSNAAREPNGQPFEKA
AAGMDPARREQLKEAWPAMRAAQQLAAGERTAQALKQAEGQRLTQSQGQTLK

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(382-565)

MobA/MobL family (MobA_MobL)

(17-243)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(858-954)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(694-735)


  Protein structure



No available structure.




T4CP

ID   14606 GenBank   WP_232674619
Name   traG_R3F73_RS47455_pBjCNPSo22b insolico UniProt ID   _
Length   643 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 643 a.a.        Molecular weight: 69692.74 Da        Isoelectric Point: 9.6771

>WP_232674619.1 Ti-type conjugative transfer system protein TraG [Bradyrhizobium japonicum]
MRANKIALAIVPAVLMASLCLVLTGIEAWLSKFGTTPNARLVLGRVGIALPYVVAAAIGVVFLLAAAGAT
AIRSAGWSVVFGGLLVVAIAVSREIARPHSFANALLAGDRLLAYADPSTIIGATIAALVAVFGLRVAIKG
NAAFAAAAPRRIRGRRAIHGESDWMVMTEAARLFPETGGIVVGERYRVDRDAVTDRSFRADDAETWGAGG
KSPLLCFDASFGSSHGIVFAGSGGFKTTSVTVPTALKWGGGLVVLDPSYEVAPMVTGHRHGAGRQVFVLD
PRSSGVGFNVLDWIGQHGATKEEDIAAVASWIMTDNPRQGSARDDFFRASALQLLTALIGDVCLSGHTEK
KDQHLRQVRANLSEPEPKLRQRLQTIYDNSESGFVKENVAPFIAMTPETFSGVYANAVKETHWLSYANYA
VLVSGSSFTTAELAEGRTDVFINLDLKTLENHAGLARVVIGSLLNAIYNRNGEVTGRTLFLLDEVARLGY
LRILETARDAGRKYGITLLMLYQSIGQMREAYGGRDATSKWFESASWISFSAINDPETADYISKRCGDTT
VEVDQLSRSSQMSGSSRTRSKQLARRPLMLPHEVLRMRTDEQIIFTAGNPPLRCGRAIWFRRTDMRACVG
ENRFYRREAGQRS

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(163-635)

  Protein structure



No available structure.



ID   14607 GenBank   WP_232674731
Name   t4cp2_R3F73_RS48320_pBjCNPSo22b insolico UniProt ID   _
Length   791 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 791 a.a.        Molecular weight: 88425.42 Da        Isoelectric Point: 5.4966

>WP_232674731.1 conjugal transfer protein TrbE [Bradyrhizobium japonicum]
MVALRTFRHSGPSFADLVPYAGLVANGIVLLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINTILARLG
SGWMIQVEAVRVPTTDYPAQQDCHFPDPVTRAIDDERRGRFEQERGHFESRHAIILSWRPPERRRAGLAR
YIYSDAESRSASYADTAIESFRTSIREVEQYLASVLSIRRMETREVEERDGTRIARYDELFQFIRFCITG
ENHPIRLPDIPMYLDWLATAELQHGLSPMVENRFLGVVAIDGFPAESWPGILNSLDLMPLTYRWSSRFMF
LDDQDARQKLERTRKKWQQKVRPFIDRLFQTQSRSIDQDAAAMVAETEDAIAQASSQLVAYGYYTPVIVL
FDESSARLQEKAEAVRRLIQAEGFGARIETLNATEAFLGSLPGNWYANIREPLINTRNLADLIPLNSVWS
GNPTAPCPFYPPSSPPLMQVASGSTPFRLNLHVDDVGHTLVFGPTGSGKSTLPLTLAAGGDHYEIGGDDA
DGAAALAFCPLSDLSTDNDRAWAAEWVETLVAVQGVLITPDHRNAISRQIGLMAEARGRSLSDFVSGVQM
REIKDALHHYTVDGPMGQLLDAERDGLQLGSFQTFEIEQLMNMGERNLVPVLTYLFRRIEKRLTGSPSLI
ILDEAWLMLGHPVFRDKIREWLKVLRKANCAVLLATQSISDAERSGIIDVLKESCPTKICLPNGAAREPG
TREFYERIGFNERQIEIVAAASPKREYYVASPEGRRLFNMALGPVALSFVGASGKEDLKRILALKAAEAN
WPVHWLKERGIGHAETLLADL

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(191-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 279720..289198

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R3F73_RS48270 (R3F73_48270) 274850..276193 + 1344 WP_253575149 IS1380-like element ISBdi2 family transposase -
R3F73_RS48275 (R3F73_48275) 276451..277053 + 603 WP_028152988 hypothetical protein -
R3F73_RS48280 (R3F73_48280) 277179..277463 + 285 WP_155258061 hypothetical protein -
R3F73_RS48285 (R3F73_48285) 278270..279718 + 1449 WP_232674895 IS4 family transposase -
R3F73_RS48290 (R3F73_48290) 279720..281027 - 1308 WP_232674733 IncP-type conjugal transfer protein TrbI virB10
R3F73_RS48295 (R3F73_48295) 281039..281491 - 453 WP_028152991 conjugal transfer protein TrbH -
R3F73_RS48300 (R3F73_48300) 281494..282333 - 840 WP_038944961 P-type conjugative transfer protein TrbG virB9
R3F73_RS48305 (R3F73_48305) 282351..283013 - 663 WP_028152993 conjugal transfer protein TrbF virB8
R3F73_RS48310 (R3F73_48310) 283057..284214 - 1158 WP_028152994 P-type conjugative transfer protein TrbL virB6
R3F73_RS48315 (R3F73_48315) 284414..285211 - 798 WP_232674732 P-type conjugative transfer protein TrbJ virB5
R3F73_RS48320 (R3F73_48320) 285183..287558 - 2376 WP_232674731 conjugal transfer protein TrbE virb4
R3F73_RS48325 (R3F73_48325) 287568..287867 - 300 WP_232674730 conjugal transfer protein TrbD virB3
R3F73_RS48330 (R3F73_48330) 287860..288240 - 381 WP_028152998 TrbC/VirB2 family protein virB2
R3F73_RS48335 (R3F73_48335) 288230..289198 - 969 WP_232674729 P-type conjugative transfer ATPase TrbB virB11
R3F73_RS48340 (R3F73_48340) 289204..289830 - 627 WP_232674728 acyl-homoserine-lactone synthase -

Host bacterium

ID   20110 GenBank   NZ_CP141638
Plasmid name   pBjCNPSo22b Incompatibility group   -
Plasmid size   290242 bp Coordinate of oriT [Strand]   105983..106020 [+]
Host baterium   Bradyrhizobium japonicum strain CNPSo 22

Cargo genes

Drug resistance gene   -
Virulence gene   wzt
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrID1